Organic Aerosol Component (OACOMP) Value-Added Product Report

Significantly improved returns in their aerosol chemistry data can be achieved via the development of a value-added product (VAP) of deriving OA components, called Organic Aerosol Components (OACOMP). OACOMP is primarily based on multivariate analysis of the measured organic mass spectral matrix. The key outputs of OACOMP are the concentration time series and the mass spectra of OA factors that are associated with distinct sources, formation and evolution processes, and physicochemical properties

Coherent diffractive imaging of microtubules using an X-ray laser

X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature

Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser

A major goal for X-ray free-electron laser (XFEL) based science is to elucidate structures of biological molecules without the need for crystals. Filament systems may provide some of the first single macromolecular structures elucidated by XFEL radiation, since they contain one-dimensional translational symmetry and thereby occupy the diffraction intensity region between the extremes of crystals and single molecules. Here, we demonstrate flow alignment of as few as 100 filaments (Escherichia coli pili, F-actin, and amyloid fibrils), which when intersected by femtosecond X-ray pulses result in diffraction patterns similar to those obtained from classical fiber diffraction studies. We also determine that F-actin can be flow-aligned to a disorientation of approximately 5 degrees. Using this XFEL-based technique, we determine that gelsolin amyloids are comprised of stacked β-strands running perpendicular to the filament axis, and that a range of order from fibrillar to crystalline is discernable for individual α-synuclein amyloids

Offensive zones in beach volleyball: differences by gender

Gender differences in anthropometric and athletic properties (e.g. strength) as well as the different net heights (2.24 vs. 2.43 m) on the same field size (8x16 m) would be reflected in the game. The literature about differences between male and female playing characteristics in beach volleyball is scarce. Therefore, the aim of this study was to analyse the differences by gender in the use of offensive zones. Study participants were 20 players (10 female and 10 male) who took part in the European Beach Volleyball Championship 2005 and 2006. Video recordings were made of the 659 points in eight matches played. The beach volleyball court was divided into six zones (z1 to z6). The results showed that men and women use different offensive zones and also were different in the percentages of ball out (15.53 and 27.38% respectively). Concretely, men players used more 1, 2, 4 and 5 zones and women 1 and 5 zones. A few differences were observed in the percentage the ball to the net (7.73 and 5.35% respectively). An understanding of the use of offensive zones is relevant to establish specific tactical training patterns for beach volleyball

Organic Aerosol Component (OACOMP) Value-Added Product Report

Significantly improved returns in their aerosol chemistry data can be achieved via the development of a value-added product (VAP) of deriving OA components, called Organic Aerosol Components (OACOMP). OACOMP is primarily based on multivariate analysis of the measured organic mass spectral matrix. The key outputs of OACOMP are the concentration time series and the mass spectra of OA factors that are associated with distinct sources, formation and evolution processes, and physicochemical properties

Human fascicle strain behavior during twitch using ultrafast ultrasound

There is recent experimental evidence that sarcomere strain behavior is highly heterogeneous across and between muscle structures. Hence, considering the hierarchical architecture of muscles, also the behavior of all acting muscle substructures is effected. However, typical investigations are limited to ex-vivo experiments or pose serious limitations for in-vivo studies. In this work, we investigate in-vivo length changes in human muscle fascicles by means of non-invasive ultrafast ultrasound. To this end, we employ a research ultrasound system and a linear array transducer to image, in plane-wave mode, medial gastrocnemius muscles during electrically-stimulated contractions. The ultrasound-based approach presented in this study allows to measure strain distributions within muscle fascicles during contractions, where the sub-fascicle structures exhibit heterogeneous behaviors