Estudio de las rizobacterias del suelo de la taiga de Chernevaya en Siberia occidental y su posible efecto sobre el crecimiento de las plantas

El aislamiento de bacterias del suelo de varios ambientes con tasas de fertilidad excepcionalmente altas representa una oportunidad para identificar agentes prometedores para promover la producción agrícola. El objetivo del artículo es estudiar las densidades de bacterias cultivables y aislar bacterias de la rizosfera del rábano y el trigo de primavera. Las plantas se cultivaron en el experimento en maceta con suelo Chernevaya virgen, que poseía una productividad extraordinaria, y suelo forestal zonal que no demostró características similares. Se purificaron cincuenta y nueve aislados bacterianos y se evaluaron sus efectos beneficiosos sobre el crecimiento temprano del trigo. Los aislados pertenecían a los filos Proteobacteria, Actinobacteria y Firmicutes, y los géneros más representados fueron Pseudomonas , Streptomyces , Paenibacillus y Methylobacterium . Estas bacterias dominantes se utilizaron en estudios de inoculación de plantas. Las cepas promovieron un aumento significativo en la longitud y biomasa de los brotes y las raíces, por lo que pueden considerarse rizobacterias promotoras del crecimiento de las plantas [PGPR]. En cuanto a los ensayos de biotest, se seleccionaron cepas que mostraron altas capacidades de promoción del crecimiento de las plantas [PGP] para una mayor investigación. Este estudio contribuyó a aislar bacterias de un entorno natural único con potencial biotecnológico para mejorar el crecimiento de las plantas y mostró potencia para ser explotadas como bioinoculantes

Three classes of ligands each bind to distinct sites on the orphan G protein-coupled receptor GPR84

Medium chain fatty acids can activate the pro-inflammatory receptor GPR84 but so also can molecules related to 3,3′-diindolylmethane. 3,3′-Diindolylmethane and decanoic acid acted as strong positive allosteric modulators of the function of each other and analysis showed the affinity of 3,3′-diindolylmethane to be at least 100 fold higher. Methyl decanoate was not an agonist at GPR84. This implies a key role in binding for the carboxylic acid of the fatty acid. Via homology modelling we predicted and confirmed an integral role of arginine172, located in the 2nd extracellular loop, in the action of decanoic acid but not of 3,3′-diindolylmethane. Exemplars from a patented series of GPR84 antagonists were able to block agonist actions of both decanoic acid and 3,3′-diindolylmethane at GPR84. However, although a radiolabelled form of a related antagonist, [3H]G9543, was able to bind with high affinity to GPR84, this was not competed for by increasing concentrations of either decanoic acid or 3,3′-diindolylmethane and was not affected adversely by mutation of arginine172. These studies identify three separable ligand binding sites within GPR84 and suggest that if medium chain fatty acids are true endogenous regulators then co-binding with a positive allosteric modulator would greatly enhance their function in physiological settings

Artificial Intelligence

The article forms a matrix of the main propositions and markers of artificial intelligence in non-professional (philistine) discourses. The study is implemented on the Internet using special tools. The subject of the analysis is the search queries in the main ‘Google’ and ‘Yandex’ services, thematic communities, social networks and users’ comments. The definition of the matrix of non-professional stereotypical labeling of artificial intelligence as an actual developing technology allows us to see a picture of a new metaphysics. “Technological” metaphysics is inextricably linked with mythological thinking and significantly affects the absorption of scientific and technological developments. It also influences the constructive critical attitude towards the physical condition. The article substantiates that this modern technical mythology, which includes many speculative assumptions, has a double meaning: on the one hand, it “domesticates” technology, and on the other hand, it creates an insurmountable barrier for the convergence of spiritual and religious scope and the scientific worldview. The definition and subsequent refinement of the mythological matrix is necessary for the effective implementation of innovative programs, adjustment of those to the education system, constructive dialogue between the state, scientists, and users

How Morphology of the Human Pluripotent Stem Cells Determines the Selection of the Best Clone

The application of patient-specific human induced pluripotent stem cells (hiPSCs) has a great perspective for the development of personalized medicine. More than 10 hiPSCs clones can be obtained from one patient but not all of them are able to undergo directed differentiation with the same efficiency. Beside, some clones are even refractory to certain directions of differentiation. Therefore, the selection of the “best” or “true” hiPSC clone is very important, but this remains a challenge. Currently, this selection is based mostly on the clone’s morphological characteristics. Earlier, using methods of mathematical analysis and deep machine learning, we showed the fundamental possibility for selecting the best clone with about 89% accuracy based on only two to three morphological features. In this chapter, we will expand on how the morphological characteristics of various hiPSCs clones, the so-called “morphological portrait,” are reflected by their proteome. By reviewing previously published data and providing the new results, we will highlight which cytoskeletal proteins are responsible for the establishment of the “good” morphological phenotype. Finally, we will suggest further directions in this research area

Low-dimensional pseudoboehmite structures for microorganism adsorption

Low-dimensional structures consisting of pseudoboehmite nanopetals are synthesized by hydrolysis of electroexplosive aluminum nanopowder. The physical and chemical properties of the synthesized nanostructures are studied, such as morphology, particle size, specific surface area, phase composition, and zeta-potential. The production of a hybrid material on the basis of cellulose acetate microfibers and low-dimensional pseudoboehmite structures is described. Using the example of gram-negative and gram-positive bacteria, we show the microorganism adsorption capacity of pseudoboehmite nanopetals and the hybrid material