The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers

Background/Aims: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease that significantly increases the risk of fetal complications. Here, we measured serum miRNA levels in ICP patients to identify candidate biomarkers for ICP. Methods: We used the Agilent miRNA array followed by reverse transcription-polymerase chain reaction assays to identify and validate the serum miRNA profiles of 40 pregnant women with ICP and 40 healthy pregnant controls. We used bioinformatics to identify metabolic processes related to differentially expressed miRNAs. Results: The expression levels of three miRNAs (miR-371a- 5p, miR-6865-5p, and miR-1182) were significantly increased in ICP patients compared to controls; the areas under the receiver operating characteristic (ROC) curves (AUCs) were 0.771, 0.811, and 0.798, respectively. Multiple logistic regression analysis showed that a combination of the levels of the three miRNAs afforded a greater AUC (0.845), thus more reliably diagnosing ICP. The levels of all three miRNAs were positively associated with that of total bile acids. Furthermore, bioinformatics analysis indicated that the three miRNAs principally affected lipid phosphorylation, apoptosis, and the MAPK signaling pathway. Conclusion: This preliminary work improves our understanding of serum miRNA changes in pregnant women with ICP. The three miRNAs may serve as novel noninvasive biomarkers of ICP

Preparation of Metallic Iron Powder from Pyrite Cinder by Carbothermic Reduction and Magnetic Separation

The reduction and magnetic separation procedure of pyrite cinder in the presence of a borax additive was performed for the preparation of reduced powder. The effects of borax dosage, reduction temperature, reduction time and grinding fineness were investigated. The results show that when pyrite cinder briquettes with 5% borax were pre-oxidized at 1050 °C for 10 min, and reduced at 1050 °C for 80 min, with the grinding fineness (<0.44 mm) passing 81%, the iron recovery was 91.71% and the iron grade of the magnetic concentrate was 92.98%. In addition, the microstructures of the products were analyzed by optical microscope, scanning electron microscope (SEM), and mineralography, and the products were also studied by the X-ray powder diffraction technique (XRD) to investigate the mechanism; the results show that the borax additive was approved as a good additive to improve the separation of iron and gangue

Experiments and numerical simulations on dynamic crack behavior at the interface of layered brittle material

Crack behavior at the interface between two materials is the core problem of layered material fracturing. In this paper, first, experimental tests were conducted on layered material using a drop weight test system following the caustics method. The layered material was made of poly (methyl methacrylate) (PMMA) and epoxy resin bonded with Loctite-330 at two inclination angles (30 degrees and 60 degrees). A corresponding numerical simulation was carried out using continuum-discontinuum element methods. Crack propagation is found to mainly occur in mode I in the PMMA and epoxy resin but follows a mixed cracking mode at the interface. The fracture parameters of the crack tip in the layered materials changed substantially owing to the existence and change of the interface structure. After the crack enters the interface, the crack propagation speed increases significantly. Higher crack dip angles are associated with greater crack propagation speed increases after entering the interface. The simulation results indicate that the interface strength properties affect crack behavior at the interface. This effect also varies as a function of interface inclination and impact velocity

Quality evaluation methods of chinese medicine based on scientific supervision: recent research progress and prospects

Abstract Traditional Chinese medicine (TCM) is increasingly getting attention worldwide, as it has played a very satisfactory role in treating COVID-19 during these past 3 years, and the Chinese government highly supports the development of TCM. The therapeutical theory and efficacies of Chinese medicine (CM) involve the safety, effectiveness and quality evaluation of CM, which requires a standard sound system. Constructing a scientific and reasonable CM quality and safety evaluation system, and establishing high-quality standards are the key cores to promote the high-quality development of CM. Through the traditional quality control methods of CM, the progress of the Q-marker research and development system proposed in recent years, this paper integrated the research ideas and methods of CM quality control and identified effective quality parameters. In addition, we also applied these effective quality parameters to create a new and supervision model for the quality control of CM. In conclusion, this review summarizes the methods and standards of quality control research used in recent years, and provides references to the quality control of CM and how researchers conduct quality control experiments

Bissubvilides A and B, Cembrane-Capnosane Heterodimers from the Soft Coral Sarcophyton subviride

K

Hybrid Organic-Inorganic Thermoelectric Materials and Devices

Hybrid Organic-Inorganic Thermoelectric Materials and Device