Application of Grouting Water Plugging Technology in Enriched Water Mountain Tunnel

In the expressway built in Chongqing northeast region, the tunnel occupies a large proportion in the total mileage. Long mountain tunnels with large buried depth and complex geological conditions are common in this area, which are prone to water gusher disasters during construction, which seriously threaten the construction quality and affect the operation of the expressway after it is completed and opened to traffic. In this paper, Jixinling Tunnel under construction in Wuzhen Expressway is taken as an example. Combined with advanced geological forecast data and site conditions, appropriate grouting materials and reasonable and feasible construction technology are selected for the surrounding advanced curtain grouting reinforcement and water plugging treatment, which effectively controls the water gushing disaster of the tunnel and ensures the safety of the construction process. It can provide some reference for the treatment of water gushing disease in mountain tunnel under similar geological conditions

Reflections on the Information Management System for Highway Tunnel Construction

In the process of tunnel construction in China, various accidental and unforeseen factors arise, making it a complex and intersecting endeavor involving multiple stakeholders. Safety risks are particularly prominent in tunnel construction due to its inherent nature. With the advancement of information technology, intelligent systems, and the Internet, leveraging advanced internet technologies becomes feasible for comprehensive monitoring of the entire tunnel construction process and dynamic management of all relevant information. This facilitates the intelligent and information of tunnel construction, reduces quality and safety risks, and elevates the level of tunnel construction management. This paper provides a thoughtful design for the information system of tunnel construction, aiming to serve as a reference for the development of information in tunnel engineering

Application Analysis of Ecological Slope Protection Technology for Mountain Highway Slope

With the standard of living in China is improved constantly, the construction of expressway in northeast Chongqing has also witnessed a rapid growth. The process of the construction of mountain road, the cuttings and embarkment of the road would definitely cause the distortion and movement of the high slope and create a large amount of lose sand, which seriously threat the safety of high slopes. Meanwhile, the engineering also causes destruction the original vegetation, which is hard to restore. Therefore, in the construction of expressway in mountainous areas, we should hold on to the idea of green development and adopt the combination of engineering protection and vegetational protection. The project should be revised constantly according to the situation and reduce its negative affect on ecological environment as much as possible

Genome-wide characterization of GRAS family genes in Medicago truncatula reveals their evolutionary dynamics and functional diversification.

The GRAS gene family is a large plant-specific family of transcription factors that are involved in diverse processes during plant development. Medicago truncatula is an ideal model plant for genetic research in legumes, and specifically for studying nodulation, which is crucial for nitrogen fixation. In this study, 59 MtGRAS genes were identified and classified into eight distinct subgroups based on phylogenetic relationships. Motifs located in the C-termini were conserved across the subgroups, while motifs in the N-termini were subfamily specific. Gene duplication was the main evolutionary force for MtGRAS expansion, especially proliferation of the LISCL subgroup. Seventeen duplicated genes showed strong effects of purifying selection and diverse expression patterns, highlighting their functional importance and diversification after duplication. Thirty MtGRAS genes, including NSP1 and NSP2, were preferentially expressed in nodules, indicating possible roles in the process of nodulation. A transcriptome study, combined with gene expression analysis under different stress conditions, suggested potential functions of MtGRAS genes in various biological pathways and stress responses. Taken together, these comprehensive analyses provide basic information for understanding the potential functions of GRAS genes, and will facilitate further discovery of MtGRAS gene functions

Application of "Problem Solving" in Religious Educatiion

Anglická anotace (English annotation) Application of the "Problem solving" to the Religious education This dissertation work deals with the practical application of teaching methods "problem solving" in the lessons of religion with all its pros and cons. It deals with issues in the teaching of religious education 8th classes of elementary schools, using only the Czech language and suggested topics, which are set out in the curriculum of religious education Roman Catholic Church in 2004, for use in the diocese of Hradec Králové. The first part is devoted to learning theory, regulations, curriculum and teaching plans of the relevant legislation with regard to the specifics and the territorial limits. The following section presents the individual applicable methods in the "problem solving" with respect to the development of key competencies of students. And in the last section are selected appropriate methods discussed in details and presented on topics discussed in the teaching of religious education lessons. The course of instruction and the subsequent reactions of students and teachers alike are drawn appropriate recommendations to the methods already fully applicable. In conclusion, the proper methods for teaching religious education in the 8th elementary school classroom are described in detail for any..

MOESM5 of Switchgrass SBP-box transcription factors PvSPL1 and 2 function redundantly to initiate side tillers and affect biomass yield of energy crop

Additional file 5: Figure S3.  Expression levels of PvSPL1 in PvSPL1SRDX and rPvSPL1 overexpressing transgenic switchgrass plants were revealed by qRT-PCR. Switchgrass Ubq2 was used as the reference for normalization. PvSPL1: sum of exo- and endo-PvSPL1 transcript versions

Data_Sheet_1_Association between microbiological risk factors and neurodegenerative disorders: An umbrella review of systematic reviews and meta-analyses.PDF

The role of microbiological factors in the development of neurodegenerative diseases is attracting increasing attention, while the relationship remains debated. This study aimed to comprehensively summarize and evaluate the associations between microbiological factors and the risk of neurodegenerative disorders with an umbrella review. PubMed, Embase, and the Cochrane library were used to search for papers from the earliest to March 2021 for identifying meta-analyses and systematic reviews that examined associations between microbiological factors and neurodegenerative diseases. AMSTAR2 tool was employed to evaluate the methodical quality of systematic reviews and meta-analyses. The effect size and 95% confidence interval (95% CI) were recalculated with a random effect model after the overlap was recognized by the corrected covered area (CCA) method. The heterogeneity of each meta-analysis was measured by the I2 statistic and 95% prediction interval (95% PI). Additionally, publication bias and the quality of evidence were evaluated for all 37 unique associations. Only 4 associations had above the medium level of evidence, and the rest associations presented a low level of evidence. Among them, helicobacter pylori (HP), infection, and bacteria are associated with Parkinson's disease (PD), and the other one verifies that periodontal disease is a risk factor for all types of dementia. Following the evidence of our study, eradication of HP and aggressive treatment of periodontitis are beneficial for the prevention of PD and dementia, respectively. This umbrella review provides comprehensive quality-grade evidence on the relationship between microbial factors and neurodegenerative disease. Regardless of much evidence linking microbial factors to neurodegenerative diseases, these associations are not necessarily causal, and the evidence level is generally low. Thus, more effective studies are required.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/#searchadvanced, PROSPERO, identifier: CRD42021239512.</p

MOESM4 of Switchgrass SBP-box transcription factors PvSPL1 and 2 function redundantly to initiate side tillers and affect biomass yield of energy crop

Additional file 4: Figure S2. Relationships between expression levels of the miR156-targeted PvSPL genes and tiller numbers. Twelve independent positive miR156 overexpressing transgenic switchgrass lines were generated by Agrobacterium-mediated transformation. Expression levels of PvSPL1/19, 2/18, 3/5, and 6/7 in the transgenic switchgrass plants were detected by qRT-PCR. Switchgrass Ubq2 was used as the reference for normalization

MOESM7 of Switchgrass SBP-box transcription factors PvSPL1 and 2 function redundantly to initiate side tillers and affect biomass yield of energy crop

Additional file 7: Table S4. Primer sequences used for gene expression analysis by qRT-PCR and plasmid construction