Neural oscillations during acupuncture imagery partially parallel that of real needling

IntroductionTasks involving mental practice, relying on the cognitive rehearsal of physical motors or other activities, have been reported to have similar patterns of brain activity to overt execution. In this study, we introduced a novel imagination task called, acupuncture imagery and aimed to investigate the neural oscillations during acupuncture imagery.MethodsHealthy volunteers were guided to watch a video of real needling in the left and right KI3 (Taixi point). The subjects were then asked to perform tasks to keep their thoughts in three 1-min states alternately: resting state, needling imagery left KI3, and needling imagery right KI3. Another group experienced real needling in the right KI3. A 31-channel-electroencephalography was synchronously recorded for each subject. Microstate analyses were performed to depict the brain dynamics during these tasks.ResultsCompared to the resting state, both acupuncture needling imagination and real needling in KI3 could introduce significant changes in neural dynamic oscillations. Moreover, the parameters involving microstate A of needling imagery in the right KI3 showed similar changes as real needling in the right KI3.DiscussionThese results confirm that needling imagination and real needling have similar brain activation patterns. Needling imagery may change brain network activity and play a role in neural regulation. Further studies are needed to explore the effects of acupuncture imagery and the potential application of acupuncture imagery in disease recovery

Adaptability analysis and model development of various LS-factor formulas in RUSLE model: A case study of Fengyu River Watershed, China

The slope length and slope steepness factor (LS-factor) formula in the Revised Universal Soil Loss Equation (RUSLE) has a considerable level of uncertainty due to the existence of multiple methods. In this study, four commonly used formulas for the slope length factor and two formulas for the slope gradient factor were chosen and combined based on their applicability to the specific research context. Based on the ModelBuilder in ArcGIS Pro 3.0, a RUSLE calculation model (RUSLE-Cal model) was constructed in the study, which can automatically calculate the soil erosion modulus using four commonly used RUSLE formulas and one combination formula. Taking the Fengyu River watershed in China as a case study, this research analyzes the uncertainty of different LS-factor formulas and validates the accuracy of RUSLE simulation results using measured sediment data. The optimal combination of LS-factor formulas is selected, and an in-depth analysis is conducted on the origins and suitability of each formula. The accuracy validation results indicate that, for the Fengyu River watershed, the optimal combination of L-factor and S-factor formulas were determined based on the slope gradient. Specifically, L1 formula was used when slope ≤ 10°, and L3 formula was used when slope greater than 10°. Similarly, S1 formula was used when slope ≤ 18°, and S2 formula was used when slope greater than 18°. The RUSLE model achieved the best simulation results with a relative error of 5.55%. The results of the uncertainty analysis indicate that the four formulas have a significant impact on the simulated soil erosion, with a RE ranging from −99.18% to 31.49%. Therefore, based on literature review and formula analysis, a suitability selection table for L-factor and S-factor formulas is provided, which can provide formula basis for the improvement of soil erosion in watershed models

Data_Sheet_1_Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain.docx

Visualizing the relationships and interactions among different biological components in the whole brain is crucial to our understanding of brain structures and functions. However, an automatic multicolor whole-brain imaging technique is still lacking. Here, we developed a multicolor wide-field large-volume tomography (multicolor WVT) to simultaneously acquire fluorescent signals in blue, green, and red channels in the whole brain. To facilitate the segmentation of brain regions and anatomical annotation, we used 4′, 6-diamidino-2-phenylindole (DAPI) to provide cytoarchitecture through real-time counterstaining. We optimized the imaging planes and modes of three channels to overcome the axial chromatic aberration of the illumination path and avoid the crosstalk from DAPI to the green channel without the modification of system configuration. We also developed an automatic contour recognition algorithm based on DAPI-staining cytoarchitecture to shorten data acquisition time and reduce data redundancy. To demonstrate the potential of our system in deciphering the relationship of the multiple components of neural circuits, we acquired and quantified the brain-wide distributions of cholinergic neurons and input of ventral Caudoputamen (CP) with the anatomical annotation in the same brain. We further identified the cholinergic type of upstream neurons projecting to CP through the triple-color collocated analysis and quantified its proportions in the two brain-wide distributions. Both accounted for 0.22%, implying CP might be modulated by non-cholinergic neurons. Our method provides a new research tool for studying the different biological components in the same organ and potentially facilitates the understanding of the processing mechanism of neural circuits and other biological activities.</p

DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells

Abstract Background DNA methylation is a heritable epigenetic mark, enabling stable but reversible gene repression. In mammalian cells, DNA methyltransferases (DNMTs) are responsible for modifying cytosine to 5-methylcytosine (5mC), which can be further oxidized by the TET dioxygenases to ultimately cause DNA demethylation. However, the genome-wide cooperation and functions of these two families of proteins, especially at large under-methylated regions, called canyons, remain largely unknown. Results Here we demonstrate that DNMT3A and TET1 function in a complementary and competitive manner in mouse embryonic stem cells to mediate proper epigenetic landscapes and gene expression. The longer isoform of DNMT3A, DNMT3A1, exhibits significant enrichment at distal promoters and canyon edges, but is excluded from proximal promoters and canyons where TET1 shows prominent binding. Deletion of Tet1 increases DNMT3A1 binding capacity at and around genes with wild-type TET1 binding. However, deletion of Dnmt3a has a minor effect on TET1 binding on chromatin, indicating that TET1 may limit DNA methylation partially by protecting its targets from DNMT3A and establishing boundaries for DNA methylation. Local CpG density may determine their complementary binding patterns and therefore that the methylation landscape is encoded in the DNA sequence. Furthermore, DNMT3A and TET1 impact histone modifications which in turn regulate gene expression. In particular, they regulate Polycomb Repressive Complex 2 (PRC2)-mediated H3K27me3 enrichment to constrain gene expression from bivalent promoters. Conclusions We conclude that DNMT3A and TET1 regulate the epigenome and gene expression at specific targets via their functional interplay

Additional file 2: of DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells

Table S1. List of oligonucleotides: guide sgRNAs and primers. (PDF 52 Kb

Additional file 1: of DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells

Figure S1–S11. Figures and legends for Supplementary Figures S1–S11. (PDF 15.6 Mb

Nuclear mass table in deformed relativistic Hartree–Bogoliubov theory in continuum, I: Even–even nuclei

© 2022 Elsevier Inc.Ground-state properties of even–even nuclei with 8≤Z≤120 from the proton drip line to the neutron drip line have been investigated using the deformed relativistic Hartree–Bogoliubov theory in continuum (DRHBc) with the density functional PC-PK1. With the effects of deformation and continuum included simultaneously, 2583 even–even nuclei are predicted to be bound. The calculated binding energies, two-nucleon separation energies, root-mean-square (rms) radii of neutron, proton, matter, and charge distributions, quadrupole deformations, and neutron and proton Fermi surfaces are tabulated and compared with available experimental data. The rms deviation from the 637 mass data is 1.518 MeV, providing one of the best microscopic descriptions for nuclear masses. The drip lines obtained from DRHBc calculations are compared with other calculations, including the spherical relativistic continuum Hartree–Bogoliubov (RCHB) and triaxial relativistic Hartree–Bogoliubov (TRHB) calculations with PC-PK1. The deformation and continuum effects on the limits of the nuclear landscape are discussed. Possible peninsulas consisting of bound nuclei beyond the two-neutron drip line are predicted. The systematics of the two-nucleon separation energies, two-nucleon gaps, rms radii, quadrupole deformations, potential energy curves, neutron densities, neutron mean-field potentials, and pairing energies in the DRHBc calculations are also discussed. In addition, the α decay energies extracted are in good agreement with available data.11Nsciescopu