1965 Volume 14 No. 2

https://engagedscholarship.csuohio.edu/lawpublications_gavel1960s/1032/thumbnail.jp

The paleoclimatic footprint in the soil carbon stock of the Tibetan permafrost region

Data and code availability The authors declare that the majority of the data supporting the findings of this study are available through the links given in the paper. The unpublished data are available from the corresponding author upon request. The new estimate of Tibetan soil carbon stock and R code are available in a persistent repository (https://figshare.com/s/4374f28d880f366eff6d). Acknowledgements This study was supported by the Strategic Priority Research Program (A) of the Chinese Academy of Sciences (XDA20050101), the National Natural Science Foundation of China (41871104), Key Research and Development Programs for Global Change and Adaptation (2017YFA0603604), International Partnership Program of the Chinese Academy of Sciences (131C11KYSB20160061) and the Thousand Youth Talents Plan project in China. Jinzhi Ding acknowledges the General (2017M620922) and the Special Grade (2018T110144) of the Financial Grant from the China Postdoctoral Science Foundation.Peer reviewedPublisher PD

Decadal soil carbon accumulation across Tibetan permafrost regions

Acknowledgements We thank the members of Peking University Sampling Teams (2001–2004) and IBCAS Sampling Teams (2013–2014) for assistance in field data collection. We also thank the Forestry Bureau of Qinghai Province and the Forestry Bureau of Tibet Autonomous Region for their permission and assistance during the sampling process. This study was financially supported by the National Natural Science Foundation of China (31670482 and 31322011), National Basic Research Program of China on Global Change (2014CB954001 and 2015CB954201), Chinese Academy of Sciences-Peking University Pioneer Cooperation Team, and the Thousand Young Talents Program.Peer reviewedPostprintPostprin

LncRNA ADAMTS9-AS1 knockdown restricts cell proliferation and EMT in non-small cell lung cancer

A recent bioinformatics analysis identified long non‐coding RNA antisense 1 ADAMTS9-AS1 as an independent prognostic marker in several tumors, including prostate cancer and bladder cancer. Nevertheless, the prognostic value and functional role of ADAMTS9-AS1 in non-small cell lung cancer (NSCLC) remain elusive. Here, we first found that the expression of ADAMTS9-AS1 was significantly upregulated in NSCLC tissues compared with adjacent normal tissues using quantitative real time PCR analysis. Clinically, we observed that ADAMTS9-AS1 expression was associated with TNM stage, lymph node metastasis and poor prognosis in NSCLC patients. By performing lossof-function assay in A549 and 95D cells, our in vitro experiments further showed that knockdown of ADAMTS9-AS1 remarkedly suppressed cell proliferation, caused cell cycle G0/G1 arrest and apoptosis, and inhibited cell migration and invasion in NSCLC cells using CCK-8, colony formation, flow cytometry and transwell assays. Moreover, we found that ADAMTS9-AS1 knockdown downregulated the expression of CDK4, N-cadherin, Vimentin, but upregulated the expression of Bad and E-cadherin. In summary, our results revealed that ADAMTS9-AS1 may serve as a potential therapeutic target for the treatment of patients with NSCL

Effect of Composite Impregnation on Properties of Recycled Coarse Aggregate and Recycled Aggregate Concrete

To improve the properties of recycled aggregate concrete, single and composite impregnation treatments were carried out on recycled coarse aggregates with sodium silicate solution, silane slurry, and polyvinyl alcohol solution. The effects of the three chemical modifiers and different impregnation methods on the apparent density, water absorption, and crushing index of recycled coarse aggregates, as well as the basic properties of recycled aggregate concrete, were investigated. Additionally, the microstructure of the surface of recycled coarse aggregate and the interior of recycled aggregate concrete were analyzed by scanning electron microscopy. The experimental results show that the water absorption of recycled coarse aggregate soaked in polyvinyl alcohol solution decreases most significantly, reaching 64.56%. Only the combination of sodium silicate and silane impregnation produces a positive compounding effect, with a significant increase in the apparent density and a significant reduction in the crushing index of the recycled coarse aggregate. Compared with untreated concrete, the slump, compressive strength, splitting tensile strength, and flexural strength of recycled aggregate concrete prepared by sodium silicate and silane composite impregnation are increased by 9.8%, 26.53%, 21.70%, and 14.72%, respectively. The microstructure analysis shows that the composite impregnation treatment of sodium silicate and silane is most conducive to filling the cracks and holes on the surface of recycled coarse aggregate, which makes the interfacial transition zone of recycled aggregate concrete more compact and the structure more stable

Effect of Nano-TiO2 on Capillary Water Absorption of Recycled Aggregate Concrete

To improve the durability performance of recycled aggregate concrete in actual use, this paper uses nano-TiO2-modified recycled coarse aggregate to study, through experiments, the effects of nano-TiO2 on the pore distribution of recycled coarse aggregate concrete after freeze–thaw. The capillary-water-absorption law was used as the evaluation index. The recycled coarse aggregate concrete was prepared with different contents of nano-TiO2, and changes in the 24 h capillary water absorption and porosity of the recycled aggregate concrete after freeze–thaw cycles were analysed. With the help of high-resolution image recognition and binary-image-processing technology, the pore distribution of the recycled aggregate concrete before and after freeze–thaw cycles was obtained. Through the analysis of the water-absorption data at different times, the initial capillary-water-absorption rate, S1, is obtained. The capillary water absorption of recycled aggregate concrete is reacted with S1, and the initial capillary-water-absorption prediction model of nano-TiO2 recycled aggregate concrete under freeze–thaw cycles is established. The results show that under the action of freeze–thaw cycles, the capillary water absorption of recycled coarse aggregate concrete increases with the increase in the RCA substitution rate and decreases with the increase in nano-TiO2 content. After 150 freeze–thaw cycles, the cumulative water absorption and porosity of RC25-NT1.2 decreased by 25.52% and 14.57%, respectively, compared with the test block without nanomaterials. It was found that nano-TiO2 has a prominent role in modifying recycled aggregate concrete. Nano-TiO2 can reduce the cumulative water absorption and porosity of recycled aggregate concrete and alleviate the negative impact of the recycled coarse aggregate on capillary water absorption of concrete after freeze–thaw cycles. It was observed by scanning electron microscopy that a large amount of C–S–H gel was produced inside the concrete mixed with nano-TiO2, which bonded the internal pores and cracks to form a dense structure

Expression Dynamics of Core RNAi Machinery Genes in Pea Aphids Upon Exposure to Artificially Synthesized dsRNA and miRNAs

The pea aphid is an important pest of vegetables and causes serious losses worldwide. RNA interference (RNAi) is an effective pest control tool, and three sub-pathways have been described: The miRNA pathway, siRNA pathway, and piRNA pathway. A large number of genes in miRNA pathway and piRNA pathway are found to be expanded. To study the roles of these genes, the expression of 25 core RNAi genes was screened in spatiotemporal samples, artificially synthesized dsRNA and miRNA treated samples. The 25 genes were all expressed during different development stages and in different tissues. In dsRNA-treated samples and miRNA-treated samples, the expressions of genes in these three pathways were induced, especially the expanded genes. This suggests a complex network of RNAi core genes in the three sub-pathways. Treatment of miRNA seems to induce gene expression in a dosage-dependent manner. These results increase our knowledge of the siRNA pathway and related factors from RNAi pathway in aphids and promote the use of RNAi for the control of aphid pests

Environmental factors driving fungal distribution in freshwater lake sediments across the Headwater Region of the Yellow River, China

Dispersal limitation and environmental filtering are two primary processes involved in shaping microbial community structure. The pristine environmental and geographical relatively isolation of small lakes distributed in the Headwater Region of Yellow River (HRYR) offer a unique opportunity to test the relative roles of these two processes on fungal communities. Here, we investigated the fungal community in sediment samples from 10 lakes located in the HRYR using high-throughput sequencing. The results showed that the fungal community was dominated by Sordariomycetes, Leotiomycetes, Dothideomycetes, Pezizomycetes and Agaricomycetes. The results revealed that altitude, mean annual temperature, C/N ration, dissolve organic carbon and total nitrogen were the best predictors for shaping fungal community structure in these lakes. Significant spatial and environmental distance decay relationships in the fungal community were detected. The partial Mantel test indicated that the fungal community structure was significantly correlated with environmental distance but not with geographic distance. Overall, environmental filtering plays a more important role than dispersal limitation in fungal community structure at a local scale in such an pristine and isolated region

Characterizing the Proliferation Patterns of Representative Microsporidian Species Enlightens Future Studies of Infection Mechanisms

Background: Microsporidia are a group of pathogens that infect all kinds of animals, such as humans, silkworms, honeybees, and shrimp; they, therefore, pose a severe threat to public health and the economy. There are over 1500 species of microsporidia that have been reported, among which Encephalitozoon hellem and Nosema bombycis are the representative zoonotic and insect-infecting species, respectively. Investigating their cell infection patterns is of great significance for understanding their infection mechanisms. Methods: Specific probes were designed for the ribosomal RNA sequences of microsporidia. Fluorescence in situ hybridization (FISH) was used to trace the proliferation cycle of the pathogens in different cells. Results: Here, two rRNA large subunit gene (LSUrRNA) probes specifically labeling N. bombycis were obtained. The life cycle of N. bombycis in silkworm cells and E. hellem in three kinds of host cells was graphically drawn. N. bombycis meronts were first observed at 30 hours post-infection (hpi), and they began merogony. Sporonts were observed at 42 hpi, and the first entire proliferation cycle was completed at 48 hpi. The proliferation cycle of E. hellem in RK13 and HEK293 epithelial cells was almost the same, completing the first life cycle after 24 hpi, but it was significantly delayed to 32 hpi in RAW264.7. Conclusions: Specific FISH probes were established for labeling microsporidia in multiple host cells. The proliferation characteristics of representative zoonotic and insect-infecting microsporidian species were clarified. This study provides an experimental pattern for future analyses of microsporidian infection mechanisms

Characterization of the Largest Secretory Protein Family, Ricin B Lectin-like Protein, in Nosema bombycis: Insights into Microsporidian Adaptation to Host

Microsporidia are a group of obligate intracellular pathogens infecting nearly all animal phyla. The microsporidian Nosema bombycis has been isolated from several lepidopteran species, including the economy-important silkworms as well as several crop pests. Proteins secreted by parasites can be important virulent factors in modulating host pathways. Ricin is a two-chain lectin best known for its extreme vertebrate toxicity. Ricin B lectin-like proteins are widely distributed in microsporidia, especially in N. bombycis. In this study, we identify 52 Ricin B lectin-like proteins (RBLs) in N. bombycis. We show that the N. bombycis RBLs (NbRBLs) are classified into four subfamilies. The subfamily 1 was the most conserved, with all members having a Ricin B lectin domain and most members containing a signal peptide. The other three subfamilies were less conserved, and even lost the Ricin B lectin domain, suggesting that NbRBLs might be a multi-functional family. Our study here indicated that the NbRBL family had evolved by producing tandem duplications firstly and then expanded by segmental duplications, resulting in concentrated localizations mainly in three genomic regions. Moreover, based on RNA-seq data, we found that several Nbrbls were highly expressed during infection. Further, the results show that the NbRBL28 was secreted into host nucleus, where it promotes the expressions of genes involved in cell cycle progression. In summary, the great copy number, high divergence, and concentrated genome distribution of the NbRBLs demonstrated that these proteins might be adaptively evolved and played a vital role in the multi-host N. bombycis