Growth analysis of maize under waterlogging conditions

The objective of this work was to evaluate the influence of flooding on the growth of maize plants through growth analysis through simple logistics. The work was conducted in the 2016/2017 growing season. The climate is temperate, with well distributed rainfall, hot summer andthe soil is classified as solodiceutrophic haplic planosol. The experimental design was completely randomized blocks arranged in a factorial scheme, with two soil water conditions x five plant samples, arranged in four replicates. It was carried out the flooding of the parcels. The variables measured were total dry mass; dry matter production rates; relative growth rate; net assimilation rate; leaf area ratio; leaf mass ratio; maximum values of specific leaf area; leaf area index; efficiency of solar energy on version and mass partition, carried out in five collections. There is a reduction in leaf mass ratio, dry mass production rate, and relative growth rate in plants submitted to flooding, compared to those kept at field capacity. Corn plants submitted to soil flooding presented negative changes in the assimilated partition throughout their development. Soil flooding over the period of 72 hours adversely affects the physiological parameters of maize plants, leading to a reduction of their productive efficiency.The objective of this work was to evaluate the influence of flooding on the growth of maize plants through growth analysis through simple logistics. The work was conducted in the 2016/2017 growing season. The climate is temperate, with well distributed rainfall, hot summer andthe soil is classified as solodiceutrophic haplic planosol. The experimental design was completely randomized blocks arranged in a factorial scheme, with two soil water conditions x five plant samples, arranged in four replicates. It was carried out the flooding of the parcels. The variables measured were total dry mass; dry matter production rates; relative growth rate; net assimilation rate; leaf area ratio; leaf mass ratio; maximum values of specific leaf area; leaf area index; efficiency of solar energy on version and mass partition, carried out in five collections. There is a reduction in leaf mass ratio, dry mass production rate, and relative growth rate in plants submitted to flooding, compared to those kept at field capacity. Corn plants submitted to soil flooding presented negative changes in the assimilated partition throughout their development. Soil flooding over the period of 72 h adversely affects the physiological parameters of maize plants, leading to a reduction of their productive efficiency

Capacitação em “distúrbios de aprendizagem”

Trabalho apresentado no 31º SEURS - Seminário de Extensão Universitária da Região Sul, realizado em Florianópolis, SC, no período de 04 a 07 de agosto de 2013 - Universidade Federal de Santa Catarina.Nesse relato encontra-se o desenvolvimento de uma das atividades realizadas e experiências adquiridas no decorrer da Operação Capim Dourado realizada no município de Barrolândia – TO no ano de 2012, por meio do Projeto Rondon. A oficina de “Capacitação em distúrbios de aprendizagem” envolveu diversas secretarias do município e contou com a participação de professores e diretores das escolas locais. A experiência formou uma rede tecida por grandes fios de saberes experienciais, profissionais, curriculares e acima de tudo saberes pessoais que levaram a construção de cidadãos mais humanos a partir dessa experiência. Muitas vezes os professores não estão capacitados para diagnosticar crianças com esses distúrbios, e muito menos para aplicar métodos de ensino específicos para esses alunos. Diante desse contexto tem-se por objetivo empregar a mesma oficina realizada no Projeto Rondon, para capacitar professores e alunos da rede de ensino para a utilização de uma pluralidade metodológica que venha atender as particularidades existentes na sala de aula, bem como um olhar específico que deve ser voltado para os alunos que apresentam dificuldade e/ou distúrbios de aprendizagem

Analysis of seized stanozolol formulations in South Brazil by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry

Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone which are used medically for several diseases. However, misuse is commonly observed by athletes to promote enhancement of strength and performance. AAS are frequently obtained through online black markets from clandestine drug manufacturing laboratories, without any quality standards, being potentially dangerous for users. The purpose of this work was the development and application of a fast and simple procedure for the quantitation of stanozolol by liquid chromatography coupled to quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) in tablets packs seized in Rio Grande do Sul state, Brazil. The samples of stanozolol were separated considering its dosage form. The internal standard (methyltestosterone) was added to the aliquots of the samples, dissolved in methanol and 5μL were injected into the analytical system. The newly developed method has been validated for lower limit of quantitation (LLOQ), linearity, accuracy, precision and selectivity. The LLOQ was 0.1 µg/mL. The developed method was successfully applied to 31 samples seized by the Secretaria da Receita Federal do Brasil (a Brazilian federal revenue service agency). According to the results, 90.3% of the suspected medicines (n=31) were adulterated, and 65% exhibited higher concentrations of stanozolol than those indicated in the label. This work successfully established a new method for quantification of stanozolol using LC-QTOF-MS. This method aims at contributing to the identification and quantification of this anabolic androgenic steroid frequently seized by federal inspection agencies

TRILHA DOS SENTIDOS: ENTRELAÇANDO OS CAMINHOS DA CIÊNCIA E DA ARTE

Há um movimento mundial por uma Educação Ambiental mais estimuladora, propiciando o aumento de conhecimentos, mudanças de valores e o aperfeiçoamento de habilidades. O presente trabalho teve como objetivo analisar a realização da “Trilha dos Sentidos” (MATAREZI, 2006), com o intuito de promover nos participantes a conscientização de suas atitudes, posturas e seus quereres, bem como avaliar o desenvolvimento da atividade como um instrumento capaz de desenvolver habilidades na formação do ser humano criativo. Para tal, aplicamos a “Trilha dos Sentidos” durante a disciplina de Ciência e Arte, com os alunos do Programa de Pós-Graduação em Ensino em Biociências e Saúde (EBS/IOC/FIOCRUZ) e através de relatos avaliamos o potencial da atividade. Os resultados indicam que os participantes puderam experimentar o entrelaçamento da sensibilização e conscientização, além de despertar importantes habilidades para seu o desenvolvimento. Ademais, acreditamos que a atividade pode fomentar o diálogo entre as áreas de Ciências e Artes

Biochemical performance of bean seedlings under water restriction at the beginning of development

O presente trabalho teve como objetivo avaliar a o desempenho de cultivares de feijão submetidas ao déficit hídrico no desenvolvimento inicial das plantas. O experimento foi conduzido na Universidade Federal de Pelotas. As sementes utilizadas foram das cultivares BRS Esteio e IPR Tuiuiú, ambas cultivares do grupo preto. A restrição hídrica foi imposta utilizando PEG-6000 como redutor de potencial osmótico para realizar o teste de germinação. No décimo dia de germinação foram coletadas as plântulas para a avaliação dos seguintes testes: teores de prolina, peróxido de hidrogênio, peroxidação lipídica e enzimas antioxidante que foram avaliadas através da atividade específica das enzimas superóxido dismutase (SOD), catalase (CAT) e ascorbato peroxidase (APX). O delineamento utilizado foi de inteiramente casualizado, no esquema fatorial duas cultivares por quatro potenciais osmóticos. Os dados foram submetidos à análise de variância e os valores de F quando significativos foram comparados pelo teste de Tukey a 5% de probabilidade. Os resultados demonstram que a restrição hídrica, conforme o potencial osmótico, afeta de forma distinta a atividade das enzimas CAT, SOD, APX. O que pode resultar em plantas menos adaptadas ao estresse hídrico.The present work aimed to evaluate the performance of bean cultivars submitted to water deficit in the initial development of plants. The experiment was conducted at the Federal University of Pelotas. The seeds used were from the cultivars BRS Esteio and IPR Tuiuú, both cultivars from the black group. Water restriction was imposed using PEG-6000 as an osmotic potential reducer to perform the germination test. On the tenth day of germination, seedlings were collected for the evaluation of the following tests: levels of proline, hydrogen peroxide, lipid peroxidation and antioxidant enzymes that were evaluated through the specific activity of the enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The design used was completely randomized, in the factorial scheme two cultivars for four osmotic potentials. The data were subjected to analysis of variance and the F values when significant were compared using the Tukey test at 5% probability. The results demonstrate that water restriction, according to the osmotic potential, affects CAT, SOD, APX enzymes in a different way. Which can result in plants less adapted to water stress.Facultad de Ciencias Agrarias y Forestale

Desempenho bioquímico de plântulas de feijão sob restrição hídrica no início do desenvolvimento

The present work aimed to evaluate the performance of bean cultivars submitted to water deficit in the initial development of plants. The experiment was conducted at the Federal University of Pelotas. The seeds used were from the cultivars BRS Esteio and IPR Tuiuú, both cultivars from the black group. Water restriction was imposed using PEG-6000 as an osmotic potential reducer to perform the germination test. On the tenth day of germination, seedlings were collected for the evaluation of the following tests: levels of proline, hydrogen peroxide, lipid peroxidation and antioxidant enzymes that were evaluated through the specific activity of the enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The design used was completely randomized, in the factorial scheme two cultivars for four osmotic potentials. The data were subjected to analysis of variance and the F values ​​when significant were compared using the Tukey test at 5% probability. The results demonstrate that water restriction, according to the osmotic potential, affects CAT, SOD, APX enzymes in a different way. Which can result in plants less adapted to water stress.O presente trabalho teve como objetivo avaliar a o desempenho de cultivares de feijão submetidas ao déficit hídrico no desenvolvimento inicial das plantas. O experimento foi conduzido na Universidade Federal de Pelotas. As sementes utilizadas foram das cultivares BRS Esteio e IPR Tuiuiú, ambas cultivares do grupo preto. A restrição hídrica foi imposta utilizando PEG-6000 como redutor de potencial osmótico para realizar o teste de germinação. No décimo dia de germinação foram coletadas as plântulas para a avaliação dos seguintes testes: teores de prolina, peróxido de hidrogênio, peroxidação lipídica e enzimas antioxidante que foram avaliadas através da atividade específica das enzimas superóxido dismutase (SOD), catalase (CAT) e ascorbato peroxidase (APX). O delineamento utilizado foi de inteiramente casualizado, no esquema fatorial duas cultivares por quatro potenciais osmóticos. Os dados foram submetidos à análise de variância e os valores de F quando significativos foram comparados pelo teste de Tukey a 5% de probabilidade. Os resultados demonstram que a restrição hídrica, conforme o potencial osmótico, afeta de forma distinta a atividade das enzimas CAT, SOD, APX. O que pode resultar em plantas menos adaptadas ao estresse hídrico

Defoliation intensity and performance of soybean plants with different growth habits

The objective of this work was to evaluate the influence of the level of artificial defoliation, applied in stages V6 and R2, on the quality and yield components of soybean seeds. The experiment was conducted in a greenhouse in the municipality of Cascavel, Paraná State, Brazil. The experimental design was a randomized block with four replications, in a 5 x 2 x 2 factorial scheme (five levels of defoliation x two cultivars, two phenological stages (V6: fifth fully developed trifoliolate leaf and R2: full flowering), two growth habits). The analyzed variables were mass of 100 seeds, number of pods per plant, seed number per plant, seed number per pod, germination, dry matter of seedlings and length of shoot and roots of seedlings. Defoliation above 50% results in the reduction of the number of pods and seeds per plant to grow indeterminate growth and reduction in the mass of 100 seeds for cultivars of determined growth

SOX10 requirement for melanoma tumor growth is due, in part, to immune-mediated effects

Developmental factors may regulate the expression of immune modulatory proteins in cancer, linking embryonic development and cancer cell immune evasion. This is particularly relevant in melanoma because immune checkpoint inhibitors are commonly used in the clinic. SRY-box transcription factor 10 (SOX10) mediates neural crest development and is required for melanoma cell growth. In this study, we investigate immune-related targets of SOX10 and observe positive regulation of herpesvirus entry mediator (HVEM) and carcinoembryonic-antigen cell-adhesion molecule 1 (CEACAM1). Sox10 knockout reduces tumor growth in vivo, and this effect is exacerbated in immune-competent models. Modulation of CEACAM1 expression but not HVEM elicits modest effects on tumor growth. Importantly, Sox10 knockout effects on tumor growth are dependent, in part, on CD8+ T cells. Extending this analysis to samples from patients with cutaneous melanoma, we observe a negative correlation with SOX10 and immune-related pathways. These data demonstrate a role for SOX10 in regulating immune checkpoint protein expression and anti-tumor immunity in melanoma

TAMANHO DA SEMENTE, VIGOR E CRESCIMENTO INICIAL DE TREVO ENCARNADO

O objetivo do trabalho foi avaliar o efeito do tamanho da semente no vigor e no crescimento inicial de plantas de trevo encarnado. Foram utilizadas sementes da cultivar Crimson Clover e os tratamentos se constituíram em três tamanhos de sementes (grandes - comprimento 2,33; largura 1,63; espessura 1,35 mm; médias - comprimento 2,65; largura 2,06; espessura 1,75; pequenas - comprimento 2,33; largura 1,63; espessura 1,35 mm). Para a avaliação do efeito do tamanho da semente sobre o potencial fisiológico das sementes foram efetuados os seguintes testes: germinação, primeira contagem, emergência a campo, índice de velocidade de emergência, área foliar, matéria seca da parte aérea e da raiz, condutividade elétrica e índice de clorofila. O tamanho das sementes não influencia na germinação, emergência e primeira contagem de germinação. A área foliar apresentou interação entre os tamanhos analisados, a parte aérea e a massa seca da parte aérea apresentaram efeito significativo para os tamanhos de sementes. A área foliar, o comprimento da parte aérea e a massa seca de parte aérea apresentam maiores valores quando originaram-se de sementes grandes. Portanto, sementes grandes, de trevo encarnado, propiciaram melhor desempenho em campo