Earthquakes and slip rate of the southern Sagaing fault: insights from an offset ancient fort wall, lower Burma (Myanmar)

Field investigations of an ancient fortress wall in southern Myanmar reveal an offset of ~6 m across the Sagaing fault, the major right-lateral fault between the Sunda and Burma plates. The fault slip rate implied by offset of this 16th-century fortress is between 11 and 18 cm yr^(–1). A palaeoseismological excavation within the fortress reveals at least two major fault ruptures since its construction. The slip rate we obtained is comparable to geodetic and geological estimates farther north, but is only 50 per cent of the spreading rate (38 mm yr^(–1)) at the Andaman Sea spreading centre. This disparity suggests that other structures may be accommodating deformation within the Burma Plate. We propose two fault-slip scenarios to explain the earthquake-rupture history of the southern Sagaing fault. Using both small offset features along the fault trace and historical records, we speculate that the southern Sagaing fault exhibits a uniform-fault-slip behaviour and that one section of the fault could generate a M7+ earthquake within the next few decade

Evaluation on dry forage yields and nutritional characteristics of introduced herbaceous legumes in Myanmar

The study was carried out to evaluate the forage yields, nutritive values and in vitro fermentation parameters of herbaceous legumes. Five varieties of introduced herbaceous legumes; Stylosanthes guianensis cv. Ubon stylo, Macrotyloma axillare cv. Archer, Centrosema brasilianum cv. Ooloo, Stylosanthes guianensis cv. Stylo 184 and Macroptilum bracteatum cv. Cadarga were evaluated at the research farm, University of Veterinary Science, Yezin, Myanmar. No fertilizer and no irrigation were applied for cultivation to test drought resistance. Dry forage yield, nutritive values and gas production at four harvesting times were measured with 4×5 factorial arrangement (5 legumes and 4 harvesting time) in randomized complete block design. There was no interaction between legumes and harvesting time on forage yield, nutritive values and fermentation parameters but they were affected by the main effects of legume types and harvesting time. Among the legume forages, the highest dry forage yields were found in Ooloo, Ubon stylo, and Stylo 184, and followed by the DM yield of Archer and Cadarga. The DM yield of the second harvest was significantly higher (p<0.05) than those of the first, third and fourth harvest which were not significantly different from each other. As a chemical composition, the DM content of Archer was lower (p<0.05) than those of other varieties. Among the legumes forages, the lower CP content was found in Cadarga. The higher NDF was observed in Ooloo. Ooloo, Ubon stylo and Cadarga showed higher ADF in comparison with the other two varieties. Among the harvesting time, the lowest DM content was found at the first harvest. The highest CP content was found at third harvest. The NDF content was not significantly different. The lowest ADF content was found in fourth harvest. According to the dry forage yield, Ubon stylo and Ooloo had the highest dry forage yield and in term of nutritive values, Stylo 184 and Archer had higher nutritive values. As the main effect of forages, Stylo 184 and Archer had higher gas production in comparison with the other varieties. As the main effect of harvesting time, the fourth harvest had the highest gas production in comparison with other harvesting time. It could be better for cultivation by application of fertilizer and irrigation to get more forage yield and quality. &nbsp

PRL3-zumab, a first-in-class humanized antibody for cancer therapy

Novel, tumor-specific drugs are urgently needed for a breakthrough in cancer therapy. Herein, we generated a first-in-class humanized antibody (PRL3-zumab) against PRL-3, an intracellular tumor-associated phosphatase upregulated in multiple human cancers, for unconventional cancer immunotherapies. We focused on gastric cancer (GC), wherein elevated PRL-3 mRNA levels significantly correlated with shortened overall survival of GC patients. PRL-3 protein was overexpressed in 85% of fresh-frozen clinical gastric tumor samples examined but not in patient-matched normal gastric tissues. Using human GC cell lines, we demonstrated that PRL3-zumab specifically blocked PRL-3(+), but not PRL-3(–), orthotopic gastric tumors. In this setting, PRL3-zumab had better therapeutic efficacy as a monotherapy, rather than simultaneous combination with 5-fluorouracil or 5-fluorouracil alone. PRL3-zumab could also prevent PRL-3(+) tumor recurrence. Mechanistically, we found that intracellular PRL-3 antigens could be externalized to become “extracellular oncotargets” that serve as bait for PRL3-zumab binding to potentially bridge and recruit immunocytes into tumor microenvironments for killing effects on cancer cells. In summary, our results document a comprehensive cancer therapeutic approach to specific antibody-targeted therapy against the PRL-3 oncotarget as a case study for developing antibodies against other intracellular targets in drug discovery

Confirmation of Skywalker Hoolock Gibbon (Hoolock tianxing) in Myanmar extends known geographic range of an endangered primate

Characterizing genetically distinct populations of primates is important for protecting biodiversity and effectively allocating conservation resources. Skywalker gibbons (Hoolock tianxing) were first described in 2017, with the only confirmed population consisting of 150 individuals in Mt. Gaoligong, Yunnan Province, China. Based on river geography, the distribution of the skywalker gibbon has been hypothesized to extend into Myanmar between the N’Mai Kha and Ayeyarwaddy Rivers to the west, and the Salween River (named the Thanlwin River in Myanmar and Nujiang River in China) to the east. We conducted acoustic point-count sampling surveys, collected noninvasive samples for molecular mitochondrial cytochrome b gene identification, and took photographs for morphological identification at six sites in Kachin State and three sites in Shan State to determine the presence of skywalker gibbons in predicted suitable forest areas in Myanmar. We also conducted 50 semistructured interviews with members of communities surrounding gibbon range forests to understand potential threats. In Kachin State, we audio-recorded 23 gibbon groups with group densities ranging between 0.57 and 3.6 group/km2. In Shan State, we audio-recorded 21 gibbon groups with group densities ranging between 0.134 and 1.0 group/km2. Based on genetic data obtained from skin and saliva samples, the gibbons were identified as skywalker gibbons (99.54–100% identity). Although these findings increase the species’ known population size and confirmed distribution, skywalker gibbons in Myanmar are threatened by local habitat loss, degradation, and fragmentation. Most of the skywalker gibbon population in Myanmar exists outside protected areas. Therefore, the IUCN Red List status of the skywalker gibbon should remain as Endangered

Evidence of Quaternary and recent activity along the Kyaukkyan Fault, Myanmar

Cenozoic right-lateral shear between the eastern Indian margin and Eurasia is expressed by numerous N-S trending fault systems inboard of the Sunda trench, including the Sagaing Fault. The most easterly of these fault systems is the prominent ∼500 km long Kyaukkyan Fault, on the Shan Plateau. Myanmar’s largest recorded earthquake, Mw 7.7 on 23rd May 1912, focused near Maymyo, has been attributed to the Kyaukkyan Fault, but the area has experienced little significant seismicity since then. Despite its demonstrated seismic potential and remarkable topographic expression, questions remain about the Kyaukkyan Fault’s neotectonic history. In this study we document robust geologic evidence of fault activity along the Kyaukkyan Fault. Field investigation and interpretation of satellite imagery reveal deformation features developed along a fault system mostly indicative of Quaternary dextral strike-slip faulting. Clearly defined tectonic geomorphology, including fault scarps and linear valleys, are used to trace the northernmost and southernmost part of the fault. The fault’s central section is characterised by a complex pull-apart system, whose normal border faults show signals of relatively slow recent activity. Dextral transtensional activity along the Kyaukkyan Fault is recorded by geomorphic features such as sag ponds, shutter ridges, offset and beheaded streams, triangular facets and low-sinuosity mountain fronts. The Quaternary age of activity is demonstrated by short-lived geomorphic features such as wind-gaps, offset and deformed alluvial fans, and even offset of man-made structures. In the Inle basin, alluvial fan successions along the easternmost mountain front reveal a vertical transition from faulted and folded alluvial fan sequences adjacent to pre-Cenozoic flanking ranges, to overlying gravels that appear less deformed. Conversely, a locally buried cross-basin fault system has fresh geomorphic expression even within the uppermost parts of the lacustrine/fluvial basin fill. This may indicate Quaternary migration of dominant fault deformation from sidewall faults to a cross-basin fault system, which is indicative of a mature strike-slip fault system and has been observed in other active strike-slip faults around the world and in analogue models.Published207-2252TR. Ricostruzione e modellazione della struttura crostaleJCR Journa

The Cholinergic Pathway and MitoKATP Induce UCP4 Expression Involved in Neuroprotection of FN Stimulation in Rats

Background Electrical stimulation of the cerebellar fastigial nucleus (FN) reduces the infarct size induced by middle cerebral artery occlusion in rats. FN stimulation confers long‐lasting protection from brain injury; however, its underlying mechanism is not yet understood. We aimed to elucidate the mechanism by which FN stimulation exerts neuroprotection. We hypothesized that the neuroprotective effect of FN stimulation involves activation of cholinergic pathways, which increases reactive oxygen species (ROS) production by opening mitochondrial K+ATP channels, thus leading to an increase in UCP4 (uncoupling protein 4) expression and subsequent neuroprotection. Methods FN stimulation was performed for 1 hour in rats. The UCP4 protein and mRNA levels were measured by western blot, dot blot, and in situ hybridization. Carbachol was applied following UCP4‐promoter tdTomato reporter vector transfection of the rat primary cortical cell culture (in vitro) and rat brain (in vivo). We observed cellular UCP4 expression using fluorescence microscopy. UCP4 expression in the cell culture in response to diazoxide application was determined by a reverse transcription‐polymerase chain reaction and western blotting. Results Whereas FN stimulation increased UCP4 protein and mRNA levels, carbachol administration induced UCP4 expression in vitro and in vivo. The attenuation of this effect by atropine suggests that FN‐induced UCP4 expression involves the cholinergic pathway. The opening of mitochondrial K+ATP channels with diazoxide increased the production of ROS and led to increased UCP4 expression. In contrast, quenching ROS with superoxide dismutase reversed the effect of diazoxide on UCP4 expression. Therefore, the opening of mitochondrial K+ATP channels increased ROS production, which subsequently enhanced UCP4 expression and attenuated ROS generation. Conclusion Neuroprotective effect of FN stimulation involves activation of the cholinergic pathways, which increases ROS production by opening mitochondrial K+ATP channels, leading to increased expression of neuroprotective UCP4

Permanent upper plate deformation in western Myanmar during the great 1762 earthquake : implications for neotectonic behavior of the northern Sunda megathrust

The 1762 Arakan earthquake resulted from rupture of the northern Sunda megathrust and is one of those rare preinstrumental earthquakes for which early historical accounts document ground deformations. In order to obtain more comprehensive and detailed measurements of coseismic uplift, we conducted comprehensive field investigations and geochronological analyses of marine terraces on the two largest islands in western Myanmar. We confirm 3–4 m of coseismic coastal emergence along southwestern Cheduba Island, diminishing northeastward to less than 1 m. Farther northeast, uplift associated with the earthquake ranges from slightly more than 1 m to 5–6 m along the western coast of Ramree Island but is insignificant along the island’s eastern coast. This double-hump pattern of uplift coincides with the long-term anticlinal growth of these two islands. Thus, we propose that the 1762 earthquake resulted from slip on splay faults under the islands, in addition to rupture of the megathrust. Elastic modeling implies that fault slip during the 1762 earthquake ranges from about 9 to 16 m beneath the islands and corresponds to a magnitude of Mw 8.5 if the rupture length of the megathrust is ~500 km. The island’s uplift histories suggest recurrence intervals of such events of about 500–700 years. Additional detailed paleoseismological studies would add significant additional detail to the history of large earthquakes in this region.Published versio

Surface ruptures of the Mw 6.8 March 2011 Tarlay earthquake, Eastern Myanmar

Field observations indicate the Mw 6.8 Tarlay, Myanmar, earthquake of 24 March 2011 resulted from the rupture of a short section of the left‐lateral Nam Ma fault. We document coseismic left‐lateral offsets that range from approximately 10 cm to about 2 m along a 19 km long section of the fault, based upon both field investigations and the interpretation of high‐resolution satellite imagery. Comparison of these early field and later remote measurements suggests that most of the paddy‐field offsets became obscured within a year or two. Our measurements indicate the magnitude of sinistral offset decreases gradually eastward from the epicentral area to the rupture terminus within the Tarlay basin, along the southern edge of a 2–3 km wide transtensional stepover. Our measurements confirm that the earthquake resulted from rupture of a structurally distinct segment of the Nam Ma fault, bounded on the west by the fault’s terminus and on the east by the Tarlay basin stepover. If the 215 km Nam Ma fault were to rupture entirely in a single event, the magnitude of the resulting earthquake would likely be about Mw 7.7