Development of a Temperature Measurement Technique Using Molecular Tagging Velocimetry

A summary of the development of a method of measuring fluid temperature with a laser is presented. The method is based on molecular tagging velocimetry (MTV), an established technique of obtaining spatially-resolved fluid velocity profiles by analysis of laser-induced molecular phosphorescence emitted by photoluminescent chemicals. In aqueous solutions the intensity of the phosphorescent emission is inversely related to the temperature of the fluid. Research into quantifying the relationship between the solution temperature and emission intensity has resulted in the development of a calibration curve which may be used for subsequent temperature measurements. Error analysis shows that temperatures may be determined over a range of nearly 30oC with a typical error of less than +/- 2.66% or +/- 0.8oC, with a 95% confidence level. A discussion of the technique development, including experimental methods, calibration curve development, and error analysis, is here presented

High-Speed, Three-Dimensional Quantification of Ladybug Flapping Wing Kinematics During Takeoff

Ladybug wing and body kinematics during takeoff is explored using high-speed stereoscopic images acquired at a rate of 3000 frames per second. A direct linear transformation algorithm is used to quantify positions of selected locations on the body, forewings (elytra), and hindwings. Design and setup of instrumentation and analysis procedures are explained. Flapping frequency is reported. Significant motion of the forewing and other findings are presented and their applications are discussed

Design of a Flapping Wing Mechanism for Force Analysis and Optimization

The design of a robotic flapping wing mechanism is discussed. The design allows for dynamic adjustment of flapping trajectory in fluid with three rotational degrees of freedom, while keeping all motors and encoders out of the fluid (i.e., water or oil) to protect critical equipment from potential failure and increase reliability. Mechanism control is discussed. Preliminary optimization using a Box-Behnkin design approach is used and shows successful parameter optimization. Mechanism limitations are addressed

Navigating the challenges of digital health innovation: considerations and solutions in developing online and smartphone application based interventions for mental health disorders

This article presents an analysis of challenges and considerations when developing digital mental health innovations. Recommendations include collaborative working between clinicians, researchers, industry and service users in order to successfully navigate challenges and to ensure e-therapies are engaging, acceptable, evidence based, scalable and sustainable

Whole-field 3D Characterization of a Pulsating Jet using Synthetic Aperture Particle Image Velocimety

In this study synthetic aperture particle image velocimetry is used on an excised human vocal fold model to study the airflow over the vocal folds during voice production. For the first time, a whole-field, time-resolved, 3D description of the flow is presented over one cycle of vocal fold oscillation. Complex, unsteady, 3D flow behavior is observed as the jet evolves

The phase diagrams of KCaF3 and NaMgF3 by ab initio simulations

ABF3 compounds have been found to make valuable low-pressure analogues for high-pressure silicate phases that are present in the Earth’s deep interior and that may also occur in the interiors of exoplanets. The phase diagrams of two of these materials, KCaF3 and NaMgF3, have been investigated in detail by static ab initio computer simulations based on density functional theory. Six ABF3 polymorphs were considered, as follows: the orthorhombic perovskite structure (GdFeO3-type; space group Pbnm); the orthorhombic CaIrO3 structure (Cmcm; commonly referred to as the “post-perovskite” structure); the orthorhombic Sb2S3 and La2S3 structures (both Pmcn); the hexagonal structure previously suggested in computer simulations of NaMgF3 (P63/mmc); the monoclinic structure found to be intermediate between the perovskite and CaIrO3 structures in CaRhO3 (P21/m). Volumetric and axial equations of state of all phases considered are presented. For KCaF3, as expected, the perovskite phase is shown to be the most thermodynamically stable at atmospheric pressure. With increasing pressure, the relative stability of the KCaF3 phases then follows the sequence: perovskite → La2S3 structure → Sb2S3 structure → P63/mmc structure; the CaIrO3 structure is never the most stable form. Above about 2.6 GPa, however, none of the KCaF3 polymorphs are stable with respect to dissociation into KF and CaF2. The possibility that high-pressure KCaF3 polymorphs might exist metastably at 300 K, or might be stabilised by chemical substitution so as to occur within the standard operating range of a multi-anvil press, is briefly discussed. For NaMgF3, the transitions to the high-pressure phases occur at pressures outside the normal range of a multi-anvil press. Two different sequences of transitions had previously been suggested from computer simulations. With increasing pressure, we find that the relative stability of the NaMgF3 phases follows the sequence: perovskite → CaIrO3 structure → Sb2S3 structure → P63/mmc structure. However, only the perovskite and CaIrO3 structures are stable with respect to dissociation into NaF and MgF2

Toxoplasma gondii Infection Specifically Increases the Levels of Key Host MicroRNAs

The apicomplexan parasite Toxoplasma gondii can infect and replicate in virtually any nucleated cell in many species of warm-blooded animals; thus, it has evolved the ability to exploit well-conserved biological processes common to its diverse hosts. Here we have investigated whether Toxoplasma modulates the levels of host microRNAs (miRNAs) during infection.Using microarray profiling and a combination of conventional molecular approaches we report that Toxoplasma specifically modulates the expression of important host microRNAs during infection. We show that both the primary transcripts for miR-17 approximately 92 and miR-106b approximately 25 and the pivotal miRNAs that are derived from miR-17 approximately 92 display increased abundance in Toxoplasma-infected primary human cells; a Toxoplasma-dependent up-regulation of the miR-17 approximately 92 promoter is at least partly responsible for this increase. The abundance of mature miR-17 family members, which are derived from these two miRNA clusters, remains unchanged in host cells infected with the closely related apicomplexan Neospora caninum; thus, the Toxoplasma-induced increase in their abundance is a highly directed process rather than a general host response to infection.Altered levels of miR-17 approximately 92 and miR-106b approximately 25 are known to play crucial roles in mammalian cell regulation and have been implicated in numerous hyperproliferative diseases although the mechanisms driving their altered expression are unknown. Hence, in addition to the implications of these findings on the host-pathogen interaction, Toxoplasma may represent a powerful probe for understanding the normal mechanisms that regulate the levels of key host miRNAs

Taxonomy Based on Science Is Necessary for Global Conservation [Formal comment]

Taxonomy is a scientific discipline that has provided the universal naming and classification system of biodiversity for centuries and continues effectively to accommodate new knowledge. A recent publication by Garnett and Christidis expressed concerns regarding the difficulty that taxonomic changes represent for conservation efforts and proposed the establishment of a system to govern taxonomic changes. Their proposal to “restrict the freedom of taxonomic action” through governing subcommittees that would “review taxonomic papers for compliance” and their assertion that “the scientific community\u27s failure to govern taxonomy threatens the effectiveness of global efforts to halt biodiversity loss, damages the credibility of science, and is expensive to society” are flawed in many respects. They also assert that the lack of governance of taxonomy damages conservation efforts, harms the credibility of science, and is costly to society. Despite its fairly recent release, Garnett and Christidis\u27 proposition has already been rejected by a number of colleagues. Herein, we contribute to the conversation between taxonomists and conservation biologists aiming to clarify some misunderstandings and issues in the proposition by Garnett and Christidis. Placing governance over the science of taxonomy blurs the distinction between taxonomy and nomenclature. Garnett and Christidis\u27s proposal is far-reaching but represents a narrow perspective of taxonomy, as utilized by conservation, and reflects an increasingly broad misunderstanding throughout biology of the scientific basis of taxonomy, formalized nomenclature, and the relationship between them. This trend may have resulted from the attenuation of instruction in taxonomic principles and, in particular, nomenclature at many universities, in part because of a shift in research priorities away from taxonomy