Syntax-based skill extractor for job advertisements

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Costs, revenues and simulated consolidation of selected Missouri counties

"Publication authorized March 6, 1969

Structural overview of the Missouri economy in 1990

Cover title.At head of title: "A Special Report.""SR485 New 4/94/1.5M.

Advances in the design of macroporous polymer scaffolds for potential applications in dentistry

A paradigm shift is taking place in medicine and dentistry from using synthetic implants and tissue grafts to a tissue engineering approach that uses degradable porous three-dimensional (3D) material hydrogels integrated with cells and bioactive factors to regenerate tissues such as dental bone and other oral tissues. Hydrogels have been established as a biomaterial of choice for many years, as they offer diverse properties that make them ideal in regenerative medicine, including dental applications. Being highly biocompatible and similar to native extracellular matrix, hydrogels have emerged as ideal candidates in the design of 3D scaffolds for tissue regeneration and drug delivery applications. However, precise control over hydrogel properties, such as porosity, pore size, and pore interconnectivity, remains a challenge. Traditional techniques for creating conventional crosslinked polymers have demonstrated limited success in the formation of hydrogels with large pore size, thus limiting cellular infiltration, tissue ingrowth, vascularization, and matrix mineralization (in the case of bone) of tissue-engineered constructs. Emerging technologies have demonstrated the ability to control microarchitectural features in hydrogels such as the creation of large pore size, porosity, and pore interconnectivity, thus allowing the creation of engineered hydrogel scaffolds with a structure and function closely mimicking native tissues. In this review, we explore the various technologies available for the preparation of macroporous scaffolds and their potential applications

Skisturz mit temporärer Tetraparese

Zusammenfassung. Wir präsentieren einen 74-jährigen Patienten nach Skisturz und danach aufgetretener inkompletter, armbetonter sensibler und motorisch spastischer Tetraparese. Bei Vorstellung in der Hausarztpraxis konnten sowohl Pyramidenbahnzeichen sowie Störungen der Feinmotorik in beiden Händen beobachtet werden. Zudem zeigte sich ein sensibles und ataktisches Gangbild. Klinisch zeigte sich zusammenfassend eine traumatische inkomplette sensible und motorische Querschnittslähmung NLI C5 und AIS D. Die MRT-Untersuchung der Halswirbelsäule zeigte passend hierzu eine hochgradige Spinalkanalstenose auf Höhe C5 mit Myelonkompression. Es erfolgte eine operative Dekompression des Rückenmarks mit anschliessender Fusion und Stabilisierung der entsprechenden Halswirbelkörper. Postoperativ zeigte sich klinisch weiterhin eine Querschnittslähmung NLI C5 und AIS D. Nach dreiwöchiger neurologischer Rehabilitation hat sich der Patient bis auf eine leichte Paraspastik der Beine und noch leicht bestehenden Hypästhesien der Fingerbeeren erholt. Skiing Accident with Temporary Tetraparesis Abstract. Summary: We present the case of a 74-year-old patient who initially suffered transient tetraplegia after a skiing accident. On presentation to the general practitioner, pyramidal tract signs as well as disturbances of fine motor function in both hands could be observed. MRI examinations of the cervical spine revealed high-grade spinal stenosis at level C5 with myelon compression. Surgical decompression of the spial cord, followed by fusion of the corresponding cervical vertebral bodies, was performed. After surgery and three weeks of neurological rehabilitation, the patient feels well and has recovered except for still existing hypesthesia of the fingertips

A unified approach to dielectric single cell analysis: Impedance and dielectrophoretic force spectroscopy

In this review we present a unified approach for single cell dielectric spectroscopy. Impedance spectroscopy and dielectrophoretic cell sorting, current microtechnologies applied in electrical analysis of single cells are discussed based on their closely related physical principles. In addition, examples of microfluidic devices will be presented: a microfabricated flow cytometer for single cell discrimination based on impedance analysis and a miniaturized continuous dielectrophoretic cell sorter, both using the concept of liquid electrodes. Using the experimental results obtained from both microdevices, we give a comparative overview over the dielectrophoretic sorting and impedance spectroscopy

Characterization of the erythrocyte response to glucose levels by impedance spectroscopy

[...] For many years, researchers in the biosensors field have been trying to develop a device allowing the precise measurement of the glucose level through the intact skin. In this project, an efficient device was constructed for the in-vitro impedance measurement in order to see the frequential response of RBC, the beta-dispersion. [... click on "Download fulltext" for full abstract

Fluidic microstructuring of alginate hydrogels for the single cell niche

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main approach today is the use of metabolic agents that block the cell cycle at a particular phase and accumulate cells at this phase, disturbing the cell physiology. We provide here a non-invasive and label-free continuous cell sorting technique to analyze and synchronize yeast cell division. By balancing opposing dielectrophoretic forces at multiple frequencies, we maximize sensitivity to the characteristic shape and internal structure changes occurring during the yeast cell cycle, allowing us to synchronize the culture in late anaphase

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on microfabricated flow cytometer

Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The cellular modifications caused by the intracellular parasite result in a shift in impedance which can be measured dielectrically. Thus, a rapid cell-by-cell detection with microliter amounts of reagents is possible. Unlike other diagnostic tests, this method does not depend on extensive sample pre-treatment or expensive chemicals and equipment