THIRD INTERNATIONAL SYMPOSIUM ON RANAVIRUSES:: ADVANCING THE UNDERSTANDING OF THE THREAT OF RANAVIRUSES TO NORTH AMERICAN HERPETOFAUNA

Members of the genus Ranavirus, one of five genera withinthe family Iridoviridae, encompass a group of large, doublestrandedDNA viruses that infect all three classes of ectothermicvertebrates: fish, amphibians, and reptiles. Ranaviruses areglobally emerging pathogens that cause considerable morbidityand mortality among diverse populations. In North America,ranavirus epizootics are regularly reported in wild and culturedfish, amphibian, and reptile populations

Genome sequence of a novel alloherpesvirus isolated from glass catfish (Kryptopterus bicirrhis)

The 149,343-bp genome of silurid herpesvirus 1, which was isolated in Thailand from glass catfish, was sequenced. The genome was most closely related to that of ictalurid herpesvirus 2, which infects black bullhead catfish. To our knowledge, this was the first silurid catfish alloherpesvirus genome to be sequenced

Characterisation and comparison of the mucosa-associated bacterial communities across the gastrointestinal tract of stranded green turtles, Chelonia mydas

Chelonia mydas are primarily herbivorous long-distance migratory sea turtles that contribute to marine ecosystems. Extensive research has been conducted to restore the populations of green turtles. Little is known about their gut microbiota which plays a vital role in their health. We investigated the mucosa-associated bacterial communities across the gastrointestinal (GI) tract of a total four (3, juvenile and 1, adult) stranded green turtles. Samples taken from four GI regions including oesophagus, stomach, small intestine and large intestine were analysed by high-throughput sequencing targeting hypervariable V1-V3 regions of the bacterial 16S rRNA gene. Bacterial diversity and richness decreased longitudinally along the GI tract from oesophagus to the small intestine of stranded turtles. The large intestine showed a higher bacterial diversity and richness compared to small intestine. The bacterial community of green turtles’ GI tract was largely dominated by Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes and Fusobacteria. Aerobic and facultative anaerobic bacteria prevailed primarily in the oesophagus while anaerobes (Lachnospiraceae, Peptostreptococcaceae and Ruminococcaceae) constituted the bulk of large intestinal microbiota. Firmicutes dominated the GI tract except within the small intestine where Proteobacteria prevailed. At the OTU level, six percent of the total OTUs (>1% relative abundance) were common in all GI regions. This is a comprehensive characterisation of bacterial microbiota across the GI tract in green turtles which will provide a reference for future studies on turtle gut microbiome and their metabolism to improve their health and nutrition during rehabilitation

Genome sequence of a gammaherpesvirus from a common bottlenose dolphin (Tursiops truncatus)

A herpesvirus genome was sequenced directly from a biopsy specimen of a rectal lesion from a female common bottlenose dolphin. This genome sequence comprises a unique region (161,235 bp) flanked by multiple copies of a terminal repeat (4,431 bp) and contains 72 putative genes. The virus was named common bottlenose dolphin gammaherpesvirus 1

Genomic Sequencing of Ranaviruses Isolated from Turbot (Scophthalmus maximus) and Atlantic Cod (Gadus morhua)

Ranaviruses have been isolated from Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus) in Denmark. Phylogenomic analyses revealed that these two ranaviruses are nearly identical and form a distinct clade at the base of the ranavirus tree branching off near other fish ranaviruses

Genome sequence of an alphaherpesvirus from a beluga whale (Delphinapterus leucas)

Beluga whale alphaherpesvirus 1 was isolated from a blowhole swab taken from a juvenile beluga whale. The genome is 144,144 bp in size and contains 86 putative genes. The virus groups phylogenetically with members of the genus Varicellovirus in subfamily Alphaherpesvirinae and is the first alphaherpesvirus sequenced from a marine mammal

Atlantic salmon papillomatosis in Russia and molecular characterization of the associated herpesvirus.

Papillomatosis of Atlantic salmon Salmo salar has been reported for decades in Russia, Scandinavia and Scotland. The disease is typically benign although heavy losses have occasionally been reported. A herpesviral etiology has been suggested based on ultrastructural evidence; however, the virus has not been isolated or genetically characterized. In this study, we provide the first viral sequences detected in the papillomas from diseased Russian Atlantic salmon. Phylogenetic analyses, based on the partial sequences of the herpesviral polymerase and terminase genes, supported the virus as a novel member of the genus Salmonivirus within the family Alloherpesviridae. The sequences of the Atlantic salmon papillomatosis virus differ markedly from those of the 3 known salmoniviruses; therefore, the authors propose the species designation Salmonid herpesvirus 4 to be considered for approval by the International Committee on Taxonomy of Viruses

Genomic sequence of a ranavirus isolated from pike-perch Sander lucioperca

The pike-perch iridovirus (PPIV) was isolated in Finland from apparently healthy pike-perch fingerlings during routine disease surveillance. Our phylogenomic analysis revealed that PPIV is the first fish member of a clade of ranaviruses previously described from European and Chinese amphibians