Content Analysis: U.S. Newspaper and social media portrayal of President Obama in association with the killing of Osama bin Laden during the 2012 presidential election year

In a broad sense, public relations is the communication between an organization and its audience. Public relations helps create and maintain a relationship between the two. When an organization experiences a success or failure public relations is put into place to help rally public support. The intent of this thesis aims to determine the tone of President Obama in U.S. newspapers and social media in association with the capturing and killing of terrorist Osama bin Laden. This thesis examines a variety of U.S. newspapers by region and social media. Newspaper articles related to the topic were retrieved from six regional newspapers: Columbus Dispatch, Houston Chronicle, Charlotte Observer, New York Times, Los Angeles Times, and Washington Post. From these papers, a specific time period was chosen to examine articles. The months of April, June, and August 2012 were chosen. During these months, a keyword search for “osama bin laden” was used. Articles that contained this keyword were then examined and coded. Blogs and Facebook pages of the Republican and Democratic Party were examined. Two political blogs, Daily Kos and Red State, were chosen. From these blogs and Facebook pages, the same time period was chosen as for the newspapers. The keyword search was also the same, “osama bin laden”

The Spitzer Survey of the Small Magellanic Cloud: Discovery of Embedded Protostars in the HII Region NGC 346

We use Spitzer Space Telescope observations from the Spitzer Survey of the Small Magellanic Cloud (S3MC) to study the young stellar content of N66, the largest and brightest HII region in the SMC. In addition to large numbers of normal stars, we detect a significant population of bright, red infrared sources that we identify as likely to be young stellar objects (YSOs). We use spectral energy distribution (SED) fits to classify objects as ordinary (main sequence or red giant) stars, asymptotic giant branch stars, background galaxies, and YSOs. This represents the first large-scale attempt at blind source classification based on Spitzer SEDs in another galaxy. We firmly identify at least 61 YSOs, with another 50 probable YSOs; only one embedded protostar in the SMC was reported in the literature prior to the S3MC. We present color selection criteria that can be used to identify a relatively clean sample of YSOs with IRAC photometry. Our fitted SEDs indicate that the infrared-bright YSOs in N66 have stellar masses ranging from 2 Msun to 17 Msun, and that approximately half of the objects are Stage II protostars, with the remaining YSOs roughly evenly divided between Stage I and Stage III sources. We find evidence for primordial mass segregation in the HII region, with the most massive YSOs being preferentially closer to the center than lower-mass objects. Despite the low metallicity and dust content of the SMC, the observable properties of the YSOs appear consistent with those in the Milky Way. Although the YSOs are heavily concentrated within the optically bright central region of N66, there is ongoing star formation throughout the complex and we place a lower limit on the star formation rate of 3.2 x 10^-3 Msun/yr over the last ~1 Myr.Comment: 13 pages, 5 figures (3 in color), 2 tables. Accepted for publication in Ap

Pentraxin-3 is a PI3K signaling target that promotes stem cell–like traits in basal-like breast cancers

Basal-like breast cancers (BLBCs) exhibit hyperactivation of the phosphoinositide 3-kinase (PI3K) signaling pathway because of the frequent mutational activation of the PIK3CA catalytic subunit and the genetic loss of its negative regulators PTEN (phosphatase and tensin homolog) and INPP4B (inositol polyphosphate-4-phosphatase type II). However, PI3K inhibitors have had limited clinical efficacy in BLBC management because of compensatory amplification of PI3K downstream signaling loops. Therefore, identification of critical PI3K mediators is paramount to the development of effective BLBC therapeutics. Using transcriptomic analysis of activated PIK3CA-expressing BLBC cells, we identified the gene encoding the humoral pattern recognition molecule pentraxin-3 (PTX3) as a critical target of oncogenic PI3K signaling. We found that PTX3 abundance is stimulated, in part, through AKT- and nuclear factor κB (NF-κB)-dependent pathways and that presence of PTX3 is necessary for PI3K-induced stem cell-like traits. We further showed that PTX3 expression is greater in tumor samples from patients with BLBC and that it is prognostic of poor patient survival. Our results thus reveal PTX3 as a newly identified PI3K-regulated biomarker and a potential therapeutic target in BLBC

A coastal current in winter : 2. Wind forcing and cooling of a coastal current east of Cape Cod

Author Posting. © American Geophysical Union, 2008. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 113 (2008): C10014, doi:10.1029/2008JC004750.The combined effect of cooling and wind-driven buoyancy flux (WDBF) on a buoyant coastal current east of Cape Cod is investigated using observations and process-oriented numerical modeling. Theoretical considerations show that with the moderately strong surface density gradients observed in the Outer Cape Cod Coastal Current, WDBF can substantially exceed the buoyancy loss due to cooling, especially during intense winter storms. Evidence of deep convection associated with strong negative WDBF during downwelling-favorable winds is clearly seen in the moored observations. A simplified two-dimensional numerical model is used to illustrate the evolution of wind- and buoyancy-driven cross-shelf overturning circulation in response to surface cooling and episodic storm events. The simulation confirms that WDBF plays an important role in driving subduction of cold surface water at the offshore surface outcrop of the coastal current font. The presence of the coastal current is also shown to block onshore Ekman transport. As a result, the downwelling circulation in a cross-shore plane is predicted to have a complex multicell structure, in which exchange between the inner shelf and midshelf is restricted. The downwelling circulation has a major impact on the cross-shelf origin of cold, dense shelf waters contributing to intermediate layers of the Wilkinson Basin of the Gulf of Maine.This work was supported by the Coastal Ocean Institute of the Woods Hole Oceanographic Institution and the WHOI SeaGrant Office under grant NA06OAR4170021. G.G. was supported by the Office of Naval Research as part of the AWACS program under grant N00014-05-1-0410. A.S. was supported, in part, by WHOI Post-Doctoral Scholarship

S. pneumoniae transmission according to inclusion in conjugate vaccines: Bayesian analysis of a longitudinal follow-up in schools

BACKGROUND: Recent trends of pneumococcal colonization in the United States, following the introduction of conjugate vaccination, indicate that non-vaccine serotypes tend to replace vaccine serotypes. The eventual extent of this replacement is however unknown and depends on serotype-specific carriage and transmission characteristics. METHODS: Here, some of these characteristics were estimated for vaccine and non-vaccine serotypes from the follow-up of 4,488 schoolchildren in France in 2000. A Bayesian approach using Markov chain Monte Carlo data augmentation techniques was used for estimation. RESULTS: Vaccine and non-vaccine serotypes were found to have similar characteristics: the mean duration of carriage was 23 days (95% credible interval (CI): 21, 25 days) for vaccine serotypes and 22 days (95% CI: 20, 24 days) for non-vaccine serotypes; within a school of size 100, the Secondary Attack Rate was 1.1% (95% CI: 1.0%, 1.2%) for both vaccine and non-vaccine serotypes. CONCLUSION: This study supports that, in 3–6 years old children, no competitive advantage exists for vaccine serotypes compared to non-vaccine serotypes. This is an argument in favour of important serotype replacement. It would be important to validate the result for infants, who are known to be the main reservoir in maintaining transmission. Overall reduction in pathogenicity should also be taken into account in forecasting the future burden of pneumococcal colonization in vaccinated populations

Lexical access speed and the development of phonological recoding during immediate serial recall

A recent Registered Replication Report (RRR) of the development of verbal rehearsal during serial recall revealed that children verbalized at younger ages than previously thought, but did not identify sources of individual differences. Here, we use mediation analysis to reanalyze data from the 934 children ranging from 5 to 10 years old from the RRR for that purpose. From ages 5 to 7, the time taken for a child to label pictures (i.e. isolated naming speed) predicted the child’s spontaneous use of labels during a visually presented serial reconstruction task, despite no need for spoken responses. For 6- and 7-year-olds, isolated naming speed also predicted recall. The degree to which verbalization mediated the relation between isolated naming speed and recall changed across development. All relations dissipated by age 10. The same general pattern was observed in an exploratory analysis of delayed recall for which greater demands are placed on rehearsal for item maintenance. Overall, our findings suggest that spontaneous phonological recoding during a standard short-term memory task emerges around age 5, increases in efficiency during the early elementary school years, and is sufficiently automatic by age 10 to support immediate serial recall in most children. Moreover, the findings highlight the need to distinguish between phonological recoding and rehearsal in developmental studies of short-term memory

Horizontal Branch Stars: The Interplay between Observations and Theory, and Insights into the Formation of the Galaxy

We review HB stars in a broad astrophysical context, including both variable and non-variable stars. A reassessment of the Oosterhoff dichotomy is presented, which provides unprecedented detail regarding its origin and systematics. We show that the Oosterhoff dichotomy and the distribution of globular clusters (GCs) in the HB morphology-metallicity plane both exclude, with high statistical significance, the possibility that the Galactic halo may have formed from the accretion of dwarf galaxies resembling present-day Milky Way satellites such as Fornax, Sagittarius, and the LMC. A rediscussion of the second-parameter problem is presented. A technique is proposed to estimate the HB types of extragalactic GCs on the basis of integrated far-UV photometry. The relationship between the absolute V magnitude of the HB at the RR Lyrae level and metallicity, as obtained on the basis of trigonometric parallax measurements for the star RR Lyrae, is also revisited, giving a distance modulus to the LMC of (m-M)_0 = 18.44+/-0.11. RR Lyrae period change rates are studied. Finally, the conductive opacities used in evolutionary calculations of low-mass stars are investigated. [ABRIDGED]Comment: 56 pages, 22 figures. Invited review, to appear in Astrophysics and Space Scienc

TRIM5α Modulates Immunodeficiency Virus Control in Rhesus Monkeys

The cytoplasmic TRIM5α proteins of certain mammalian lineages efficiently recognize the incoming capsids of particular retroviruses and potently restrict infection in a species-specific manner. Successful retroviruses have evolved capsids that are less efficiently recognized by the TRIM5α proteins of the natural hosts. To address whether TRIM5α contributes to the outcome of retroviral infection in a susceptible host species, we investigated the impact of TRIM5 polymorphisms in rhesus monkeys on the course of a simian immunodeficiency virus (SIV) infection. Full-length TRIM5α cDNAs were derived from each of 79 outbred monkeys and sequenced. Associations were explored between the expression of particular TRIM5 alleles and both the permissiveness of cells to SIV infection in vitro and clinical sequelae of SIV infection in vivo. Natural variation in the TRIM5α B30.2(SPRY) domain influenced the efficiency of SIVmac capsid binding and the in vitro susceptibility of cells from the monkeys to SIVmac infection. We also show the importance in vivo of the interaction of SIVmac with different allelic forms of TRIM5, demonstrating that particular alleles are associated with as much as 1.3 median log difference in set-point viral loads in SIVmac-infected rhesus monkeys. Moreover, these allelic forms of TRIM5 were associated with the extent of loss of central memory (CM) CD4+ T cells and the rate of progression to AIDS in the infected monkeys. These findings demonstrate a central role for TRIM5α in limiting the replication of an immunodeficiency virus infection in a primate host

Serotype-specific mortality from invasive Streptococcus pneumoniae disease revisited

BACKGROUND: Invasive infection with Streptococcus pneumoniae (pneumococci) causes significant morbidity and mortality. Case series and experimental data have shown that the capsular serotype is involved in the pathogenesis and a determinant of disease outcome. METHODS: Retrospective review of 464 cases of invasive disease among adults diagnosed between 1990 and 2001. Multivariate Cox proportional hazard analysis. RESULTS: After adjustment for other markers of disease severity, we found that infection with serotype 3 was associated with an increased relative risk (RR) of death of 2.54 (95% confidence interval (CI): 1.22–5.27), whereas infection with serotype 1 was associated with a decreased risk of death (RR 0.23 (95% CI, 0.06–0.97)). Additionally, older age, relative leucopenia and relative hypothermia were independent predictors of mortality. CONCLUSION: Our study shows that capsular serotypes independently influenced the outcome from invasive pneumococcal disease. The limitations of the current polysaccharide pneumococcal vaccine warrant the development of alternative vaccines. We suggest that the virulence of pneumococcal serotypes should be considered in the design of novel vaccines