3 research outputs found

    Confirmation de QTL et validation de marqueurs SNPs associés à la résistance du niébé à Colletotrichum capsici, agent responsable de la maladie des taches brunes

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    Le niĂ©bĂ© (Vigna unguiculata (L.) Walp.) est une lĂ©gumineuse Ă  graine très importante et constitue la principale source de protĂ©ines vĂ©gĂ©tales pour l’alimentation des populations d’Afrique Subsaharienne. Sa production au Burkina Faso est entravĂ©e par la maladie des taches brunes provoquĂ©e par un champignon, Colletotrichum capsici (Syd.) Butler et Bisby. C’est dans la perspective d’accroĂ®tre la productivitĂ© du niĂ©bĂ© que nous avons entrepris de renforcer la lutte variĂ©tale contre cet agent pathogène. L’identification de marqueurs SNPs (Single Nucleotide Polymorphism) et QTL liĂ©s Ă  la rĂ©sistance Ă  la maladie des taches brunes a Ă©tĂ© entrepris Ă  partir d’une population biparentale F2 issus du croisement entre la variĂ©tĂ© sensible TiligrĂ© et celle rĂ©sistante KN-1. L’analyse QTL de la rĂ©sistance du niĂ©bĂ© Ă  C. capsici Ă  partir de la mĂ©thode ICIM add. a permis de confirmer et de valider respectivement un QTL majeur dĂ©nommĂ© qBBDR2.1 et 9 marqueurs SNPs convertis, lesquels ont Ă©tĂ© cartographiĂ©s sur le chromosome Vu02 du niĂ©bĂ©. Ce QTL dominant a prĂ©sentĂ© des effets additifs Ă©levĂ©s liĂ©s aux allèles favorables de KN-1 et des valeurs de PVE de l’ordre de 51,50% et 55,33%, respectivement aux 21ème et 28ème JAI. English title: Confirmation of QTL mapping and validation of SNPs markers associated to cowpea resistance to Colletotrichum capsici, causal agent of brown blotch disease Cowpea (Vigna unguiculata (L.)Walp.) is one of the most important grain legume crops and constitutes the main source of plant protein for people food in sub-Saharan Africa. Cowpea production in Burkina Faso is constrained by brown blotch disease caused by a fungal,  Colletotrichum capsici (Syd.) Butler and Bisby. In order to increase cowpea productivity we initiated a project to enhance host plant resistance to control the pathogen. The identification of SNP (Single Nucleotide Polymorphism) markers and QTL associated with brown blotch disease resistance was undertaken from a bi-parental F2 population resulting from a cross between the sensitive variety Tiligre and the resistant KN-1 to the disease. QTL analysis of cowpea resistance to C. capsici using the ICIM add method. Allowed to confirm and validate respectively a major QTL named qBBDR2.1 and 9 converted SNP markers, which were mapped on cowpea chromosome Vu02. This dominant QTL showed higher additive effects associated to alleles from KN-1 and PVE values of 51.50% and 55.33% respectively at 21 and 28 days after inoculatio
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