Buněčná lokalizace rezistentních proteinů Vga(A)LC a Msr(A) prostřednictvím fluorescenční mikroskopie

Vga(A)LC a Msr(A) jsou klinicky významnými rezistentními proteiny u stafylokoků, které udělují rezistenci k inhibitorům translace. Patří mezi ARE ABC-F podrodinu proteinů, která je součástí ABC transportérů. Narozdíl od typických ABC transportérů, ABC-F proteiny nemají transmembránové domény, které jsou zodpovědné za transport látek přes membránu. Proto pro ně není charakteristická transportní funkce, ale regulační nebo rezistenční funkce. Jejich mechanismus působení na ribozomu byl popsán teprve nedávno, kde tyto proteiny vytěsňují antibiotikum z ribozomu. Stále jsou však některé aspekty jejich funkce neobjasněné. Například to, jaký je význam umístění Vga(A) na membráně, které bylo detekováno v membránové frakci nikoliv v ribozomální. V této práci jsem prostřednictvím fluorescenční mikroskopie pozorovala subcelulární lokalizaci rezistenčních fúzních proteinů Vga(A)LC-mEos2, Vga(A)LC- GFP a Msr(A)- eqFP650 v živých buňkách S. aureus, za různých kultivačních podmínek. Ukázalo se, že Vga(A)LC- GFP i Msr(A)- eqFP650 se vyskytují v ohnisku blízko membrány. V závislosti na ATPázové aktivitě nebo přítomnosti antibiotika se lokalizace Msr(A)- eqFP650 v buňce mění z ohniskové na difuzní, pravděpodobně na ribozomech, což navrhuje hypotézu o duálním mechanismu ARE ABC-F proteinů. Druhým cílem práce bylo...Vga(A)LC and Msr(A) are clinically significant resistant proteins in staphylococci that confer resistance to translational inhibitors. They belong to ARE ABC-F protein subfamily, which is part of ABC transporters. Unlike typical ABC transporters, ABC-F proteins do not have transmembrane domains that are responsible for the transport of substances through the membrane. Therefore, they do not have characteristic transport function but regulatory or resistance function. Their mechanism of action on the ribosome has been described only recently, where these proteins displace the antibiotic from the ribosome. However, some aspects of their function are still unclear. For example, what is the function of the Vga(A) location on a membrane that has been detected in the membrane fraction but not in the ribosomal. In this work, using fluorescence microscopy, I observed subcellular localization of the Vga(A)LC-mEos2, Vga(A)LC-GFP and Msr(A)-eqFP650 resistant fusion proteins in live cells of S. aureus under different culture conditions . It has been shown that Vga(A)LC-GFP and Msr(A)-eqFP650 occur in a foci near the membrane. Depending on ATPase activity or the presence of an antibiotic, the localization of Msr(A)-eqFP650 in the cell changes from focal to diffuse, presumably on ribosomes, suggesting a...Katedra genetiky a mikrobiologieDepartment of Genetics and MicrobiologyFaculty of SciencePřírodovědecká fakult

Double moral hazard in state-owned enterprises (SOES) in Vietnam case of Vietnam ship-building industry group (VINASHIN)

Thesis(Master) --KDI School:Master of Public Policy,2012OutstandingmasterpublishedNguyen, Thi Bich Ngoc

Blended Learning for Secondary Schools in Nam Dinh Province to Satisfy New Standards: The Current Situation and Proposed Models

We offered blended learning models for high schools in Nam Dinh province to satisfy Vietnam's new criteria. These models were based on general approaches to issues, theoretical research, and field research based on surveys and anket questionnaires conducted throughout the area. The results of a survey demonstrate that high school teachers in Nam Dinh have gained a fundamental grasp of blended learning and have, in practice, embraced both online and face-to-face instruction, particularly during the height of the Covid-19 outbreak. However, there was not a standard model for blended learning, therefore it was only used by a few persons. In other contexts, the concept of "blended learning" referred to what was effectively a face-to-face session that was broadcast over the Internet without the necessary adjustments being made to the content, methodology, or evaluation. As a result, we offer a number of different ways to blended learning for high schools in Nam Dinh in order to improve the quality of education provided throughout the province

The Effects of Collaborative Learning on Young ESL Learners’ L2 Anxiety and Speaking Performance

Foreign Language Anxiety (FLA) is one of the issues of interest attracting researchers in recent decades. However, while collaborative learning introduced a prospective tool for FLA, it has not been much researched in the L2 classroom context, particularly in Vietnam. This paper focuses on using collaborative learning to reduce foreign language anxiety and enhance the L2 speaking performance of young learners at an English center in Ho Chi Minh City. A combination of tools, including Aydin et al.’s (2017) Children Foreign Language Anxiety Scale (CFLAS) for the pre-tests and post-tests, the teacher’s diary, and follow-up interviews, was used to measure the changes in learner’ FLA level and speaking performance. After five-week implementations, these learners’ FLA was slightly alleviated, and their speaking performance was improved using a collaborative learning approach. Moreover, learners were found to have positive attitudes and experience with learning in the new approach. These findings implied that collaborative learning could be a potential treatment to help L2 learners uncover their anxious selves and find more confidence in using the target languag

The Combined Use of Pediococcus pentosaceus and Fructooligosaccharide Improves Growth Performance, Immune Response, and Resistance of Whiteleg Shrimp Litopenaeus vannamei Against Vibrio parahaemolyticus

In this study, we evaluated the effect of probiotic bacteria Pediococcus pentosaceus supplemented at different inclusion levels in a control diet [basal diet containing 0.5% fructooligosaccharide (FOS)] on the growth performance, feed conversion ratio, immune response, and the disease resistance of whiteleg shrimp Litopenaeus vannamei juveniles against Vibrio parahaemolyticus. A control diet with 0.5% FOS but without P. pentosaceus supplementation (Control) was prepared. In addition, three other test diets were also formulated: control diet supplemented with P. pentosaceus at (i) 1 x 10(6) cfu g(-1) diet (P1), (ii) 1 x 10(7) cfu g(-1) diet (P2), or (iii) 1 x 10(8) cfu g(-1) diet (P3). After a 60-day feeding trial, the experimental shrimps were challenged with V. parahaemolyticus. The results showed that dietary supplementation of P. pentosaceus significantly improved the growth performance and immune responses of L. vannamei juveniles. The juveniles that were fed with a P2 or P3 diet recorded the maximum increase in the final body weight, final length, weight gain, and survival rate. The total hemocyte counts, phenoloxidase, and lysozyme activity of shrimp fed with either of these two diets were significantly enhanced. The results also showed that juveniles fed with a P2 or P3 diet exhibited significantly lower mortality when challenged with V. parahaemolyticus. Overall results suggested that a combination of P. pentosaceus at the inclusion level of 1 x 10(7) cfu g(-1) diet (P2) and 0.5% FOS could be considered as a potential synbiotic formulation for improving the growth, health, and robustness of L. vannamei

Musculoskeletal Pain and Work-related Risk Factors among Waste Collectors in Hanoi, Vietnam: A Cross-sectional Study

BACKGROUND: Musculoskeletal disorders (MSDs) are prevalent among waste collectors (WCs) in developing countries. AIM: This study aimed to investigate the prevalence of MSDs and the factors associated with the risk of persistent musculoskeletal pain among WCs in Hanoi, Vietnam. METHODS: A cross-sectional survey was utilized to study 468 WCs in 2017. The Örebro Musculoskeletal Pain Questionnaire and a questionnaire on demographic and work conditions were used to collect data. Descriptive and multivariate logistics regression analyzes were applied at a significance level of 0.05 to examine the factors related to the risk of persistent pain. FINDINGS: About 74.4% of the participants of this study experienced MSDs in at least one body region and 9.4% reported MSDs in all 10 body sites. The lower back was reported to be the most affected followed by the neck and shoulders. The risk of persistent musculoskeletal pain was significantly associated with age (odds ratio (OR) = 2.31, confidence interval (CI) = 1.05–5.09), gender (OR = 3.29, CI = 1.28–8.44), work hours (OR = 2.35, CI = 1.12–4.92), work shift (OR = 0.48, CI = 0.26–0.92), duration of poor postures of the neck (OR = 0.31, CI = 0.13–0.76), bent back (OR = 0.4 CI = 0.18–0.92) and for medial rotation (OR = 3.01, CI = 1.42–6.36), carrying heavy objects (OR = 2.94, CI = 1.15–7.48), and experience of work dissatisfaction (OR = 3.31, CI = 1.46-7.52), stress (OR = 7.14, CI = 3.14–16.24), or anxiety (OR = 6.37, CI = 3.07–13.21). CONCLUSIONS: High prevalence of MSDs among WCs and its association with self-assessed unfavorable work postures and work-related stress implies the need of mechanical and social support at work for WC to prevent the development of MSDs and persistent pain