Production of γ-decalactone by Yarrowia lipolytica : insights into experimental conditions and operating mode optimization

BACKGROUND γ-Decalactone production from ricinoleic acid biotransformation derived from the triglycerides in castor oil by Yarrowia lipolytica, has been widely described in literature in studies concerning lipidic metabolism that leads to lactones production, interactions of cells with the lipid substrate, toxicity of produced metabolites, selection of over-producing mutants and selection of environmental conditions. RESULTS In order to improve technological aspects of γ-decalactone production, oxygen transfer rate (OTR), cell density and oil concentration effects were investigated, in batch and step-wise fed-batch cultures of Yarrowia lipolytica W29. The best γ-decalactone concentration of 5.4 ± 0.5 g L-1 was obtained for batch cultures with 60 g L-1 of cells and substrate concentration. CONCLUSION The direct influence of aeration and agitation rates, thus of OTR, on production of γ-decalactone has been demonstrated. γ-Decalactone productivity of 215 ± 19 mg L-1 h-1 was obtained with 60 g L-1 of cells and castor oil concentration in batch and step-wise fed-batch cultures of Yarrowia lipolytica. The results obtained suggest that these two strategies are good alternatives for industrial production processes.The authors thank the Project 'BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028' co-funded by the Programa Operacional Regional do Norte (ON. 2 - O Novo Norte), QREN, FEDER and Fundacao para a Ciencia e a Tecnologia (FCT) (SFRH/BD/63701/2009 PhD grant to Adelaide Braga) for the financial support provided and FCT Strategic Project PEst-OE/EQB/LA0023/2013

Microorganisms as sources of oils

A number of microorganism belonging to the genera of yeast, fungi, bacteria and microalgae have ability to accumulate substantial amounts of oil, sometimes up to an even in excess of 70% of their biomass weight under specific cultivation conditions. For nearly 100 years, the commercial opportunities of using microorganisms as sources of oils have been continuously examined. Although it was evident that microbial oils could never compete commercially with the major commodity plant oils, there were commercially opportunities for the production of some of the higher valued oils. Today, with the great progress of metabolic and genetic engineering, the developments are focus on the high value oils containing important polyunsaturated or specific fatty acids. Such oils have the potential to be used in different applications area as food, feed and oleochemistry. This review is covering the related researches about different oleaginous microorganisms for lipids production and microbial oils biosynthesis process. In add, the lipid metabolism, metabolic engineering strategies to increase lipid production and the economics of microbial oils production are introduced

Microorganisms as sources of oils

A number of microorganism belonging to the genera of yeast, fungi, bacteria and microalgae have ability to accumulate substantial amounts of oil, sometimes up to an even in excess of 70% of their biomass weight under specific cultivation conditions. For nearly 100 years, the commercial opportunities of using microorganisms as sources of oils have been continuously examined. Although it was evident that microbial oils could never compete commercially with the major commodity plant oils, there were commercially opportunities for the production of some of the higher valued oils. Today, with the great progress of metabolic and genetic engineering, the developments are focus on the high value oils containing important polyunsaturated or specific fatty acids. Such oils have the potential to be used in different applications area as food, feed and oleochemistry. This review is covering the related researches about different oleaginous microorganisms for lipids production and microbial oils biosynthesis process. In add, the lipid metabolism, metabolic engineering strategies to increase lipid production and the economics of microbial oils production are introduced

Ingenierie metabolique de la levure yarrowia lipolytica pour la production d'acides dicarboxyliques à partir d'huiles végétales

PARIS-AgroParisTech Centre Paris (751052302) / SudocSudocFranceF