Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.Publisher PDFPeer reviewe

The PTEN Phosphatase Controls Intestinal Epithelial Cell Polarity and Barrier Function: Role in Colorectal Cancer Progression

The PTEN phosphatase acts on phosphatidylinositol 3,4,5-triphosphates resulting from phosphatidylinositol 3-kinase (PI3K) activation. PTEN expression has been shown to be decreased in colorectal cancer. Little is known however as to the specific cellular role of PTEN in human intestinal epithelial cells. The aim of this study was to investigate the role of PTEN in human colorectal cancer cells.Caco-2/15, HCT116 and CT26 cells were infected with recombinant lentiviruses expressing a shRNA specifically designed to knock-down PTEN. The impact of PTEN downregulation was analyzed on cell polarization and differentiation, intercellular junction integrity (expression of cell-cell adhesion proteins, barrier function), migration (wound assay), invasion (matrigel-coated transwells) and on tumor and metastasis formation in mice. Electron microscopy analysis showed that lentiviral infection of PTEN shRNA significantly inhibited Caco-2/15 cell polarization, functional differentiation and brush border development. A strong reduction in claudin 1, 3, 4 and 8 was also observed as well as a decrease in transepithelial resistance. Loss of PTEN expression increased the spreading, migration and invasion capacities of colorectal cancer cells in vitro. PTEN downregulation also increased tumor size following subcutaneous injection of colorectal cancer cells in nude mice. Finally, loss of PTEN expression in HCT116 and CT26, but not in Caco-2/15, led to an increase in their metastatic potential following tail-vein injections in mice.Altogether, these results indicate that PTEN controls cellular polarity, establishment of cell-cell junctions, paracellular permeability, migration and tumorigenic/metastatic potential of human colorectal cancer cells

Augmented reality (AR) technology enabled learning

To create Augmented Reality enabled learning through projects for National Library.Bachelor of Engineering (Mechanical Engineering

Multi-agent base Information Retrieval System for Job Seeker and Employer

Today, everybody has to work and so, they search the associated job balancing with job agency their qualifications. For every graduated people have many opportunities in every workplace. But, they may not be free to search one job after other themselves. So, we need job agency which helps employees find work. In this paper, we propose a job agency system. The goal of this paper is to know the job which concerned with employees’ qualifications. This system includes two parts including Job seeker and employer using by multiple agents such as register agent, user interface agent, job search agent, CV creator agent and employer agent. Job seeker can use our system using interface agent and make register using register agent. Job seeker can search jobs using job search agent. If they want to apply; they create CV first and can apply the job. Employer can use the register agent search appropriate qualified employee using our system

LEARNING THE EFFICIENT ESTIMATION TECHNIQUES FOR SUCCESSFUL SOFTWARE PROJECT MANAGEMENT

The process of software project management involves planning and supervising the development of software projects to deliver a quality product within the customer’s budget and schedule. This process begins with project planning, which includes estimating the work to be done, required resources, and project schedule. Once these activities are accomplished, a project schedule is established that defines software engineering tasks, identifies responsible parties, and specifies inter-task dependencies. The paper aims to explore project management activities and techniques for estimating project size. Overall, software project management involves managing, allocating, and timing resources to develop software that meets requirements and is delivered within budget and schedule. This paper highlights the significance of employing efficient estimation methods to achieve successful software project management. Estimation plays a critical role in the software development process, as it helps project managers to determine the resources and time required for completing the project. The paper starts by introducing the concept of software project management and highlighting its importance in delivering successful projects. The advantages and disadvantages of each technique are discussed, and guidelines for selecting the appropriate technique for a specific project are provided. The paper also explores the importance of accurate estimation in agile software development and the use of estimation tools to simplify the process. Finally, the paper concludes by summarizing the key takeaways from the discussion and emphasizing the significance of efficient estimation techniques in ensuring successful software project management

Analysis on Gamma Spectra by NaI (Tl) Detector

The (3'' x 3'') NaI (Tl) detector at the Experimental Nuclear Laboratory, Physics Department, Mandalay University was used for gamma spectrum analysis incorporation with Gamma Vision 32 software. The standard gamma source 152Eu (Spectrum Technique, USA) was used to calibrate the NaI (Tl) detector. Calibration range was 122 keV to 1408 keV and showed a linear relation of 0.943 keV per channel. Sand sample from Moemauk Hot Spring, Banmaw Township, Kachin State was analyzed by gamma spectroscopy. The gamma emissions were observed at 212Pb (239 keV), 228Ac (338 keV), 208Tl (583 keV), 228Ac (911 keV) and 40K (1460 keV). With high resolution detector, such as HpGe, more gamma emissions were expected to be observed

Traditional knowledge of wild edible plants with special emphasis on medicinal uses in Southern Shan State, Myanmar

Abstract Background Myanmar is one of the hotspots of biodiversity and is a rapidly developing country. Performing floristic research in Myanmar is an urgent issue, and ethnobotanical studies of wild edible plants (WEPs) will provide new information on natural plant resources. Method Ethnobotanical data were collected in three villages with different historical backgrounds in Southern Shan State, Myanmar. A total of 19 key informants were interviewed, and specimens were collected in the fields with the participation of key informants in June–July 2015. Group discussions were organized during 2016 and 2017 to reinforce the information on use of WEPs. DNA barcoding was used to facilitate species identification. Results A total of 83 species from 44 families of angiosperms were recorded as WEPs. Most of the species were used as wild vegetables (47 species), followed by fruits and nuts (31 species). Eighteen WEPs were consumed as medicinal foods. Differences in use of WEPs between the communities of the villages were observed. The age class of 30–39 years was more familiar with the environments where they could collect WEPs and had more knowledge of WEPs than did the older groups. The use of Elaeocarpus floribundus as an edible oil is a very interesting tradition. Conclusion WEPs play an important role in the livelihood of local communities. The indigenous society has maintained traditional knowledge of the WEPs. Historical background, land use system and surrounding vegetation could have effects on the variation in the traditional uses of WEPs. Increasing awareness of the importance of WEPs will encourage the conservation of traditional knowledge of indigenous populations

Gravitational Collapse and Black Holes on the Brane

Gravitational collapse is assumed to be one of the main problems in general relativity and astrophysics. Using simple the Taylor expansion into the bulk and black string concept one can acquire acceptable “tidal charge” black hole and continue to explore “total tidal charge”, physical mass and energy for the modified Friedmann model. Some numerical works of the interesting equations are implemented

N-Body Simulations of Homogeneous Galaxies

N-body simulations of homogeneous galaxies have been made to explore the physical proposal of critical density and mass, angular momentum and so on. Fundamental properties of galactic dynamics and structure formation have also been studied. Mathematica codes and finite element method have been briefly explored. As the situation dictates, Mathematica software is used for some detailed computations and visualization of the results

Thylacopteris minuta (Polypodiaceae), a new fern species from Myanmar

The genus Thylacopteris is a small, phylogenetically isolated genus belonging to the fern family Polypodiaceae. This study describes a new species, Thylacopteris minuta, based on collections obtained during field surveys of Shan State, Myanmar. This new species is distinct from other species of Thylacopteris in its small size and presence of sclerenchyma strands in the rhizome. This species is also distinct from the only other species of Thylacopteris with molecular data available, T. papillosa, in a plastid rbcL phylogeny of Polypodiaceae. This new discovery of Thylacopteris from Myanmar suggests that this genus is still overlooked in Southeast Asia