Spatial and temporal homogeneity of T-cell receptor gamma chain rearrangements in mycosis fungoides: A next-generation sequencing analysis

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LEC1 (NF-YB9) directly interacts with LEC2 to control gene expression in seed

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CU6 progress report 8: GAIA Report to ESA (GAIA-C6-PR-OPM-CDL-009-01)

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CU6 progress report 8

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CU6 progress report 8: GAIA Report to ESA (GAIA-C6-PR-OPM-CDL-009-01)

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CU6 spectroscopic processing SDP

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CU6 spectroscopic processing SDP: GAIA Report to ESA (GAIA-C6-PL-OPM-DK-003-03)

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CU6 spectroscopic processing SDP: GAIA Report to ESA (GAIA-C6-PL-OPM-DK-003-03)

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Besoins éducatifs en santé en Appartement de Coordination Thérapeutique

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Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed.

International audienceIn Arabidopsis (Arabidopsis thaliana), transcriptional control of seed maturation involves three related regulators with a B3domain, namely LEAFY COTYLEDON2 (LEC2), ABSCISIC ACID INSENSITIVE3 (ABI3), and FUSCA3 (ABI3/FUS3/LEC2[AFLs]). Although genetic analyses have demonstrated partially overlapping functions of these regulators, the underlyingmolecular mechanisms remained elusive. The results presented here confirmed that the three proteins bind RY DNA elements(with a 59-CATG-39 core sequence) but with different specificities for flanking nucleotides. In planta as in the moss Physcomitrellapatens protoplasts, the presence of RY-like (RYL) elements is necessary but not sufficient for the regulation of the OLEOSIN1 (OLE1)promoter by the B3AFLs. G box-like domains, located in the vicinity of the RYL elements, also are required for proper activation ofthe promoter, suggesting that several proteins are involved. Consistentwith this idea, LEC2 andABI3 showed synergistic effects onthe activation of the OLE1 promoter. What is more, LEC1 (a homolog of the NF-YB subunit of the CCAAT-binding complex)further enhanced the activation of this target promoter in the presence of LEC2 and ABI3. Finally, recombinant LEC1 and LEC2proteins produced in Arabidopsis protoplasts could form a ternary complex with NF-YC2 in vitro, providing a molecular explanationfor their functional interactions. Taken together, these results allow us to propose a molecularmodel for the transcriptional regulationof seed genes by the L-AFL proteins, based on the formation of regulatory multiprotein complexes between NF-YBs, which carry aspecific aspartate-55 residue, and B3 transcription factors