Influence of sulphide Cu (I) promoting additives concentration on acid and catalytic properties of high-silica zeolites in straight-run gasoline conversion

In present article the influence of Cu[2]S promoting additives concentration on acid and catalytic properties of high silica MFI-type zeolites is investigated in the process of conversion of straight-run gasoline fractions of gas condensate into high octane components of motor fuels. It was shown that zeolite modified with 1% of Cu[2]S nanoscaled powder possesses the highest acid centers concentration and highest catalytic activity

Salvador Dali and jewellery design

The history of design is an important aspect for considering the heritage of individual members of this activity. The term “art” is partly formulated from the assumption, that theexistence of this phenomenon is seen in the temporary space.Rethinking the aesthetic perception of the world, as an individual artist and as the creative discoveries and the era of the arts in general is made by literary translation. One of the brightest occasions in the history of design in 20 century was the appearance of the Spanish artist, painter, graphic artist, sculptor, director, writer and designer, Salvador Dali.The cultural situation of the 20th century as well as considering surrealismto be a special phenomenon in the development of Europeanculture cause the interest to historians and theorists of culture

Разработка автоматизированной системы управления печи подогрева сырой нефти установки подготовки нефти

Разработка автоматизированной системы управления печи подогрева сырой нефти установки подготовки нефти, которая позволит улучшить качественные показатели сырой нефти для дальнейшей переработки в товарную нефтьDevelopment of an automated control system for the furnace for heating crude oil of an oil preparation unit, which will improve the quality indicators of crude oil for further processing into commercial oi

Time-resolved generation of membrane potential by ba3 cytochrome c oxidase from Thermus thermophilus coupled to single electron injection into the O and OH states

peer-reviewedTwo electrogenic phases with characteristic times of ~ 14 μs and ~ 290 μs are resolved in the kinetics of membrane potential generation coupled to single-electron reduction of the oxidized “relaxed” O state of ba3 oxidase from T. thermophilus (O → E transition). The rapid phase reflects electron redistribution between CuA and heme b. The slow phase includes electron redistribution from both CuA and heme b to heme a3, and electrogenic proton transfer coupled to reduction of heme a3. The distance of proton translocation corresponds to uptake of a proton from the inner water phase into the binuclear center where heme a3 is reduced, but there is no proton pumping and no reduction of CuB. Single-electron reduction of the oxidized “unrelaxed” state (OH → EH transition) is accompanied by electrogenic reduction of the heme b/heme a3 pair by CuA in a “fast” phase (~ 22 μs) and transfer of protons in “middle” and “slow” electrogenic phases (~ 0.185 ms and ~ 0.78 ms) coupled to electron redistribution from the heme b/heme a3 pair to the CuB site. The “middle” and “slow” electrogenic phases seem to be associated with transfer of protons to the proton-loading site (PLS) of the proton pump, but when all injected electrons reach CuB the electronic charge appears to be compensated by back-leakage of the protons from the PLS into the binuclear site. Thus proton pumping occurs only to the extent of ~ 0.1 H+/e−, probably due to the formed membrane potential in the experiment.ACCEPTEDpeer-reviewe

Specific inhibition of proton pumping by the T315V mutation in the K channel of cytochrome ba3 from Thermus thermophilus

Cytochrome ba3 from Thermus thermophilus belongs to the B family of heme‑copper oxidases and pumps protons across the membrane with an as yet unknown mechanism. The K channel of the A family heme‑copper oxidases provides delivery of a substrate proton from the internal water phase to the binuclear heme‑copper centre (BNC) during the reductive phase of the catalytic cycle, while the D channel is responsible for transferring both substrate and pumped protons. By contrast, in the B family oxidases there is no D-channel and the structural equivalent of the K channel seems to be responsible for the transfer of both categories of protons. Here we have studied the effect of the T315V substitution in the K channel on the kinetics of membrane potential generation coupled to the oxidative half-reaction of the catalytic cycle of cytochrome ba3. The results suggest that the mutated enzyme does not pump protons during the reaction of the fully reduced form with molecular oxygen in a single turnover. Specific inhibition of proton pumping in the T315V mutant appears to be a consequence of inability to provide rapid (τ ~ 100 μs) reprotonation of the internal transient proton donor(s) of the K channel. In contrast to the A family, the K channel of the B-type oxidases is necessary for the electrogenic transfer of both pumped and substrate protons during the oxidative half-reaction of the catalytic cycle.Peer reviewe

Оценка гидрогеодинамического влияния режима эксплуатации скважин на основе статистических функций

Исследовано воздействие работы эксплуатационных скважин полигона захоронения промышленных отходов Сибирского химического комбината на колебание напоров в наблюдательных скважинах и выделение частотных составляющих техногенного и природного колебаний в спектре. Показана возможность использования функции взаимной корреляции и Фурье-анализа для оценки гидрогеодинамического влияния режима работы эксплуатационных скважин

Архитектура программного средства для аналитики и изуализации поведения пользователей

AbstractThe ba3-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence of octyl-β-d-thioglucoside as detergent. The crystals diffract to 3.8 Å, belong to the space group P2 or P21 and have unit cell dimensions of a = 80.7 Å; b = 116.0 Å; c = 156.9 Å and β = 104.4°. The asymmetric unit contains two ba3-type oxidase molecules

Exploring the cytochrome c folding mechanism: cytochrome c552 from thermus thermophilus folds through an on-pathway intermediate.

Understanding the role of partially folded intermediate states in the folding mechanism of a protein is a crucial yet very difficult problem. We exploited a kinetic approach to demonstrate that a transient intermediate of a thermostable member of the widely studied cytochrome c family (cytochrome c552 from Thermus thermophilus) is indeed on-pathway. This is the first clear indication of an obligatory intermediate in the folding mechanism of a cytochrome c. The fluorescence properties of this intermediate demonstrate that the relative position of the heme and of the only tryptophan residue cannot correspond to their native orientation. Based on an analysis of the three-dimensional structure of cytochrome c552, we propose an interpretation of the data which explains the residual fluorescence of the intermediate and is consistent with the established role played by some conserved interhelical interactions in the folding of other members of this family. A limited set of topologically conserved contacts may guide the folding of evolutionary distant cytochromes c through the same partially structured state, which, however, can play different kinetic roles, acting either as an intermediate or a transition state