Cyclic AMP deficiency, modifier-mutations, and instability of the cr-1 phenotype

Cyclic AMP deficiency, modifier-mutations, and instability of the cr-1 phenotyp

Derepression of tyrosinase by sexual stimulation

Derepression of tyrosinase by sexual stimulatio

Excretion of low molecular weight, folin-positive metabolites by the female receptor mycelium, in response to mating.

Excretion of low molecular weight, folin-positive metabolites by the female receptor mycelium, in response to mating

A distinctive response to concanavalin A-mediated agglutination shown by cells from two different slime strains

Response of slime strains to concanavalin

Mutations affecting accumulation of glycogen

Mutations affecting accumulation of glycoge

Role of the cell wall on the expression of osmotic-sensitive (os-1) and temperature-sensitive (cot-1) phenotypes of N. crassa. A comparative study on mycelial and wall-less phenotypes of the slime variant

Ascospore segregants ( slime -like) of the triple mutant fz(fuzzy);sg(spontaneous germination) os-1(osmotic) ( slime ; Emerson 1963. Genetica 34:162-182) of Neurospora crassa germinate as a plasmodium which, after some time, results in a morphologically abnormal mycelium. If the mycelium of a slime -like isolate is cultured under high osmotic pressure (Nelson et al. 1975. Neurospora Newsl. 22:15-16), it releases cells lacking walls which proliferate as spheroplasts

Reducing Stigma toward the Transgender Community: An Evaluation of a Humanizing and Perspective-Taking Intervention

Transgender (TG) individuals, or those whose gender identities, expressions and/or behaviors differ from their biological sex (Kirk & Kulkarni, 2006) feel there is a pervasive pattern of discrimination and prejudice directed toward them (Lombardi et al., 2001). In comparison to their heterosexual peers, LGBT youth and emerging adults are at increased risk for a host of adverse outcomes including: suicide, depression, harassment, and victimization (IOM, 2011). Stigma has been characterized as encompassing several components: labeling, making an association between the label and a negative stereotype, separating those who are different as an “out-group” and discriminating. In a recent analysis of the transgender experience, Hill (2002) described three key constructs that can be used to understand negative emotions and behaviors toward transgender individuals: transphobia – an emotional disgust toward gender non-conforming individuals; genderism – a belief that gender non-conforming individuals are pathological or disordered; gender bashing – assault or harassment of gender non-conforming individuals. Recent work on minority stress posits a distal-proximal model of stress in which a person identifies with and makes proximal, distal social attitudes that can have negative effects on their psychological well-being (Meyer, 2003). Thus, stigmatized attitudes and behaviors not only have the potential to contribute to violence or discriminatory behavior but also have a direct impact on the psychological health of the target individual. Thus, the question of how to change negative attitudes and behaviors toward TG individuals is paramount. Researchers have sought to develop interventions aimed at reducing stigma with three basic strategies identified: protest, education and contact (Corrigan & Penn, 1999). However, to date only two such strategies have garnered empirical support: contact and education. In relation to mental illness, education strategies have received limited support (Holmes et al., 1999; see Luty et al., 2007 for an exception). In contrast, contact-based interventions yield the most dramatic changes in attitudes and behaviors; contact involving media depictions have also been demonstrated to yield positive attitude change. Comparing traditional diagnosis-centered teaching about mental illness to a humanizing approach that required students to write a first-person narrative about suffering from a mental illness, Mann and Himelein (2008) found that attitudes changed only when students were required to adopt the perspective of a mentally ill individual. In their recent meta-analysis, Pettigrew and Tropp (2006) demonstrated that contact reduces prejudice and is particularly effective when it occurs under favorable conditions (e.g., conditions of equality, cooperation, and institutional support). While a wealth of research has supported the contact hypothesis related to changing negative attitudes toward ethnic minorities, the mentally ill, the homeless, gays/lesbians and other stigmatized groups, there have been a limited number of studies evaluating associations between contact and attitudes toward the TG community (Harvey, 2002; Hill & Willoughby, 2005) and no controlled studies to evaluate the efficacy of such methods. The current study extends work evaluating anti-stigma interventions to the TG community and seeks to evaluate whether attitude change will differ between participants receiving basic education about the transgender community and those who are educated about TG through media depictions of TG families and are asked to engage in a perspective-taking task. We hypothesize that participants in the humanizing condition who view a documentary and write a first-person narrative of transgender experiences will show a more significant change in transphobia, genderist attitudes and desire for social distance across time relative to participants in the education-only condition signaling less stigmatized and prejudicial attitudes at post-test. Hodson (2011) recently examined the existing contact literature and found that, consistent with Pettigrew’s (1998) focus on individual differences, intergroup contact was effective (and perhaps even more effective) among individuals who were intolerant and cognitively rigid. Religious fundamentalism has been associated with anti-homosexual sentiment (Fulton et al., 1999). Whether religiosity is similarly associated with negative attitudes toward TG individuals will be explored. Whether religiosity and prior contact with the LGBTQ community will moderate intervention outcomes will also be explored

An enzymatic defect in osmotic mutants of Neurospora crassa: lack of induction of NAD(P)ase activity during growth on casein.

An enzymatic defect in osmotic mutants of Neurospora crassa: lack of induction of NAD(P)ase activity during growth on casein

The antidiabetic drug lobeglitazone has the potential to inhibit PTP1B activity.

Protein tyrosine phosphatase 1B (PTP1B) is considered a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM), since this enzyme plays a significant role to down-regulate insulin and leptin signalling and its over expression has been implicated in the development of insulin resistance, T2DM and obesity. Some thiazolidinediones (TZD) derivatives have been reported as promising PTP1B inhibitors with anti hyperglycemic effects. Recently, lobeglitazone, a new TZD, was described as an antidiabetic drug that targets the PPAR-γ (peroxisome γ proliferator-activated receptor) pathway, but no information on its effects on PTP1B have been reported to date. We investigated the effects of lobeglitazone on PTP1B activity in vitro. Surprisingly, lobeglitazone led to moderate inhibition on PTP1B (IC50 42.8 ± 3.8 µM) activity and to a non-competitive reversible mechanism of action. As lobeglitazone inhibits PTP1B activity in vitro, we speculate that it could also target PTP1B signalling pathway in vivo and thus contribute to potentiate its antidiabetic effects

Towards a reappraisal of the phenotype of the cell wall deficient fz;sg;os-1 ( slime ) triple mutant of Neurospora crassa

Morphological mutants represent roughly 23% of seven hundred-odd distinct chromosomal loci of N. crassa, as listed by Perkins et al. (1982, Microbiol. Rev. 46:426). Probably the most radical phenotype among these strains is that of the fz;sg;os-1 ( slime ) triple mutant, which was isolated by Sterling Emerson (1963, Genetica 34:162) in a mutagenic experiment using an os-1 strain. The slime strain has been systematically referred to in the literature as a strain lacking cell wall and growing as protoplasts or plasmodium (Perkins et al. 1982). Through the years, the fragile slime structures were frequently used as a source of organelles (Martinoia et al. 1979. Arch. Microbiol. 120:31), membranes (Scarborough, 1975. J. Biol. Chem. 250:1106) or for the study of membrane-bound enzymes (Brooks et al. 1983. J. Biol. Chem. 258:13909). Slime spheroplasts practically never revert to hyphal morphology; thus, the causes for impaired cell wall synthesis were investigated and attributed either to the lack of glucan synthase activity (Leal-Morales and Ruiz-Herrera, 1985. Exp. Mycol 9:28) or to improper ultrastructural characteristics of the organelles responsible for chitin synthesis: the chitosomes (Martinez et al. 1989. Biochem. Biophys. Acta 990:45)