NuSTAR Hard X-ray View of Low-luminosity Active Galactic Nuclei: High-energy Cutoff and Truncated Thin Disk

We report the analysis of simultaneous XMM-Newton+NuSTAR observations of two low-luminosity Active Galactic Nuclei (LLAGN), NGC 3998 and NGC 4579. We do not detect any significant variability in either source over the ~3 day length of the NuSTAR observations. The broad-band 0.5-60 keV spectrum of NGC 3998 is best fit with a cutoff power-law, while the one for NGC 4579 is best fit with a combination of a hot thermal plasma model, a power-law, and a blend of Gaussians to fit an Fe complex observed between 6 and 7 keV. Our main spectral results are the following: (1) neither source shows any reflection hump with a $3\sigma$ reflection fraction upper-limits $R<0.3$ and $R<0.18$ for NGC 3998 and NGC 4579, respectively; (2) the 6-7 keV line complex in NGC 4579 could either be fit with a narrow Fe K line at 6.4 keV and a moderately broad Fe XXV line, or 3 relatively narrow lines, which includes contribution from Fe XXVI; (3) NGC 4579 flux is 60% brighter than previously detected with XMM-Newton, accompanied by a hardening in the spectrum; (4) we measure a cutoff energy $E_{\rm cut}=107_{-18}^{+27}$ keV in NGC 3998, which represents the lowest and best constrained high-energy cutoff ever measured for an LLAGN; (5) NGC 3998 spectrum is consistent with a Comptonization model with either a sphere ($\tau\approx3\pm1$) or slab ($\tau\approx1.2\pm0.6$) geometry, corresponding to plasma temperatures between 20 and 150 keV. We discuss these results in the context of hard X-ray emission from bright AGN, other LLAGN, and hot accretion flow models.Comment: 14 pages, 11 figures, 4 tables, accepted for publication in Ap

NuStar Hard X-Ray View of Low-Luminosity Active Galactic Nuclei: High-Energy Cutoff and Truncated Thin Disk

We report the analysis of simultaneous XMM-Newton+Nuclear Spectroscopic Telescope Array (NuSTAR) observations of two low-luminosity active galactic nuclei (LLAGNs), NGC3998 and NGC4579. We do not detect any significant variability in either source over the 3 day length of the NuSTAR observations. The broadband 0.560 keV spectrum of NGC3998 is best fit with a cutoff power law, while the one for NGC4579 is best fit with a combination of a hot thermal plasma model, a power law, and a blend of Gaussians to fit an Fe complex observed between 6 and 7 keV. Our main spectral results are the following: (1) neither source shows any reflection hump with 3 reflection fraction upper limits of R < 0.3 and R < 0.18 for NGC3998 and NGC4579, respectively; (2) the 67 keV line complex in NGC4579 could be fit with either a narrow Fe K line at 6.4 keV and a moderately broad Fe XXV line or with three relatively narrow lines, which include contribution from Fe XXVI; (3) the NGC4579 flux is 60% brighter than previously detected with XMM-Newton, accompanied by a hardening in the spectrum; (4) we measure a cutoff energy = - E 107+ cut 18 27 keV in NGC3998, which represents the lowest and best constrained high-energy cutoff ever measured for an LLAGN; (5) the NGC3998 spectrum is consistent with a Comptonization model with either a sphere ( 3 1) or slab ( 1.2 0.6) geometry, corresponding to plasma temperatures between 20 and 150 keV. We discuss these results in the context of hard X-ray emission from bright AGNs, other LLAGNs, and hot accretion flow models

Cellular Radiosensitivity: How much better do we understand it?

Purpose: Ionizing radiation exposure gives rise to a variety of lesions in DNA that result in genetic instability and potentially tumorigenesis or cell death. Radiation extends its effects on DNA by direct interaction or by radiolysis of H2O that generates free radicals or aqueous electrons capable of interacting with and causing indirect damage to DNA. While the various lesions arising in DNA after radiation exposure can contribute to the mutagenising effects of this agent, the potentially most damaging lesion is the DNA double strand break (DSB) that contributes to genome instability and/or cell death. Thus in many cases failure to recognise and/or repair this lesion determines the radiosensitivity status of the cell. DNA repair mechanisms including homologous recombination (HR) and non-homologous end-joining (NHEJ) have evolved to protect cells against DNA DSB. Mutations in proteins that constitute these repair pathways are characterised by radiosensitivity and genome instability. Defects in a number of these proteins also give rise to genetic disorders that feature not only genetic instability but also immunodeficiency, cancer predisposition, neurodegeneration and other pathologies. Conclusions: In the past fifty years our understanding of the cellular response to radiation damage has advanced enormously with insight being gained from a wide range of approaches extending from more basic early studies to the sophisticated approaches used today. In this review we discuss our current understanding of the impact of radiation on the cell and the organism gained from the array of past and present studies and attempt to provide an explanation for what it is that determines the response to radiation

Identification of G1-Regulated Genes in Normally Cycling Human Cells

BACKGROUND: Obtaining synchronous cell populations is essential for cell-cycle studies. Methods such as serum withdrawal or use of drugs which block cells at specific points in the cell cycle alter cellular events upon re-entry into the cell cycle. Regulatory events occurring in early G1 phase of a new cell cycle could have been overlooked. METHODOLOGY AND FINDINGS: We used a robotic mitotic shake-off apparatus to select cells in late mitosis for genome-wide gene expression studies. Two separate microarray experiments were conducted, one which involved isolation of RNA hourly for several hours from synchronous cell populations, and one experiment which examined gene activity every 15 minutes from late telophase of mitosis into G1 phase. To verify synchrony of the cell populations under study, we utilized methods including BrdU uptake, FACS, and microarray analyses of histone gene activity. We also examined stress response gene activity. Our analysis enabled identification of 200 early G1-regulated genes, many of which currently have unknown functions. We also confirmed the expression of a set of genes candidates (fos, atf3 and tceb) by qPCR to further validate the newly identified genes. CONCLUSION AND SIGNIFICANCE: Genome-scale expression analyses of the first two hours of G1 in naturally cycling cells enabled the discovery of a unique set of G1-regulated genes, many of which currently have unknown functions, in cells progressing normally through the cell division cycle. This group of genes may contain future targets for drug development and treatment of human disease

介護実習Iにおける学生のコミュニケーションの特徴 : 学内演習・実習の記録類の分析を通して

研究ノート介護実習Iの段階の学生について記録から学生のコミュニケーションに関するまとめを行った。入学当初の学生は自己表現の能力にばらつきがあり、客観的な自己イメージを十分にもっていなかった。初対面の人や会話の機会がない人との会話に苦手意識をもつ学生が多く、入学後3ヶ月を経ても話したことがない学生が7割近くいた。自己表現や自己分析をするためには、文章で表現すること、自分の正直な気持ちを表現すること、自分自身の動揺を表現する能力が必要であった。学内の演習でも初対面の学生との関わりで緊張し、動揺する中で自分自身の傾向に気付くことが可能であった。特に実習においては自分の能力に直面する場面が多いことから自身をふり返る機会が多かった