Fertility-sparing treatment for endometrial cancer and atypical endometrial hyperplasia in patients with Lynch Syndrome: Molecular diagnosis after immunohistochemistry of MMR proteins

Introduction: Lynch Syndrome (LS) represents the hereditary condition that is most frequently associated with endometrial cancer (EC). The aim of this study is to assess the presence of Lynch Syndrome (LS) in young women with mismatch repair (MMR)-deficient atypical endometrial hyperplasia (AEH) and non-myoinvasive FIGO G1 endometrioid EC and its possible impact on the outcome of conservative treatment. Methods: Six MMR-deficient cases identified from a previous cohort of 69 conservatively treated patients were selected to be screened for germline mutations in MMR genes. In each patient, the outcomes of conservative treatment for AEH and EEC, including response, relapse, progression, and pregnancy, were assessed. Results: Five out of 6 patients underwent genetic test for LS. Three out of these 5 patients showed a positive genetic test. Patient 1 showed the c.942 + 2 T>A heterozygous variant of MSH2 mutation; after 12 months of complete response, she had relapse and progression of disease. Patient 4 showed the c.2459-1G>C variant of MSH2 mutation; after complete response, she failed to achieve pregnancy; she had relapse after 24 months and underwent hysterectomy. Patient 6 showed the c.803 + 1 heterozygous variant of PMS2 mutation; she had relapse of disease after 18 months from the first complete response and then underwent hysterectomy. Conclusions: In this series, 3 out of 6 women with MMR-deficiency had LS. None of the patients achieved pregnancy, and those who responded to treatment had subsequent relapse of disease. Patients undergoing fertility-sparing treatment for atypical endometrial hyperplasia and endometrial cancer should perform MMR immunohistochemical analysis in order to screen LS

Use of Ethylenediaminetetraacetic Acid as a Scavenger for Chromium from “Wet Blue” Leather Waste: Thermodynamic and Kinetics Parameters

One serious consequence of the current consumer society is the transformation of the environment into a waste receptacle arising from human activities. Because of the potential toxic effects of chromium solid waste containing this metal there are grounds for serious concern for the tanning and leather processing industry. The application of tannery waste as organic fertilizer has led to extensive contamination by chromium in agricultural areas and may cause the accumulation of this metal in soils and plants. This work evaluated the extraction of Cr+3 and Cr+6 contained in solid waste from the leather industry through density functional theory (DFT) calculations. The Gibbs free energy calculations reveal that the chelator ethylenediaminetetraacetic acid (EDTA) forms more stable complexes with metal ions of chromium compared with the structures of the complexes [Cr(NTA)(H2O)2] and [Cr-collagen], the latter used to simulate the protein bound chrome leather

Métodos estatísticos na determinação de vazão de referência

In this study we aimed to provide the foundation studies of water availability in the Miranda riverbasin, Mato Grosso do Sul, Brazil. Therefore, this study aimed to analyze the application of Normal,Log-Normal, Weibull, Gumbel and Log-Gumbel theoretical probability methods to data of minimumflows for seven consecutive days of the basin. To verify the adherence to the estimated probabilitiesof observed frequencies, we applied the Kolmogorov-Smirnov test. The results show that the Log-Gumbel distribution show greater accuracy in the data representation of the series and reach thebest fit value of Q7,10.Neste trabalho buscou-se fornecer embasamento a estudos de disponibilidade hídricana subbacia hidrográfica do Rio Miranda, Estado de Mato Grosso do Sul, Brasil. Para tanto, objetivou-se analisar a aplicação dos métodos de distribuição de probabilidade teórica Normal, Log-Normal, Weibull, Gumbel e Log-Gumbel aos dados de vazões mínimas de sete dias consecutivos da bacia. Para verificar a aderência das probabilidades estimadas às frequências observadas, aplicou-se o teste de Kolmogorov-Smirnov. Os resultados obtidos comprovam que a distribuição Log-Gumbel demonstra maior precisão na representação dos dados da série histórica e alcança o melhor ajuste do valor da Q7,10