Evolution of the Gelasian (Pleistocene) slope turbidite systems of southern Marche (Peri-Adriatic basin, central Italy)

Examples of slope channels and canyons being diverted by structurally related sea-floor topography are commonly found both on the modern seabed and in the subsurface, in deep-water fold and thrust belts but their depositional histories have rarely been documented using examples from outcrop. The synthesis of outcrop (original geological field mapping at 1:10,000 scale) and subsurface data sets from the early Pleistocene stratigraphic succession of the Peri-Adriatic basin provides a window into the overall evolutionary pattern of large intraslope turbidite systems that, during the Gelasian (2.58–1.80 Ma), shed Apennine-derived clastic sediments into the adjacent deep-water basin. Trends from mapping and paleocurrent indicators converge to indicate that the sea-floor bathymetric expression of a thrust-related anticline, the north-trending Jesi-Nereto-Zaccheo structure, likely influenced the downslope transport direction of gravity flows and sediment dispersal pattern. During early and middle Gelasian time, coarse-grained turbidite deposition occurred on the western flank of the intraslope anticline by westerly sourced, northward-flowing turbidity currents, indicating that the opposing sea-floor topography was sufficient to cause the diversion of turbidite systems, forcing them to travel near parallel to the east-facing regional paleoslope for significant distances. By very late Gelasian time, the intraslope accommodation space on the western flank of the anticline had filled and turbidites were dispersed through dip-oriented conduits incising across the crest of the underlying structure

Dormant Mycobacterium tuberculosis fails to block phagosome maturation and shows unexpected capacity to stimulate specific human T lymphocytes

Dormancy is defined as a stable but reversible nonreplicating state of Mycobacterium tuberculosis. It is currently thought that dormant M. tuberculosis (D-Mtb) is responsible for latent tuberculosis (TB) infection. Recently, D-Mtb was also shown in sputa of patients with active TB, but the capacity of D-Mtb to stimulate specific immune responses was not investigated. We observed that purified protein derivative-specific human CD4(+) T lymphocytes recognize mycobacterial Ags more efficiently when macrophages are infected with D-Mtb instead of replicating M. tuberculosis (R-Mtb). The different Ag recognition occurs even when the two forms of mycobacteria equally infect and stimulate macrophages, which secrete the same cytokine pattern and express MHC class I and II molecules at the same levels. However, D-Mtb but not R-Mtb colocalizes with mature phagolysosome marker LAMP-1 and with vacuolar proton ATPase in macrophages. D-Mtb, unlike R-Mtb, is unable to interfere with phagosome pH and does not inhibit the proteolytic efficiency of macrophages. We show that D-Mtb downmodulates the gene Rv3875 encoding for ESAT-6, which is required by R-Mtb to block phagosome maturation together with Rv3310 gene product SapM, previously shown to be downregulated in D-Mtb. Thus, our results indicate that D-Mtb cannot escape MHC class II Ag-processing pathway because it lacks the expression of genes required to block the phagosome maturation. Data suggest that switching to dormancy not only represents a mechanism of survival in latent TB infection, but also a M. tuberculosis strategy to modulate the immune response in different stages of TB

Using a coherent hydrophone array for observing sperm whale range, classification, and shallow-water dive profiles

Sperm whales in the New England continental shelf and slope were passively localized, in both range and bearing, and classified using a single low-frequency (<2500 Hz), densely sampled, towed horizontal coherent hydrophone array system. Whale bearings were estimated using time-domain beamforming that provided high coherent array gain in sperm whale click signal-to-noise ratio. Whale ranges from the receiver array center were estimated using the moving array triangulation technique from a sequence of whale bearing measurements. Multiple concurrently vocalizing sperm whales, in the far-field of the horizontal receiver array, were distinguished and classified based on their horizontal spatial locations and the inter-pulse intervals of their vocalized click signals. The dive profile was estimated for a sperm whale in the shallow waters of the Gulf of Maine with 160 m water-column depth located close to the array's near-field where depth estimation was feasible by employing time difference of arrival of the direct and multiply reflected click signals received on the horizontal array. By accounting for transmission loss modeled using an ocean waveguide-acoustic propagation model, the sperm whale detection range was found to exceed 60 km in low to moderate sea state conditions after coherent array processing.National Science Foundation (U.S.)United States. Office of Naval Researc

Individual, unit and vocal clan level identity cues in sperm whale codas

Fieldwork was supported by Discovery and Equipment grants to H.W. from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Whale and Dolphin Conservation Society. S.G. and L.R. were supported by the Marine Alliance for Science and Technology for Scotland (MASTs) pooling initiative and their support is gratefully acknowledged. MASTs is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions. S.G. was also supported by an NSERC Postgraduate Scholarship (PGS-M), an NSERC Canadian Graduate Scholarship (CGS-D), the Izaak Killam Memorial Scholarship, the Patrick F. Lett Fund, the Dalhousie’s Presidents Award, and an FNU fellowship for the Danish Council for Independent Research from the Ministry of Higher Education and Science supplemented by a Sapere Aude Research Talent Award.The ‘social complexity hypothesis’ suggests that complex social structure is a driver of diversity in animal communication systems. Sperm whales have a hierarchically structured society in which the largest affiliative structures, the vocal clans, are marked on ocean-basin scales by culturally transmitted dialects of acoustic signals known as ‘codas’. We examined variation in coda repertoires among both individual whales and social units—the basic element of sperm whale society—using data from nine Caribbean social units across six years. Codas were assigned to individuals using photo-identification and acoustic size measurement, and we calculated similarity between repertoires using both continuous and categorical methods. We identified 21 coda types. Two of those (‘1+1+3’ and ‘5R1’) made up 65% of the codas recorded, were shared across all units and have dominated repertoires in this population for at least 30 years. Individuals appear to differ in the way they produce ‘5R1’ but not ‘1+1+3’ coda. Units use distinct 4-click coda types which contribute to making unit repertoires distinctive. Our results support the social complexity hypothesis in a marine species as different patterns of variation between coda types suggest divergent functions, perhaps representing selection for identity signals at several levels of social structure.Publisher PDFPeer reviewe

Off-axis effects on the multipulse structure of sperm whale usual clicks with implications for sound production

Author Posting. © Acoustical Society of America, 2005. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 118 (2005): 3337-3345, doi:10.1121/1.2082707.Sperm whales (Physeter macrocephalus) produce multipulsed clicks with their hypertrophied nasal complex. The currently accepted view of the sound generation process is based on the click structure measured directly in front of, or behind, the whale where regular interpulse intervals (IPIs) are found between successive pulses in the click. Most sperm whales, however, are recorded with the whale in an unknown orientation with respect to the hydrophone where the multipulse structure and the IPI do not conform to a regular pulse pattern. By combining far-field recordings of usual clicks with acoustic and orientation information measured by a tag on the clicking whale, we analyzed clicks from known aspects to the whale. We show that a geometric model based on the bent horn theory for sound production can explain the varying off-axis multipulse structure. Some of the sound energy that is reflected off the frontal sac radiates directly into the water creating an intermediate pulse p1/2 seen in off-axis recordings. The powerful p1 sonar pulse exits the front of the junk as predicted by the bent-horn model, showing that the junk of the sperm whale nasal complex is both anatomically and functionally homologous to the melon of smaller toothed whales.This work was funded by grants to from the Office of Naval Research Grant Nos. N00014-99-1-0819 and No. N00014-01-1-0705, and the Packard Foundation

Immunogenicity of viral vaccines in the italian military

Military personnel of all armed forces receive multiple vaccinations and have been doing so since long ago, but relatively few studies have investigated the possible negative or positive interference of simultaneous vaccinations. As a contribution to fill this gap, we analyzed the response to the live trivalent measles/mumps/rubella (MMR), the inactivated hepatitis A virus (HAV), the inactivated trivalent polio, and the trivalent subunits influenza vaccines in two cohorts of Italian military personnel. The first cohort was represented by 108 students from military schools and the second by 72 soldiers engaged in a nine-month mission abroad. MMR and HAV vaccines had never been administered before, whereas inactivated polio was administered to adults primed at infancy with a live trivalent oral polio vaccine. Accordingly, nearly all subjects had baseline antibodies to polio types 1 and 3, but unexpectedly, anti-measles/-mumps/-rubella antibodies were present in 82%, 82%, and 73.5% of subjects, respectively (43% for all of the antigens). Finally, anti-HAV antibodies were detectable in 14% and anti-influenza (H1/H3/B) in 18% of the study population. At mine months post-vaccination, 92% of subjects had protective antibody levels for all MMR antigens, 96% for HAV, 69% for the three influenza antigens, and 100% for polio types 1 and 3. An inverse relationship between baseline and post-vaccination antibody levels was noticed with all the vaccines. An excellent vaccine immunogenicity, a calculated long antibody persistence, and apparent lack of vaccine interference were observed

Large-scale stratigraphic architecture and sequence analysis of an early Pleistocene submarine canyon fill, Monte Ascensione succession (Peri-Adriatic basin, eastern central Italy).

The Monte Ascensione succession (c. 2.65–2.1 Ma) is a well-exposed example of an exhumed submarine canyon fill embedded within slope hemipelagic mudstones. This gorge represented a long-lasting pathway for sediment transport and deposition and during the Gelasian delivered Apennine-derived clastic sediment to the adjacent Peri-Adriatic basin. A total of six principal lithofacies types, representing both canyon-confining hemipelagic deposits and canyon-filling turbidity current and masstransport deposits, can be delineated in the studied sedimentary succession. The canyon-fill deposits display a marked cyclic character and the component lithofacies succeed one another to form at least fifteen fining-upward stratal units, which are interpreted to represent high-frequency, unconformity- bounded depositional sequences. Variability in the vertical repetition of constituent lithofacies allows the identification of three basic styles of sequence architecture that can be interpreted in terms of differing positions along a conceptual down-canyon depositional profile. An integrated chronology, based on biostratigraphic data and on palaeomagnetic polarity measurements, strongly supports a one-to-one correlation between the sequence-bounding surfaces and oxygen isotope stages G2–78, suggesting that the most feasible sequence-engendering mechanism is that of orbitally dictated glacio-eustatic changes in sea level, which regulated timing of sediment storage on the shelf and its redistribution beyond the shelf edge. One of the most significant aspects of this study is the demonstration that processes occurring within upper slope canyons can be expected to be strongly influenced by variations in sea level; that is, the erosional and depositional features evident in these deposits can be strongly controlled by allocyclic processes rather than autocyclic or random processes

Evolution and History of Filling of Early Pleistocene, Coarse-Grained Slope Canyons (Peri-Adriatic Basin, Central Italy)

The early Pleistocene stratigraphic succession of the Peri-Adriatic Basin, eastern central Italy, records the filling of an elongate, north-south oriented piggy-back basin located east of the growing Apennine fold-thrust belt. During the Gelasian (2.58-1.80 Ma), gravel- and sand-sized sediments derived from the central Apennines were abundantly supplied into the basin through a series of transverse to longitudinal slope erosional fairways. These sediment conduits are preserved in the rock record as a series of exceptionally exposed canyon-fill successions that provide a rare opportunity to evaluate, from an outcrop perspective, how this type of deepwater depositional system evolves and fills under the indirect effects of glacio-eustatic sea-level changes. The present study uses stratigraphic sections, photopanels, paleocurrent data, and careful lithological mapping to constrain the internal organization of four of these canyon fills, which we refer to as the Ascensione, Castignano, Offida, and Notaresco canyons. A detailed facies analysis suggests that a variety of subaqueous gravity flows were involved in sediment transport and deposition, including slumps, cohesive debris flows, and high- and low-density turbidity currents. Four main lithofacies that reflect major depositional elements have been identified within the canyon-fill successions and are: (i) clastsupported conglomerates (gravel-rich channel complexes); (ii) medium- to thick-bedded sandstones (channel terminus lobes); (iii) medium- to very thin-bedded sandstones (levee-overbank); (iv) pebbly mudstones and chaotic beds (mudstone-rich mass-transport deposits). The canyon fill strata are organized in lithofacies sequences that tend to follow a predictable pattern and to define vertically stacked fining-upward packages, resulting in highly cyclic successions. Each of these fining-upward packages comprises the sedimentary record of discrete phases of canyon activity, showing transition from a higher energy depositional style to a lower energy mode of sedimentation interrupted by a period of erosion and bypass of sediment to areas further downslope. These recurring fluctuations in canyon activity and sedimentary regime are thought to be related to the switching on and off of coarse clastic sediments to the deepwater basin that, in turn, likely resulted from the contemporary glacio-eustatic changes in sea level

A method permissive to fixation and permeabilization for the multiparametric analysis of apoptotic and necrotic cell phenotype by flow cytometry

Annexin-V/propidium iodide method (A-V/PI) is a common flow cytometric method for the multiparametric analysis of cells in apoptosis. However, A-V/PI does not permit fixation and/or permeabilization of cells making impossible evaluation of intracellular markers, restricting the analysis in a narrow time frame after staining and excluding the possibility to study pathogen-infected cells. We developed a method permitting fixation and permeabilization of stained cells: Fixed Apoptotic/Necrotic (FAN) cells test. FAN relies on the same principle of A-V/PI, but uses reagents that maintain their binding and fluorescence characteristics after fixation/permeabilization: a fluorochrome-labeled anti-phosphatidylserine antibody and fluorescent amine-binding dyes. FAN was tested to discriminate apoptotic and necrotic cells using different stimuli on several cell types and results were always comparable to those obtained using A-V/PI. FAN, unlike A-V/PI, permitted to correlate cell death with intracellular and surface markers expression and to perform cytometry even two weeks after sample preparation. As fixation of stained cells inactivates infective pathogens, we used FAN in an in vitro model of Mycobacterium tuberculosis (Mtb) infection of macrophages to monitor cellular infection and cell death induction. Using a red-fluorescent Mtb, fluorochrome labeled anti-TNF- and anti-MHC class II monoclonal antibodies, FAN permitted to establish that the extent of macrophage death correlates with intracellular Mtb content and that dying cells accumulate TNF- and down-modulate MHC class II molecules. Results suggest that FAN may represent an additional tool to study programmed cell death particularly useful when fixation procedures are required for a safe infected sample analysis or to comparatively analyze multiple samples. (c) 2017 International Society for Advancement of Cytometr