4,829 research outputs found
Exploring the Microbiome of Healthy and Diseased Peri-Implant Sites Using Illumina Sequencing
Aim To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analyzed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using Principal Coordinate Analysis (PCoA), t-tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined. Results PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p\u3c0.05). Porphyromonas and Treponema were more abundant in P and while Rothia and Neisseria were higher in H (p\u3c0.05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola and P. gingivalis levels were higher in P, as well as F. alocis, F fastidiosum and T. maltophilum (p\u3c0.05). Conclusion The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harboring traditional and new pathogens. The core peri-implant microbiome harbors taxa from genera often associated with periodontal inflammation
Computational fluids dynamics (CFD) in the spatial distribution of air velocity in prototype designed for animal experimentation in controlled environments
Diagnóstico Pré-natal de SÃndrome de Wolf-Hirschhorn: a propósito de um caso
Introdução:
O SÃndrome de Wolf-Hirschhorn é uma patologia originada por uma deleção da região terminal do braço curto do cromossoma 4. O tamanho da deleção pode ser variável levando a um espectro alargado de manifestações clÃnicas. Em diagnóstico pré-natal (DPN), as alterações fetais mais frequentes incluem atraso do crescimento intra-uterino, lábio leporino e/ou fenda do palato e anomalias cardÃacas. A prevalência estimada é de 1/50.000 nascimentos afetando duas vezes mais indivÃduos do sexo feminino do que do sexo masculino.
Objectivo:
Apresentação de um caso de SÃndrome de Wolf-Hirschhorn em DPN comparando-o com outros casos publicados.
Material e métodos:
Grávida com 17semanas de gestação, referenciada para estudos cromossómicos por idade materna avançada (35 anos) e rastreio bioquÃmico positivo para trissomia 18. A análise citogenética convencional dos amniócitos cultivados foi realizada de acordo com os métodos habituais usando bandas GTG. O estudo foi complementado por técnicas de citogenética molecular (FISH) utilizando-se a sonda especÃfica para a região do SÃndrome de Wolf-Hirschhorn.
Resultados:
O estudo cromossómico efetuado, revelou uma deleção na região terminal do braço curto do cromossoma 4. A análise por FISH confirmou a existência da deleção desta região, permitindo estabelecer o cariótipo 46,XX,del(4)(p15.3).ish del(4)(p16.3p16.3)(WCHR-).
Os cariótipos efetuados aos pais foram normais.
Conclusões:
Discute-se a importância deste caso pela raridade da anomalia citogenética encontrada, assim como pela dificuldade em realizar o diagnóstico por citogenética convencional, em alguns destes casos, quando não se obtêm bandas de alta resolução
Unsteady undular bores in fully nonlinear shallow-water theory
We consider unsteady undular bores for a pair of coupled equations of
Boussinesq-type which contain the familiar fully nonlinear dissipationless
shallow-water dynamics and the leading-order fully nonlinear dispersive terms.
This system contains one horizontal space dimension and time and can be
systematically derived from the full Euler equations for irrotational flows
with a free surface using a standard long-wave asymptotic expansion.
In this context the system was first derived by Su and Gardner. It coincides
with the one-dimensional flat-bottom reduction of the Green-Naghdi system and,
additionally, has recently found a number of fluid dynamics applications other
than the present context of shallow-water gravity waves. We then use the
Whitham modulation theory for a one-phase periodic travelling wave to obtain an
asymptotic analytical description of an undular bore in the Su-Gardner system
for a full range of "depth" ratios across the bore. The positions of the
leading and trailing edges of the undular bore and the amplitude of the leading
solitary wave of the bore are found as functions of this "depth ratio". The
formation of a partial undular bore with a rapidly-varying finite-amplitude
trailing wave front is predicted for ``depth ratios'' across the bore exceeding
1.43. The analytical results from the modulation theory are shown to be in
excellent agreement with full numerical solutions for the development of an
undular bore in the Su-Gardner system.Comment: Revised version accepted for publication in Phys. Fluids, 51 pages, 9
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CaracterÃsticas fÃsicas e quÃmicas de oito genótipos de Passiflora maliformis Linn. cultivados no Distrito Federal.
Coherent vibrational modes promote the ultrafast internal conversion and intersystem crossing in thiobases
Thionated nucleobases are obtained by replacing oxygen with sulphur atoms in the canonical nucleobases. They absorb light efficiently in the near-ultraviolet, populating singlet states which undergo intersystem crossing to the triplet manifold on an ultrashort time scale with a high quantum yield. Nonetheless there are still important open questions about the primary mechanisms responsible for this ultrafast transition. Here we track both the electronic and the vibrational ultrafast excited-state dynamics towards the triplet state for solvated 4-thiothymidine (4TT) and 4-thiouracil (4TU) with sub-30 fs broadband transient absorption spectroscopy in the ultraviolet. A global and target analysis allows us to simultaneously resolve the contributions of the different electronically and vibrationally excited states to the whole data set. Our experimental results, combined with state-of-the-art quantum mechanics/molecular mechanics simulations and Damped Oscillation Associated Spectra (DOAS) and target analysis, support that the relaxation to the triplet state is mediated by conical intersections promoted by vibrational coherences through the population of an intermediate singlet state. In addition, the analysis of the coherent vibrational dynamics reveals that, despite sharing the same relaxation mechanism and similar chemical structures, 4TT and 4TU exhibit rather different geometrical deformations, characterized by the conservation of planarity in 4TU and its partial rupture in 4TT
A Unified Experimental/Theoretical Description of the Ultrafast Photophysics of Single and Double Thionated Uracils
Photoinduced processes in thiouracil derivatives have lately attracted considerable attention due to their suitability for innovative biological and pharmacological applications. Here, sub-20 fs broadband transient absorption spectroscopy in the near-UV are combined with CASPT2/MM decay path calculations to unravel the excited-state decay channels of water solvated 2-thio and 2,4-dithiouracil. These molecules feature linear absorption spectra with overlapping ππ* bands, leading to parallel decay routes which we systematically track for the first time. The results reveal that different processes lead to the triplet states population, both directly from the ππ* absorbing state and via the intermediate nπ* dark state. Moreover, the 2,4-dithiouracil decay pathways is shown to be strongly correlated either to those of 2- or 4-thiouracil, depending on the sulfur atom on which the electronic transition localizes
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