Isolamento e amplificação de DNA de amostras de solo como ferramenta para avaliar a diversidade das populações de bactérias em solos agrícolas.

Aspectos físicos e químicos do solo e seu potencial de interação com os ácidos nucléicos. Extração do DNA do solo. Métodos indiretos de extração de DNA. Métodos diretos de extração de DNA. Aplicação da PCR na microbiologia ambiental. Fatores que interferem com aplicação da técnica de PCR em amostras ambientais. Parâmetros da reação que requerem otimização. Variações da técnica de PCR. Considerações finais sobre a aplicação destas técnicas na Ecologia Microbiana.bitstream/CNPAB-2010/27425/1/doc148.pd

Symbolic Computation of Variational Symmetries in Optimal Control

We use a computer algebra system to compute, in an efficient way, optimal control variational symmetries up to a gauge term. The symmetries are then used to obtain families of Noether's first integrals, possibly in the presence of nonconservative external forces. As an application, we obtain eight independent first integrals for the sub-Riemannian nilpotent problem (2,3,5,8).Comment: Presented at the 4th Junior European Meeting on "Control and Optimization", Bialystok Technical University, Bialystok, Poland, 11-14 September 2005. Accepted (24-Feb-2006) to Control & Cybernetic

Condições hídricas do Estado da Paraíba sob diferentes cenários do tempo.

Considerando-se os cenários futuros projetados por vários modelos de simulação, as regiões semiáridas deverão ser afetadas pelas mudanças do clima. Dados climáticos históricos foram usados em conjunto com projeções através do modelo HadCM3 do centro Hadley, na determinação de componentes do balanço hídrico climático, permitindo a quantificação da deficiência hídrica, para avaliação da variação nas disponibilidades mensais e anuais de água no Estado da Paraíba, considerando-se as condições médias da linha base de 1961-1990 e os cenários do IPCC para 2020, 2050 e 2080. De acordo com estas projeções, constatou-se que reduções das disponibilidades hídricas mais elevadas irão ocorrer no primeiro semestre do ano com valores médios de deficiência acima de 200 mm no mês de abril para o ano projetado de 2080, sendo as áreas do oeste do estado as mais afetadas pela escassez de água

MFS transporters required for multidrug/multixenobiotic (MD/MX) resistance in the model yeast: understanding their physiological function through post-genomic approaches

Multidrug/Multixenobiotic resistance (MDR/MXR) is a widespread phenomenon with clinical, agricultural and biotechnological implications, where MDR/MXR transporters that are presumably able to catalyze the efflux of multiple cytotoxic compounds play a key role in the acquisition of resistance. However, although these proteins have been traditionally considered drug exporters, the physiological function of MDR/MXR transporters and the exact mechanism of their involvement in resistance to cytotoxic compounds are still open to debate. In fact, the wide range of structurally and functionally unrelated substrates that these transporters are presumably able to export has puzzled researchers for years. The discussion has now shifted towards the possibility of at least some MDR/MXR transporters exerting their effect as the result of a natural physiological role in the cell, rather than through the direct export of cytotoxic compounds, and the hypothesis that MDR/MXR transporters may have evolved in nature for other purposes than conferring chemoprotection has been gaining momentum in recent years. This review focuses on the drug transporters of the Major Facilitator Superfamily (MFS; drug:H+ antiporters) in the model yeast Saccharomyces cerevisiae. New insights into the natural roles of these transporters are described and discussed here, focusing on the knowledge obtained or suggested by post-genomic research. The new information reviewed here provides clues into the unexpectedly complex roles of these transporters, including a proposed indirect regulation of the stress response machinery and control of membrane potential and/or internal pH, with a special emphasis on a genome-wide view of the regulation and evolution of MDR/MXR-MFS transporters

Caracterização morfológica e bromatológica preliminar da batata-de-vaqueiro (Mandevilla tenuifolia (Mikan) Wood), uma planta xerófitica.

Neste trabalho foram caracterizadas preliminarmente algumas variáveis bromatológicas e morfológicas de três populações de batata-de-vaqueiro, no total de 35 plantas, coletadas no município de Casa Nova, BA, de forma a subsidiar trabalhos que visem à exploração da espécie como uma hortaliça de sequeiro.Suplemento, ref 504. Edição dos Resumos do 47. Congresso Brasileiro de Olericultura; 4. Simpósio Brasileiro sobre Cucurbitáceas, Porto Seguro, ago. 2007

Correlações fenotípicas e análise de agrupamento em populações de batata-de-vaqueiro (Mandevilla tenuifolia (Mikan) Wood.

Suplemento, ref 503. Edição dos Resumos do 47. Congresso Brasileiro de Olericultura; 4. Simpósio Brasileiro sobre Cucurbitáceas, Porto Seguro, ago. 2007

Screening of Dementia in Portuguese Primary Care: Methodology, Assessment Tools, and Main Results

The objectives of this article are as follows: (1) to describe the assessment protocol used to outline people with probable dementia in Primary Health Care; (2) to show the methodological design and procedure to obtain a representative sample of patients with probable dementia; and (3) to report the main characteristics of the sample collected in the context of the study “Characteristics and needs of people with probable dementia.” The study protocol was based on the “Community Assessment of Risk and Treatment Strategies (CARTS) Program” and is composed by a set of instruments that allow the assessment of older adults with probable dementia in several areas (health, psychological, functionality, and other). Descriptive analysis was used to characterize the final sample (n = 436). The study protocol as well as the methodological procedure to obtain the referral of research participants and data collection on the condition of people with probable dementia in Primary Health Care proved to be a valuable tool to obtain a sample of patients distributed by the full range of probable dementia in a large geographical area. Results may allocate the design of care pathways for old people with cognitive disorders to prevent, delay impairment, and/or optimize quality of life of patients

Coleção núcleo de milheto da Embrapa Milho e Sorgo.

O presente trabalho teve por objetivo organizar a Coleção Núcleo de Milheto da Embrapa Milho e Sorgo.bitstream/CNPMS-2009-09/21394/1/Doc_74.pd