Induction and synchronization of estrus in dairy cows using a single injection of PGF2α and GnRH

The aim of this study was to examine the effects of treatment with a single injection of GnRH and PGF2α on estrous response, fertility and service period. A total of 120 lactating Simmental cows were divided into four groups of 30 cows each: group PGF2α 40 was treated on the 40th day post partum with a single injection of 2 mL prostaglandin analogue (Estrumate), group PGF2α 50 was treated in the same way on the 50th day, group GnRH was treated on the 40th day post partum with a single injection of 2 mL GnRH analogue (Receptal), and the fourth group (control) was not hormonally treated. Fertility of cows was not significantly different (p>0.05). The difference in the estrous response and service-period between the control group and experimental groups was statistically significant (p<0.01)

Menadžment i kontrola programa suzbijanja i eradikacije klasične kuge svinja u Srbiji

Classical swine fever (CSF) has been present in Serbia for a long period of time as an endemic disease, and it occurs with lesser or greater intensity from year to year. Since it is a highly contagious disease of domestic and wild pigs, the outbreaks of the disease causes severe consequences for animal welfare, livestock production and economic losses to the national economies in countries where it occurs. Therefore, CSF has a great epizootiological and economic importance to the swine production, primarily because of trade restrictions for live pigs and pork products. CSF eradication and control programmes are generally based on vaccination strategies in countries with endemic occurrence of CSF or non-vaccination policies in countries which successfully eradicated or are free of CSF. Whatever the general eradication strategy is in place, in the case of an outbreak of CSF the stamping-out method is used for suppression and eradication of the disease, with strict implementation of specific veterinary-sanitary measures in the infected and surveillance zones, clearly defined by legal provisions. In the period before 2006, there were certain regions in Serbia where CSF occurred almost every year, including regions where pig farming was not the primary branch of animal husbandry. Pig identification and implementation of vaccination, as well as the control of trade of pigs from infected areas to other parts of the country were not very successful. Due to the serious losses inflicted by the disease, a new Programme of CSF control was adopted in 2006, which inter alia included the obligatory identification of pigs, registration of pig holdings and free of charge vaccination of all susceptible animals in the country, as well as the implementation of other statutory measures, including obligatory evidence of veterinary activities in the national Veterinary information system. The results obtained in the period after the implementation of the Programme of CSF control (2007, 2008 and 2009) indicate a positive impact on the reduction of outbreaks of CSF, as well as on the reduction of prevalence (p lt 0.001). Also, the effectiveness of vaccination of pigs in 2007 compared to the period before and after the implementation of the Programme was the highest (97.78%), as well as the costs of its implementation.Klasična kuga svinja (KKS) je prisutna u Srbiji prisutna duži vremenski period i sa manjim ili većim intenzitetom javlja se iz godine u godinu. Kako je u pitanju infektivna bolest svinja, a polazeći od karaktera njenog širenja sama pojava bolesti nanosi velike ekonomske štete za privredu svake zemlje. Stoga KKS ima veliki epizootiološki i ekonomski značaj za svinjarsku proizvodnju, a pre svega za stvaranje nesmetane mogućnosti odvijanja prometa svinja i proizvoda od svinjskog mesa. Program kontrole KKS se može izvoditi vakcinacijom ili biti bez nje. Kod pojave bolesti se i u jednom i drugom slučaju za suzbijanje i iskorenjivanje primenjuje metod 'stamping-out', i sprovode se određene veterinarsko-sanitarne mere u zaraženom i ugroženom dvorištu koje su decidno definisane zakonskim propisima. U proteklom periodu u Srbiji su postojali određeni regioni gde se KKS javljala skoro svake godine, a naročito je bila zabrinjavajuća pojava širenja bolesti u regione gde svinjarstvo nije primarna grana stočarstva. Kontrola prometa svinja iz zaraženih područja u druge delove zemlje bila je na niskom nivou. Zbog ozbiljnih gubitaka koje je nanosila pojava bolesti, donet je novi Program kontrole KKS koji između ostalog podrazumeva obavezno obeležavanje svinja i besplatnu vakcinaciju svih prijemčivih životinja u zemlji, kao i sprovođenje drugih zakonom propisanih mera. Dobijeni rezultati u periodu posle sprovođenja Programa kontrole KKS (2008. i 2009. godine) ukazuju na pozitivan uticaj ovih mera na smanjenje pojave žarišta KKS, kao i na smanjenje prevalance (p lt 0,001). Takođe, efikasnost sprovođenja vakcinacije svinja bila je najveća (97,78%) u 2007. godini, u odnosu na period pre i posle sprovođenja Programa

APPLICATION OF HYBRID DIBR-FUCOM-LMAW-BONFERRONI-GREY-EDAS MODEL IN MULTICRITERIA DECISION-MAKING

The selection of unmanned aerial vehicles for different purposes is a frequent topic of research. This paper presents a hybrid model of an unmanned aerial vehicle (UAV) selection using the Defining Interrelationships Between Ranked criteria (DIBR), Full Consistency Method (FUCOM), Logarithm Methodology of Additive Weights (LMAW) and grey - Evaluation based on Distance from Average Solution (G-EDAS) methods. The above-mentioned model is tested and confirmed in a case study. First of all, in the paper are defined the criteria conditioning the selection, and then with the help of experts and by applying the DIBR, FUCOM and LMAW methods, the weight coefficients of the criteria are determined. The final values of the weight coefficients are obtained by aggregating the values of the criteria weights from all the three methods using the Bonferroni aggregator. Ranking and selection of the optimal UAV from twenty-three defined alternatives is carried out using the G-EDAS method. Sensitivity analysis confirmed a high degree of consistency of the solutions obtained using other MCDM methods, as well as changing the criteria weight coefficients. The proposed model has proved to be stable; its application is also possible in other areas and it is a reliable tool for decision-makers during the selection process

Evolution of humic acids during ex situ bioremediation on a pilot level: The added value of the microbial activity

Environmental pollution is a global problem, while bioremediation technology removes pollutants from the environment using microorganisms. This study was aimed at investigating how a bioremediation process affected soil humification. In soil polluted with petroleum and its derivatives that was submitted to bioremediation, besides the total petroleum hydrocarbons and the number of microorganisms, quantitative and qualitative changes of isolated humic acids were determined during the process. The bioremediation of 150 m3 of polluted soil lasted 150 days. The level of total petroleum hydrocarbons decreased by 86.6 %, while the level of humic acids increased by 26.5 %. The elemental analysis showed the reduction of C and the H/C ratio and the increase of O and the O/C ratio of isolated humic acids during the process. The ratio of absorbencies at 465 and 665 nm also increased. Based on this and the Fourier-transform infrared spectra, it was shown that the humic acids isolated at the end of bioremediation were enriched with oxygen functional groups and aromatic structures. This study provides one of the first insights into the relationship between bioremediation and humification, as well as evidence of how hydrocarbon-degrading microorganisms have a significant influence on changes to humic acid structure during bioremediation

Supplementary data for article: Keckes, S.; Gašić, U. M.; Ćirković-Veličković, T.; Milojković-Opsenica, D.; Natić, M.; Tešić, Ž. L. The Determination of Phenolic Profiles of Serbian Unifloral Honeys Using Ultra-High-Performance Liquid Chromatography/High Resolution Accurate Mass Spectrometry. Food Chemistry 2013, 138 (1), 32–40. https://doi.org/10.1016/j.foodchem.2012.10.025

Supplementary material for: [https://doi.org/10.1016/j.foodchem.2012.10.025]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1582

Efekti primene programa kontrole trihineloze na endemskom području u Srbiji

Trichinellosis is a disease that affects both humans and animals, caused by a parasite from the Trichinellidae family and Trichinella genus. Humans get infected by consuming infected and inadequately thermally treated meat from domestic or wild animals containing cocooned infective larvae of T. spiralis. Aside from health problems caused by trichinellosis as a zoonosis, there is no doubt that it represents a serious economic problem for swine meat producers. The research in this paper has been performed in Serbia, in a region which is located at the confluence of two large rivers, and it is the geographical location which makes it an endemic area for the presence of T. spiralis. Epidemiological data pertains to the period of 1995-2003, but also to the period 2003-2009, when the implementation of the program for control and eradication of trichinellosis was complete. The diagnostic testing of cadavers of slaughtered swine for the presence of T. spiralis in the period of 1995-2003 included 41.04% of the slaughtered swine, out of which 0.4281% were positive, and the amount of economic loss was 95301000 dinars or about one million EUR. The infection was confirmed in 432 patients during the research period. The preparation of the program for the control and eradication of trichinellosis has been made in accordance with applicable laws and technological standards, with a clear determination of input and output using a cost-benefit analysis. The effects of its application show a reduction in the number of swine which are positive for the presence of T. spiralis by a factor of three times (p lt 0.01), and the present net value (PNV) and the benefit/cost ratio (B/C) show economic and epidemiological justification.Trihineloza je zajedničko oboljenje ljudi i životinja koje izaziva parazit koji pripada familiji Trichinellidae i rodu Trichinella. Čovek se zarazi tako što konzumira zaraženo, nedovoljno termički obrađeno meso domaćih i divljih životinja u kojima se nalazi učaurena infektivna larva T. spiralis. Pored zdravstvenih problema koje izaziva trihineloza kao zoonoza, nesumljivo je da ona predstavlja i ozbiljan ekonomski problem za proizvođača svinjskog mesa. Ova istraživanja su izvršena u Srbiji na teritoriji jednog regiona koji se nalazi u slivu dve velike reke, i zbog takvog geografskog položaja predstavlja endemsko područje za prisustvo T. spiralis. Epidemiološki podaci se odnose na period 1995-2003. godine, kao i na period 2003- 2009. godine kada je izvršena implementacija programa kontrole i eradikacije trihineloze. Dijagnostičkim ispitivanjem trupova zaklanih svinja na prisustvo T. spiralis u periodu 1995-2003. godina obuhvaćeno je 41.04% zaklanih svinja od čega je bilo 0.4281% pozitivnih svinja, a visina ekonomskih gubitaka iznosila je 95 301 000 dinara ili oko milion EUR-a. U toku posmatranog perioda obolele su 432 osobe. Izrada programa za kontrolu i eradikaciju trihineloze izvršena je na osnovu važećih zakona i tehnoloških standarda, uz jasnu determinaciju 'inputa' i 'outputa' pomoću 'cost-benefit' analize. Efekti njegove primene pokazuju smanjenje broja pozitivnih svinja na prisustvo T. spiralis za tri puta (p lt 0,01), a neto sadašnja vrednost (PNV) i odnos koristi i troškova (B/C) pokazuju ekonomsku i epidemiološku opravdanost

Polyphenols as Possible Agents for Pancreatic Diseases

Pancreatic cancer (PC) is very aggressive and it is estimated that it kills nearly 50% of patients within the first six months. The lack of symptoms specific to this disease prevents early diagnosis and treatment. Today, gemcitabine alone or in combination with other cytostatic agents such as cisplatin (Cis), 5-fluorouracil (5-FU), irinotecan, capecitabine, or oxaliplatin (Oxa) is used in conventional therapy. Outgoing literature provides data on the use of polyphenols, biologically active compounds, in the treatment of pancreatic cancer and the prevention of acute pancreatitis. Therefore, the first part of this review gives a brief overview of the state of pancreatic disease as well as the procedures for its treatment. The second part provides a detailed overview of the research regarding the anticancer effects of both pure polyphenols and their plant extracts. The results regarding the antiproliferative, antimetastatic, as well as inhibitory effects of polyphenols against PC cell lines as well as the prevention of acute pancreatitis are presented in detail. Finally, particular emphasis is given to the polyphenolic profiles of apples, berries, cherries, sour cherries, and grapes, given the fact that these fruits are rich in polyphenols and anthocyanins. Polyphenolic profiles, the content of individual polyphenols, and their relationships are discussed. Based on this, significant data can be obtained regarding the amount of fruit that should be consumed daily to achieve a therapeutic effect

Supplementary data for article: Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783

Supplementary material for: [https://doi.org/10.3390/biom9120783]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3774

Supplementary data for article: Mosić, M.; Trifković, J.; Vovk, I.; Gašić, U.; Tešić, Ž.; Šikoparija, B.; Milojković-Opsenica, D. Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen. Biomolecules 2019, 9 (12). https://doi.org/10.3390/biom9120783

Supplementary material for: [https://doi.org/10.3390/biom9120783]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3774

Supplementary data for article: Nešović, M.; Gašić, U.; Tosti, T.; Trifković, J.; Baošić, R.; Blagojević, S.; Ignjatović, L.; Tešić, Ž. Physicochemical Analysis and Phenolic Profile of Polyfloral and Honeydew Honey from Montenegro. RSC Advances 2020, 10 (5), 2462–2471. https://doi.org/10.1039/c9ra08783d

Supplementary material for: [https://doi.org/10.1039/c9ra08783d]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3827