Application of biotechnology for the domestication of Dacryodes edulis (G. Don) H. J. Lam in Cameroon: A review

Cultivation of Dacryodes edulis (G. Don) H. J. Lam commonly known as safou to improve the livelihood of the local population has been growing rapidly in the range of occurrence of safou and recent emergence of the market chain introduced by the World Agroforestry Center (ICRAF) experts has stimulated this further. Domestication through conventional genetic improvement (breeding) of safou has relied on phenotype selection and quantitative genetics through field trials breeding. Technologies to increase productivity, increase sustainable resource use, respond to climate change and enhance efficiency are required to meet demand. Biotechnology applications give a scope for rapid improvement and also facilitate the breeding program. Advantages of biotechnology application using molecular markers in breeding programs includes: study of genetic diversity, DNA fingerprinting of individuals, easy identification of specific traits or genes of interest, rapid propagation of improved genotypes and integration of gene(s) of interest into the species. It also provides genetic basis for selecting individuals and particular regions of the genome in a breeding program, reduce breeding population, can lead to early selection of traits and the development of a new variety with combination of characteristics. The complementary role of these techniques will be necessary for a successful genetic improvement program in the species. This review examines the achievements obtained using classical techniques, emphasizes missing gaps for the application of molecular techniques and discusses the complementary role of biotechnology techniques for a sustainable genetic improvement program in the species.Keywords: Dacryodes edulis, domestication, population, genetic diversity, multiplication, biotechnology and genetic improvemen

Methodological approaches for the selection of genotypes in a progeny trial of Dacryodes edulis (G. Don) H. J. Lam in Cameroon

The World Agroforestry Centre (ICRAF) initiated a program in Cameroon for the selection of improved genotypes of Dacryodes edulis for domestication. There are major challenges in such selection because superior performance of individual trees may be based on site conditions rather than on genetic differences. Open-pollinated seeds were collected from mother trees in four provenances in Cameroon and planted in a progeny trial at Minkoameyos, Cameroon. Progeny from the same mother tree are referred to as family. After one year of growth in the field, growth traits were measured annually for five years and fruit traits were measured in two successive years. Three methodological approaches were investigated: family and within-family selection (combined selection), ranking for phenotypic selection and correlation for multi-trait and early selection. Variation in growth and fruit traits among families was not significant for most provenances, indicating that family selection would not be effective for the selection of genotypes in the trial. Phenotypic selection resulted in the retention of a small number of individuals of all families in the trial. There were strong positive correlations between fruit traits and between fruit and growth traits, while negative correlations were found between fruit traits and yield. The correlations indicate that selecting for one fruit trait may also improve the other fruit traits but reduce fruit yield. Positive and significant juvenile-mature correlations for growth were also found in this trial, indicating that early selection may be possible based ongrowth. Based on these results, phenotypic selection is recommended in the progeny trial for the domestication program in Cameroon and further research on early selection is needed as the trial advances in age

The management of tree genetic resources and the livelihoods of rural communities in the tropics: non-timber forest products, smallholder agroforestry practices and tree commodity crops

Products and services provided by trees in forests and farmland support the needs and promote the wellbeing of hundreds of millions of people in the tropics. Value depends on managing both the diversity of tree species present in landscapes and the genetic variation within these species. The benefits from trees and their genetic resources are, however, often not well quantified because trade is frequently outside formal markets, there is a multiplicity of species and ways in which trees are used and managed, and genetic diversity within species is frequently not given proper consideration. We review here what is known about the value of trees to rural communities through considering three production categories: non-timber products harvested from trees in natural and managed forests and woodlands; the various products and services obtained from a wide range of trees planted and/or retained in smallholders’ agroforestry systems; and the commercial products harvested from cultivated tree commodity crops. Where possible, we focus on the role of intra-specific genetic variation in providing support to livelihoods, and for each of the three production categories we also consider wider conservation and sustainability issues, including the linkages between categories in terms of management. Challenges to ‘conventional wisdom’ on tree resource use, value and management – such as in the posited links between commercialisation, cultivation and conservation – are highlighted, and constraints and opportunities to maintain and enhance value are described

Analyzing the Contribution of Cameroon’s Council Forests to Climate Change Mitigation and Socioeconomic Development

Council forests were officially enacted in Cameroon in 1994 as part of the forestry law reform. The law provided rural councils with the legal right to create their own forests estate within the Permanent Forest Estate (PFE) of the State, following the preparation of a management plan approved by the forest administration. In this chapter, we analyze the socioeconomic and climate change mitigation potentials of these forests and propose possible options for improving their socioeconomic importance as well as their ability to mitigate climate change. Results indicate that Cameroon’s council forests provide socioeconomic opportunities to communities in which they are located including employment and revenue from the sale of timber and nontimber forest products emanating from these forests. Additionally, given their diversity in terms of the various forest types (e.g., humid dense evergreen forests, humid dense semideciduous forests, and gallery forests), these forests have enormous carbon stocks which can provide huge opportunities for international climate initiatives such as the REDD+ mechanism to be initiated within them as a potential for mitigating global climate change. The chapter identifies and discusses possible options for improving the socioeconomic and climate change mitigation potential of these forests. Progress on the options the chapter opines, will help in improving the contributions of these forests to socioeconomic development and climate change mitigation

Analysis of genetic diversity in accessions of Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill

Amplified fragment length polymorphism (AFLP) was used to assess genetic diversity and relationships among 15 accessions of Irvingia gabonensis collected from Cameroun, Gabon, and Nigeria. Twelve AFLP+3 primers produced 384 polymorphic fragments. Average genetic distance (AGD) between the 15 accessions was 58.7% (32-88%). AGD and range of genetic distance among accessions from Cameroun, Nigeria and Gabon were 62% (53-76%), 52% (32.3 – 84.8%) and 50% (45- 53%), respectively, indicating more genetic diversity in Cameroun than Nigeria and Gabon. The unweighted pair-group method of the arithmetic average (UPGMA) and principal coordinate analysis (PCO) showed a clear distinction between the Gabon and Nigeria accessions into two separate clusters, with accessions from Cameroun overlapping them. Principal coordinate analysis (PCO) indicated a closer relationship between accessions from Cameroun and Gabon. In general the Cameroun germplasm appears to be a bridge between the genetically isolated Nigeria and Gabon accessions. This overlap of Gabon and Nigerian accessions by the accessions from Cameroun may be an indication that Cameroun is the center of diversity of I. gabonensis and also the primary source of original materials grown in the other countries. More collection in Cameroun is necessary to ensure the optimum collection and preservation of the existing genetic diversity in I. gabonensis.Keywords: Irvingia gabonensis, Accession, amplified fragment length polymorphism, genetic diversit

Medicinal potential, utilization and domestication status of bitter kola (Garcinia kola Heckel) in West and Central Africa

Garcinia kola Heckel (Clusiaceae), known as bitter kola, is a multipurpose tree indigenous to West and Central Africa. This highly preferred species is called wonder plant because all of its parts can be used as medicine. Its seeds, the most valued product of the tree, are commonly eaten to prevent/cure gastric disorders and for their typical astringent taste. There is a vast evidence that bioactive components of the seeds can serve as alternative medicine to treat/prevent severe illnesses such as malaria, hepatitis and immune-destructive diseases. Despite the species' pharmaceutical potential and its high preference by West and Central African communities, G. kola is still at the beginning of its domestication process. Even though, there are numerous scientific articles published on species biological activities, it is a difficult task to find basic information on its diversity, distribution, genetics, silvicultural management or botany. Therefore, in this very first review published on G. kola, we summarize all relevant information known about the species, target some of the challenges connected with its cultivation and propose a leading direction for future research and domestication process

Influence de la provenance du géniteur et du type de pollen sur la fructification sous pollinisation contrôlée chez Dacryodes edulis (Burseraceae) au Cameroun

Très peu de travaux ont été réalisés sur l’amélioration variétale des espèces locales. L’amélioration de leur germoplasme aboutit à l’obtention du matériel végétal amélioré destiné à la création de nouveaux cultivars. Des essais de pollinisations croisées contrôlées ont été menés sur Dacryodes edulis dans le but d’évaluer l’influence de la provenance des géniteurs et du type de pollen sur la fructification de 14 accessions femelles issues de 03 provenances. Les croisements ont été effectués selon un schéma en bloc imbriqué. Pour chaque croisement effectué, le nombre de fleurs pollinisées, le nombre de fruits noués et le nombre de fruits arrivés à maturité ont été évalués; le taux de nouaison (TN), l’indice de fructification (IF) et le taux de chute des fruits (TC) calculés et soumis à une analyse de variance. A partir des moyennes obtenues, les différentes combinaisons ont été classées en fonction de leur performance à l’aide du ‘cluster analysis’. Les résultats obtenus ont montré que l’indice de fructification qui détermine le rendement de l’espèce étudiée varie uniquement et de manière significative (p=0,010) sous l’action combinée des trois facteurs étudiés. Six meilleures combinaisons caractérisées principalement par un taux de nouaison, un taux de fructification élevés et un faible taux de chute de fruits après nouaison (plus de 70%, plus de 50% et moins de 20% respectivement) ont été identifiées. Les graines des fruits (hybrides F1) issues de cette étude ont été caractérisées et les plants ont été produits en pépinière. Ces derniers serviront de matériel de base d’un essai clonal dans un futur proche.Mots clés: Banque de gènes, essai clonal, germoplasme, safoutier, pollinisation contrôlé