Cytotoxic, antimicrobial and DNA breaking activity of Salgam

Salgam is one of the traditional fermented beverage that was produced and consumed by Turkish people. It is a sour-soft beverage which is red in colour. Therefore, this study aimed to determine the cytotoxic, antimicrobial and DNA breaking activity of Salgam. Here, the cytotoxic effect of Salgam was studied by MTT assay using K562 (human bone marrow cells) cell line. Genotoxic effect of Salgam was studied by testing the effect of the substance on supper coiled double helix DNA. In addition, antibacterial effects of Salgam were investigated using Klebsiella pneumoniae and Staphylococcus aureus strains. K562 cells were treated with Salgam concentrations of 0.3125%, 0.625%, 1.25%, 2.5%, 5% and 10% for 24 h, after that cytotoxic effect of Salgam was studied by MTT test. Three methods were used to determine the antibacterial effect of Salgam; Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Disc Diffusion Assay. DNA damaging effect of four concentrations of Salgam against pET22b circular DNA also investigated. Salgam inhibited proliferation of K526 cells at highest concentration for 24 h treatment period. It had no effect on Staphylococcus aureus and Klebsiella pneumoniae and the result of pET22 plasmid DNA breaking analysis revealed that Salgam did not affect the pET22b. Therefore, it concluded that Salgam can be considered as a safe beverage for human cells and bacterial flora

In vivo and in vitro investigation of toxicity and protective effects of 4-mei using cytogenetic and molecular techniques.

TEZ11835Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2016.Kaynakça (s. 93-107) var.xii, 107 s. : res. (bzs. rnk.), tablo ; 29 cm.Bu çalıGmada test maddesi olarak 4-metilimidazol (heterosiklik organik kimyasal bileGiği) (4-MEI) yaygın olarak insanların besinlerinde yer almaktadır. Bu çalıGmanın amacı karemelizasyon sırasında ortaya çıkan 4-metilimidazol (4-MEI)?un kanserojenik ve antikanserojenik etkilerinin olup olmadığını oluGturduğumuz kolon kanserli sıçan modellerinde K-ras ve ?-catenin genlerine ait mRNA ve proteinlerin Real Time PCR ve Western Blot teknikleri ile ekspresyon düzeyleri belirlemektir. Ayrıca bu araGtırmada 4-MEI?nın genotoksik, antigenotoksik, sitotoksik ve antisitotoksik etkileri in vivo olarak fare kemik iliğinde araGtırılmıGtır. Bunlarla birlikte 4-MEI?nın antibakteriyel etkisi Bacillus subtilis, E. coli, Pseudomonas aeruginosa ve Staphylococcus aureus türünde bakılmıGtır. Bu çalıGmadan elde edilen sonuçlar kolon kanseri oluGturulmuG sıçanlarada 4-MEI?nın beta catenin ve K-ras genleri mRNA ve proteinlerinin ekspresyon seviyelerin düGürmek suretiyle antikanser özelliğe sahip olduğu ortaya çıkmıGtır. Ayrıca 4-MEI?nın fare kemik iliğinde genotoksik ve sitotoksik etkiye sahip olduğu ve antigenetoksik etki göstermediği bu çalıGmada saptanmıGtır. Bunların yanı sıra 4-MEI?nın dört farklı bakteri türünde antibakteriyel etki gösterdiği de ortaya çıkmıGtır. Bu çalıGmadan elde edilen sonuçlar 4-MEI?nın antikanser özelliğe sahip olabileceğini ama sağlıklı hücreler için genotoksik etkili olabileceğini göstermiGtir.4-methylimidazole (heterocyclic organic chemical compound) (4-MEI) is commonly found in human diet. This research was aimed to study of carcinogenic and anti-carcinogenic effects of 4-MEI, which was arisen during caramelization. The analysis was carried out by evaluating K-ras and the ?-catenin gene expression in colon cancer rat models, made in our lab, by Real Time PCR and Western Blot techniques. Also, in this study genotoxic, antigenotoxic, cytotoxic and anti-cytotoxic effects of the 4-MEI were investigated in mice bone marrow cells. In addition, antibacterial effect of 4-MEI was examined using Bacillus subtilis, E. coli, Pseudomonas aeruginosa and Staphylococcus aureus as bacterial strains. The results of this study showed that 4-MEI had anticancer properties by reducing both the transcription and translation level of the beta- catenin and K-ras genes in cancerous rats. In addition, the genotoxic and cytotoxic effects of the 4-MEI were approved in mouse bone marrow cells and it was found that these substance had no antigenotoxic effect on these cells. Also, it was supported that the 4-MEI has anti-bacterial effect on four different types of bacteria. The result of this study showed that 4-MEI can have anticancer effect but it can also be harmful for normal cells.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FDK-2014-2617

Cytotoxic, antimicrobial and DNA breaking activity of Salgam

169-174    Salgam is one of the traditional fermented beverage that was produced and consumed by Turkish people. It is a sour-soft beverage which is red in colour. Therefore, this study aimed to determine the cytotoxic, antimicrobial and DNA breaking activity of Salgam. Here, the cytotoxic effect of Salgam was studied by MTT assay using K562 (human bone marrow cells) cell line. Genotoxic effect of Salgam was studied by testing the effect of the substance on supper coiled double helix DNA. In addition, antibacterial effects of Salgam were investigated using Klebsiella pneumoniae and Staphylococcus aureus strains.     K562 cells were treated with Salgam concentrations of 0.3125%, 0.625%, 1.25%, 2.5%, 5% and 10% for 24 h, after that cytotoxic effect of Salgam was studied by MTT test. Three methods were used to determine the antibacterial effect of Salgam; Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Disc Diffusion Assay. DNA damaging effect of four concentrations of Salgam against pET22b circular DNA also investigated.     Salgam inhibited proliferation of K526 cells at highest concentration for 24 h treatment period. It had no effect on Staphylococcus aureus and Klebsiella pneumoniae and the result of pET22 plasmid DNA breaking analysis revealed that Salgam did not affect the pET22b. Therefore, it concluded that Salgam can be considered as a safe beverage for human cells and bacterial flora.</em

Investigation of antibacterial effects of chlorzoxazone

WOS: 000480626400046Purpose: Some of non-antibiotic drugs may provide inhibition against bacterial growth in their routine use. Chlorzoxazone is a muscle relaxant used to treat muscle spasm and the resulting pain or discomfort. This study was aimed to the investigation of antibacterial effects of Chlorzoxazone using MIC (Minimum inhibitory concentration), MBC (minimum bactericidal concentration) and disk diffusion method. Materials and Methods: The MIC and MBC was determined by dilution assay using different concentrations of Chlorzoxazone against 2 bacterial strain (Pseudomonas aeruginosa as a gram negative bacterial strain and Bacillus subtilis as a gram positive bacterial strain). Disk diffusion assay were prepared at different doses (25, 50 and 100 mu g). Results: MIC values of Chlorzoxazone against Pseudomonas aeruginosa was 0.800 mg ml-1, and MBC values was 2.56 mg ml-1 and MIC values of Chlorzoxazone against Bacillus subtilis was 0.400 mg ml-1, and MBC values was 1.92 mg ml-1. The results obtained from disk diffusion assay supported that the Chlorzoxazone has not antibacterial effect on Pseudomonas aeruginosa and Bacillus subtilis. Conclusion: Although antibacterial activity of Chlorzoxazone was not found but another studies are needed to determine the probable risk of Chlorzoxazone

Assessment of in vitro genotoxic and cytotoxic effects of flurbiprofen on human cultured lymphocytes

Flurbiprofen is non-steroidal anti-inflammatory drug which is commonly used for its analgesic, antipyretic, and anti-inflammatory effects. The purpose of the study was to explore the genotoxic and cytotoxic effects of flurbiprofen in human cultured lymphocytes by sister chromatid exchange, chromosome aberration, and cytokinesis-blocked micronucleus tests. 10, 20, 30, and 40 mg/mL concentrations of flurbiprofen (solvent is DMSO) were used to treatment of human cultured lymphocytes at two different treatment periods (24 and 48 h). Flurbiprofen had no significant genotoxic effect in any of these tests. But exposing to flurbiprofen for 24 and 48 h led to significant decrease on proliferation index, mitotic index, and nuclear division index (NDI). Also, all decreases were concentration-dependent (except NDI at 24 h treatment period). Consequently, the findings of this research showed that flurbiprofen had cytotoxic effects in human blood lymphocytes

In vitro cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

Flurbiprofen (FLB) (anti-inflammatory and analgesic drug) and roxithromycin (RXM) (antibiotic) were widely used in world wide. This study deals with investigation of genotoxicity, cytotoxicity, and oxidative stress effects of a particular combination of these drugs in human cultured lymphocytes. Also, DNA damaging-protective effects of combination of these drugs were analyzed on plasmid DNA. Human lymphocytes were treated with different concentrations (FLB + RXM; 10 mg/mL + 25 mg/mL, 15 mg/mL + 50 mg/mL, and 20 mg/mL + 100 mg/mL) of the drugs following by study of their genotoxic and cytotoxic effects by analysis of cytokinesisblock micronucleus test and nuclear division index, respectively. The effect of the combination in aspect of anti-oxidative and DNA damaging activity was evaluated on Pet-22b plasmid. According to our results, the combination of FLB and RXM did not show a notable genotoxic effect on cells. Although each of the substances had been shown as a cytotoxic agent by previous researchers, in this research, the combination of these drugs did not exhibit any adverse effect on cell division. FLB had DNA protection effect against H2O2 while in combination with RXM had not the same effect on the plasmid