19 research outputs found
Adjuvant Corticosteroid Therapy in Hepatosplenic Candidiasis-Related Iris
Candida infections are the most frequent infections in neutropenic patients. Hepatosplenic candidiasis (HSC) is a part of disseminated Candida infection that occurs most commonly in patients with hematologic malignancies treated with chemotherapy and requires protracted antifungal therapy. During invasive mycosis with rapid resolution of immunosuppression, immune reconstitution inflammatory syndrome (IRIS) which mimics treatment failure, drug toxicity or breakthrough infections may occur. Manifestation period, histopathologic findings and favorable effect of steroids to its inflammatory symptoms strongly suggest that HSC belongs to the invasive fungal infection induced IRIS. We present a child with B cell-acute lymphoblastic leukemia who developed HSC and addition of corticosteroid therapy to antifungal treatment achieved rapid resolution of the clinical symptoms and laboratory findings
The Effect of Granulocyte Colony–Stimulating Factor on Immune-Modulatory Cytokines in the Bone Marrow Microenvironment and Mesenchymal Stem Cells of Healthy Donors
AbstractGranulocyte colony stimulating factor (G-CSF) is sometimes administered to donors before bone marrow (BM) harvest. G-CSF–primed (G-BM) and unprimed BM (U-BM)–derived mesenchymal stem cells (MSC) were obtained from 16 healthy donors and were expanded in vitro. Their proliferative characteristics, morphology, and differentiation capacity were examined. Supernatants of the second passage of MSCs were evaluated for transforming growth factor β1, hepatocyte growth factor, and prostaglandin E2 (PGE2) levels and compared with controls. The analyses of cytokines in the G-BM– and U-BM–derived MSCs supernatants revealed that PGE2 levels were significantly lower in the G-CSF–primed samples. These cytokines were also measured in BM plasma. The level of hepatocyte growth factor in G-BM plasma was significantly increased. The current study is the first to show the effects of G-CSF on the BM microenvironment of healthy human donors. The preliminary data suggest that G-BM– and U-BM–derived MSCs have similar morphologic/phenotypic properties and differentiation capacity but differ in their secretory capacity. Significant changes in cytokine levels of BM plasma in G-CSF–primed donors were also demonstrated. These findings suggest that BM MSCs and changes in the BM microenvironment may contribute to the effects of G-CSF on inflammation and immunomodulation
Conditioning Regimens in Allogeneic Hematopoietic Stem Cell Transplantation
Conditioning plays a central role in allogeneic hematopoietic stem cell transplantation (HSCT). The aim of the conditioning regimen in allogeneic HSCT is to prepare the patient for its transplantation. Conditioning regimen is given with three main objectives: ‘creation of space’, ‘immunosuppression’ and ‘disease eradication’. Optimal conditioning regimen will eradicate the disease, enable engraftment, and cause little toxicity for the patient. Errors in its application may have serious or even fatal consequences. Then, conditioning regimens should be carefully applied in allogeneic HSCT
Hypereosinophilic Syndrome: Hacettepe Experience.
The aim was to evaluate baseline demographic, clinical,
and laboratory characteristics, treatment modalities, and outcome
of children with idiopathic hypereosinophilic syndrome (HES)
followed up in our center. Children who fulfilled the criteria of
idiopathic HES followed up at Hacettepe University Faculty of
Medicine, Pediatric Hematology Department between June 2004
and October 2013 were included in this study. Medical records of
all children with idiopathic HES were reviewed to obtain regarding
data. The mean age of 6 children with idiopathic HES was
52.8± 44.3 months (13 to 132 mo) at diagnosis. Among 6 children
with idiopathic HES; 2 had pulmonary involvement; 1 had cardiac
and pulmonary involvement and splenomegaly; 1 had cardiac
involvement and hepatosplenomegaly; 1 had cardiac and central
nervous system involvement; and 1 had skin involvement. The
mean follow-up duration was 36.5± 31.4 months. Methyl prednisolone (MP) was used for the first-line therapy. Complete
response was achieved with MP in 3 children. All steroid responsive
children are alive; whereas 3 children who did not respond to MP
had expired. In conclusion, cardiac and pulmonary involvement is
the major causes of mortality in HES. Resistance to steroid therapy
indicates poor prognosisWo
Efficacy of hematopoietic stem cell transplantation and prophylactic triple intrathecal therapy in a child with multiple CNS relapse of acute lymphoblastic leukemia
WOS: 000322819400001PubMed ID: 2378184
The Effect of Granulocyte Colony-Stimulating Factor on Immune-Modulatory Cytokines in the Bone Marrow Microenvironment and Mesenchymal Stem Cells of Healthy Donors
Granulocyte colony stimulating factor (G-CSF) is sometimes administered to donors before bone marrow (BM) harvest. G-CSF primed (G-BM) and unprimed BM (U-BM) derived mesenchymal stem cells (MSC) were obtained from 16 healthy donors and were expanded in vitro. Their proliferative characteristics, morphology, and differentiation capacity were examined. Supernatants of the second passage of MSCs were evaluated for transforming growth factor in, hepatocyte growth factor, and prostaglandin E2 (PGE2) levels and compared with controls. The analyses of cytokines in the G-BM and U-BM derived MSCs supernatants revealed that PGE2 levels were significantly lower in the G-CSF primed samples. These cytokines were also measured in BM plasma. The level of hepatocyte growth factor in G-BM plasma was significantly increased. The current study is the first to show the effects of G-CSF on the BM microenvironment of healthy human donors. The preliminary data suggest that G-BM and U-BM derived MSCs have similar morphologic/ phenotypic properties and differentiation capacity but differ in their secretory capacity. Significant changes in cytokine levels of BM plasma in G-CSF primed donors were also demonstrated. These findings suggest that BM MSCs and changes in the BM microenvironment may contribute to the effects of G-CSF on inflammation and immunomodulation. (C) 2015 American Society for Blood and Marrow Transplantation.WoSScopu