Assessment of nutrient entry pathways and dominating hydrological processes in lowland catchments

International audienceThe achievement of a good water quality in all water bodies until 2015 is legally regulated since December 2000 for all European Union member states by the European Water Framework Directive (EU, 2000). The aim of this project is to detect nutrient entry pathways and to assess the dominating hydrological processes in complex mesoscale catchments. The investigated Treene catchment is located in Northern Germany as a part of a lowland area. Sandy, loamy and peat soils are characteristic for this area. Land use is dominated by agriculture and pasture. Drainage changed the natural water balance. In a nested approach we examined two catchment areas: a) Treene catchment 517 km2, b) Kielstau catchment 50 km2. The nested approach assists to improve the process understanding by using data of different scales. Therefore these catchments serve not only as an example but the results are transferable to other lowland catchment areas. In a first step the river basin scale model SWAT (Soil and Water Assessment Tool, Arnold et al., 1998) was used successfully to model the water balance. Furthermore the water quality was analysed to distinguish the impact of point and diffuse sources. The results show that the tributaries in the Kielstau catchment contribute high amounts of nutrients, mainly nitrate and ammonium. For the parameters nitrate, ammonium and phosphorus it was observed as a tendency that the annual loads were increasing along the river profile of the Kielstau

Effects of LatticeQCD EoS and Continuous Emission on Some ObseErvables

Effects of lattice-QCD-inspired equations of state and continuous emission on some observables are discussed, by solving a 3D hydrodynamics. The particle multiplicity as well as v2 are found to increase in the mid-rapidity. We also discuss the effects of the initial-condition fluctuations.Comment: 6 pages, 10 figures, prepared for Workshop on Particle Correlations and Fentoscopy, Kromeriz (Czech Republic), Aug. 15-17,200

Disruption of ph dynamics suppresses proliferation and potentiates doxorubicin cytotoxicity in breast cancer cells

The reverse pH gradient is a major feature associated with cancer cell reprogrammed metabolism. This phenotype is supported by increased activity of pH regulators like ATPases, carbonic anhydrases (CAs), monocarboxylate transporters (MCTs) and sodium–proton exchangers (NHEs) that induce an acidic tumor microenvironment, responsible for the cancer acid-resistant phenotype. In this work, we analyzed the expression of these pH regulators and explored their inhibition in breast cancer cells as a strategy to enhance the sensitivity to chemotherapy. Expression of the different pH regulators was evaluated by immunofluorescence and Western blot in two breast cancer cell lines (MDA-MB-231 and MCF-7) and by immunohistochemistry in human breast cancer tissues. Cell viability, migration and invasion were evaluated upon exposure to the pH regulator inhibitors (PRIs) concanamycin-A, cariporide, acetazolamide and cyano-4-hydroxycinnamate. Additionally, PRIs were combined with doxorubicin to analyze the effect of cell pH dynamic disruption on doxorubicin sensitivity. Both cancer cell lines expressed all pH regulators, except for MCT1 and CAXII, only expressed in MCF-7 cells. There was higher plasma membrane expression of the pH regulators in human breast cancer tissues than in normal breast epithelium. Additionally, pH regulator expression was significantly associated with different molecular subtypes of breast cancer. pH regulator inhibition decreased cancer cell aggressiveness, with a higher effect in MDA-MB-231. A synergistic inhibitory effect was observed when PRIs were combined with doxorubicin in the breast cancer cell line viability. Our results support proton dynamic disruption as a breast cancer antitumor strategy and the use of PRIs to boost the activity of conventional therapy.This research was funded by National funds, through the Foundation for Science and Technology (FCT) - project UIDB/50026/2020 and UIDP/50026/2020; and by the projects NORTE-01-0145-FEDER-000013 and NORTE-01-0145-FEDER-000023, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work was also supported by an internal CESPU project MetabRes_CESPU_2017. DT-V received a fellowship from FCT (ref. SFRH/BD/103025/2014)

Bilepton effects on the WWV^* vertex in the 331 model with right-handed neutrinos via a SU_L(2)XU_Y(1) covariant quantization scheme

In a recent paper, we investigated the effects of the massive charged gauge bosons (bileptons) predicted by the minimal 331 model on the off-shell vertex WWV^* (V=gamma, Z) using a SU_L(2) X U_Y(1) covariant gauge-fixing term for the bileptons. We proceed along the same lines and calculate the effects of the gauge bosons predicted by the 331 model with right-handed neutrinos. It is found that the bilepton effects on the WWV^* vertex are of the same order of magnitude than those arising from the SM and several of its extensions, provided that the bilepton mass is of the order of a few hundred of GeVs. For heavier bileptons, their effects on the WWV^* vertex are negligible. The behavior of the form factors at high energies is also discussed as it is a reflect of the gauge invariance and gauge independence of the WWV^* Green function obtained via our quantization method.Comment: Replaced to match published versio

Magentically-Induced Lattice Distortions and Ferroelectricity in Magnetoelectric GdMnO3

In this work we investigate the magnetic field dependence of Ag octahedra rotation (tilt) and B2g symmetric stretching modes frequency at different temperatures. Our field-dependent Raman investigation at 10K is interpreted by an ionic displacive nature of the magnetically induced ferroelectric phase transition. The frequency change of the Ag tilt is in agreement with the stabilization of the Mn-Gd spin arrangement, yielding the necessary conditions for the onset of ferroelectricity on the basis of the inverse Dzyaloshinskii-Moriya interaction. The role of the Jahn-Teller cooperative interaction is also evidenced by the change of the B2g mode frequency at the ferroelectric phase transition. This frequency change allows estimating the shift of the oxygen position at the ferroelectric phase transition and the corresponding spontaneous polarization of 480 {\mu}C/m2, which agrees with earlier reported values in single crystals. Our study also confirms the existence of a large magnetic hysteresis at the lowest temperatures, which is a manifestation of magnetrostiction.Comment: 5 pages, 3 figure

Construção de Terra: Projecto de Andorinha

O presente trabalho de investigação tem como principal objectivo dar um contributo na temática das soluções de melhoramento do comportamento e do reforço de elementos construtivos de terra a partir do estudo biomimético do ninho da andorinha-dos-beirais. Os resultados experimentais obtidos apontam para a existência de polissacarídeos/açucares no material do ninho e que possívelmente são adicionados pela andorinha-dos-beirais durante o processo de construção do ninho

Goat's milk allergy

BACKGROUND: Goat's milk (GM) allergy not associated with allergy to cow's milk (CM) is a rare disorder. Caseins have been implicated has the major allergens eliciting symptoms. METHODS: We report the case of a 27 years-old female patient that experienced two episodes of urticaria related to ingestion of goat's cheese (GC). She tolerated CM, dairy products and sheep cheese. Skin prick tests were performed with GM, CM, bovine casein and alpha -lactalbumin and fresh milk and GC. Serum specific IgE to GM, CM and its fractions, and GM and CM immunobloting assays with inhibition were also evaluated. RESULTS: Skin tests were positive to GM and GC and negative to CM. GM immunoblot showed an IgE-binding 14 kDa band that was totally inhibited after serum pre-incubation with GM. CONCLUSIONS: Allergens other than casein can be involved in allergy to GM. Even small quantities of protein can elicit symptoms