776 research outputs found
Surface-Dependent Mechanism for Transcellular Permeation of Polymeric Nanoparticles Across Intestinal Monolayers
Design and User Satisfaction of Interactive Maps for Visually Impaired People
Multimodal interactive maps are a solution for presenting spatial information
to visually impaired people. In this paper, we present an interactive
multimodal map prototype that is based on a tactile paper map, a multi-touch
screen and audio output. We first describe the different steps for designing an
interactive map: drawing and printing the tactile paper map, choice of
multi-touch technology, interaction technologies and the software architecture.
Then we describe the method used to assess user satisfaction. We provide data
showing that an interactive map - although based on a unique, elementary,
double tap interaction - has been met with a high level of user satisfaction.
Interestingly, satisfaction is independent of a user's age, previous visual
experience or Braille experience. This prototype will be used as a platform to
design advanced interactions for spatial learning
Conformational studies of a synthetic peptide corresponding to the repeat motif of C hordein
Structural basis for the RING catalyzed synthesis of K63 linked ubiquitin chains
This work was supported by grants from Cancer Research UK (C434/A13067), the Wellcome Trust (098391/Z/12/Z) and Biotechnology and Biological Sciences Research Council (BB/J016004/1).The RING E3 ligase catalysed formation of lysine 63 linked ubiquitin chains by the Ube2V2–Ubc13 E2 complex is required for many important biological processes. Here we report the structure of the RING domain dimer of rat RNF4 in complex with a human Ubc13~Ub conjugate and Ube2V2. The structure has captured Ube2V2 bound to the acceptor (priming) ubiquitin with Lys63 in a position that could lead to attack on the linkage between the donor (second) ubiquitin and Ubc13 that is held in the active “folded back” conformation by the RING domain of RNF4. The interfaces identified in the structure were verified by in vitro ubiquitination assays of site directed mutants. This represents the first view of the synthesis of Lys63 linked ubiquitin chains in which both substrate ubiquitin and ubiquitin-loaded E2 are juxtaposed to allow E3 ligase mediated catalysis.PostprintPeer reviewe
A PAC Guide to Peer Research and Social Action on Issues Related to Youth Violence: Learning from the Peer Action Collective
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