Anthropometric, biochemical, dietary, morbidity and well-being assessments in women and children in Indonesia, India and Senegal : A UKRI GCRF Action Against Stunting Hub protocol paper

HD-K and EF were responsible for the overall design, training and overseeing implementation of the research. UF, MKH, BK, BF, RM, RPullakhandam, RPalika, TD, SFR, SD, RPradeilles, SA, AW, JPW, PH and CH were involved in its design. UF, MKH, BK, BF, DY, DS, NLZ, TCA, RM, RPullakhandam, RPalika, TD, SFR, SKB KS, DPP, DY, SD, PL-S, BD, PM, SF, ID, AD, TDVI, FT, AD, SS, BMK and DTT implemented the research. HD-K and EF wrote the manuscript. All authors read, provided comments on and approved the final version of the manuscript.Peer reviewe

Anthropometric, biochemical, dietary, morbidity and well-being assessments in women and children in Indonesia, India and Senegal: a UKRI GCRF Action Against Stunting Hub protocol paper.

INTRODUCTION: Child stunting has a complex aetiology, especially in the first 1000 days of life. Nutrition interventions alone have not produced expected impacts in reducing/preventing child stunting, indicating the importance of understanding the complex interplay between environmental, physiological and psychological factors influencing child nutritional status. This study will investigate maternal and child nutrition, health and well-being status and associated factors through the assessment of: (1) anthropometry, (2) biomarkers of nutrition and health status, (3) dietary intakes, (4) fetal growth and development, (5) infant morbidity, (6) infant and young child feeding (IYCF) and (7) perinatal maternal stress, depression and social support. METHODS: This study will be conducted in a prospective pregnancy cohort in India, Indonesia and Senegal. Pregnant women will be recruited in the second (Indonesia, Senegal) and third (India) trimester of pregnancy, and the mother and infant dyads followed until the infant is 24 months of age. During pregnancy, anthropometric measures will be taken, venous blood samples will be collected for biochemical assessment of nutrition and health status, dietary intakes will be assessed using a 4-pass-24-hour dietary recall method (MP24HR), fetal ultrasound for assessment of fetal growth. After birth, anthropometry measurements will be taken, venous blood samples will be collected, MP24HR will be conducted, infant morbidity and IYCF practices will be assessed and a sample of breastmilk will be collected for nutrient composition analyses. Perinatal maternal stress, depression, social support and hair cortisol levels (stress) will be measured. The results from this study will be integrated in an interdisciplinary analysis to examine factors influencing infant growth and inform global efforts in reducing child stunting. ETHICS AND DISSEMINATION: Ethical approval was granted by the Ethics Committee of the London School of Hygiene and Tropical Medicine (17915/RR/17513); National Institute of Nutrition (ICMR)-Ministry of Health and Family Welfare, Government of India (CR/04/I/2021); Health Research Ethics Committee, University of Indonesia and Cipto Mangunkusumo Hospital (KET-887/UN2.F1/ETIK/PPM.00.02/2019); and the Comité National d'Ethique pour la Recherche en Santé, Senegal (Protocole SEN19/78); the Royal Veterinary College (URN SR2020-0197) and the International Livestock Research Institute Institutional Research Ethics Committee (ILRI-IREC2020-33). Results will be published in peer-reviewed journals and disseminated to policy-makers and participating communities

Development and Validation of A Stability Indicating Method for the Simultaneous Determination of Levosulpiride and Rabeprazole by High Performance Liquid Chromatography

A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the simultaneous estimation of Levosulpiride and Rabeprazole in pharmaceutical Tablet dosage form. The mobile phase consisted of 60:40 % (v/v) of Methanol & 0.1% v/v orthophosphoric acid operated on isocratic mode. The flow rate is 1.0 ml/min. Chromatographic separation of Levosulpiride and Rabeprazole was performed on AGILENT POLARIS C18 column (150 X 4.6 mm id, ODS 2, 5?m). The wavelength of detection is 232 nm. The injection volume is 20?L. The retention time of Levosulpiride and Rabeprazole are 2.1 ± 0.10 minutes and 4.1 ± 0.10 respectively. The run time of analysis is 6 minutes. The developed method was validated for parameters such as accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The influence of acid, alkaline, oxidative Stress and photolytic stress conditions on both the drugs was studied. Results indicated complete degradation in alkaline medium for Levosulpiride and Rabeprazole. The proposed method has been successfully used for the estimation in tablet dosage forms

Development and Validation of A Stability Indicating Method for the Simultaneous Determination of Atenolol and Hydrochlorothiazide by HPLC

A simple, selective, rapid, precise and stability indicating high performance liquid chromatographic method was developed and validated for the simultaneous estimation of Atenolol and Hydrochlorothiazide in pharmaceutical Tablet dosage form. Materials and Methods: The method involves the use of easily available inexpensive laboratory reagents.  The mobile phase consisted of 50:50 % (v/v) of Methanol & 0.1% v/v orthophosphoric acid operated on isocratic mode. The flow rate is 0.6 ml/min. Chromatographic separation of  Atenolol and Hydhrochlorothiazide was performed on Phenomenox Prodigy C18 column (150 X 4.6 mm id, ODS 2, 5µm). The wavelength of detection is 271 nm. The injection volume is 20µL. The retention time of Atenolol and Hydhrochlorothiazide are 2.68 ± 0.10 minutes and 3.49 ± 0.10 respectively. The run time of analysis is 5 minutes. Results: A linear response was observed over the concentration range 10.00-100.00 ?g/mL of Atenolol and the concentration range 1.00-10.00 ?g/mL of Hydrochlorothiazide. Limit of detection and limit of quantitation for Atenolol were 0.1?g/mL and 1 ?g/mL, and for Hydrochlorothiazide were 0.05 ?g/mL and 0.5 ?g/mL, respectively. The method was successfully validated in accordance to ICH guidelines acceptance criteria for linearity, accuracy, precision, specificity, robustness. The influence of acid, alkaline, oxidative Stress and photolytic stress conditions on both the drugs was studied. Results indicated complete degradation in alkaline medium for Atenolol and Hydrochlorothiazide Conclusion: The analysis concluded that the method was selective for simultaneous estimation of Atenolol and Hydrochlorothiazide can be potentially used for the estimation of these drugs in combined dosage form

Development and Validation of HPLC Method for the Determination of Suvorexant in Pharmaceutical Dosage Forms

The present experiment was focused in developing an accurate and precise method for the estimation of Suvorexant in pharmaceutical Tablet dosage form. The mobile phase consisted of 65:35 % (v/v) of Methanol & 0.1% orthophosphoric acid solution operated on isocratic mode. The flow rate is 1.0 ml/min. Chromatographic determination of Suvorexant was performed on Agilent Zorbax 300oA Extend-C18 column (150 X 4.6 mm id, 5µm). The wavelength of detection is 248 nm. The injection volume is 20µL. The retention time of Suvorexant is 5.50 ± 0.10 minutes. The method was validated as per ICH guidelines and has been successfully used for the estimation in tablet dosage forms

Development, Validation & Stress Degradation Studies Of Triprolidine By Reverse Phase-high Performance Liquid Chromatographgy (Rp-hplc)

To develop a simple, selective and precise and rapid reverse phase high performance liquid chromatography method for the development and validation, stress degradation studies of Troprolidine in bulk and Pharmaceutical dosage forms. Methods: The chromatographic separations was performed by Agilent Polaris C18 column (150 X 4.6 mm id, ODS 2,5µ m) using methanol: 0.1% ortho-phosphoric acid in water (65:35) as mobile phase at flow rate of 0.1 mL/min with injection volume 20 µL and the detection was carried out using UV detector at 232nm. The method was validated as per ICH guidelines. Results: The retention time for Troprolidine was found to be 1.88 min respectively . The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range of 1.01-10.05µg/mL. The correlation coefficient and percent recovery for Troprolidine were found to be 0.9989 and 100.66 respectively. The influence of Acid, Alkaline, Oxidative stress, Photolytic stress condition on Troprolidine was studied. Results indicated completed degradation in Alkaline medium. Conclusion: The developed method was successfully validated in accordance to ICH guidelines. This method is simple, selective, linear and precise accurate and sensitive and can be used for routine analysis of bulk samples

Investigation of skin-core joints in aluminium foam sandwich panels by EDS and XPS

Al foams are used to produce aluminium foam sandwiches (AFS) panels or other mechanical parts of complex shape, which are of great interest for many industrial applications. AFS panels with a core made of AlSi10 foam and the skins of AISI 316 steel have been prepared by in situ bonding process. To optimise the process conditions, a first set of experiments has been carried out to realise samples of reduced lab scale (20 × 20 mm2), then the AFS panels of pre-industrial size (100 × 100 mm2) have been produced. The steel-foam joints were investigated by scanning electron microscopy, energy dispersion spectroscopy and X-ray photoelectron spectroscopy. The performed examinations showed that the interface formed during the foaming was characterised by a good inter-diffusion of alloying elements, testifying the excellent quality of metallurgical joints. Furthermore, the pre-industrial panels exhibited the same characteristics of lab samples; therefore, it can be concluded that the scale-up process has been implemented successfully. Copyright © 2015 John Wiley & Sons, Ltd

Development, Validation & Stress Degradation Studies Of Darunavir By Reverse Phase-high Per-formance Liquid Chromatographgy (Rp-hplc)

A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Darunavir in pharmaceutical dosage form. The mobile phase consisted of 80:20% (v/v) of Methanol & 0.1% ortho-phosphoric acid was used and operated on isocratic mode. The flow rate is 1.0 mL/min. Chromatographic determination of Darunavir was performed on Agilent Polaris C18 column (150 X 4.6 mm id, ODS 2,5µ m). The wavelength of detection is 265nm. The injection volume is 20µL. The retention time of Darunavir is 2.42 ± 0.01minutes. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability. The influence of Acid, Alkaline, Oxidative stress, Photolytic stress condition on Darunavir was studied. Results indicated completed degradation in Alkaline medium. The proposed method has been successfully used for the estimation in tablet dosage forms