Summer Abundance of Fishes in a Maine Tidal Cove with Special Reference to Temperature

The daytime abundance and localized distribution of fishes in relation to temperature were studied in a small tidal cove by beach seining on seven dates in the Back River estuary, Maine, during the summers of 1971 and 1972. Temperatures on the seven dates ranged from 15.1–26.2 C, and salinities ranged from 17.3–24.7‰. Eighteen species of fishes were captured, with mummichogs, smooth flounders, Atlantic silversides and Atlantic herring together comprising over 98% of the catch. Mummichogs and Atlantic silversides were captured primarily near the inner end of the cove, while other abundant species were caught mainly at the outer end of the cove. Several species seem well adapted to naturally warm cove temperatures. Others seem now virtually excluded because of warm temperatures. Winter flounder, Atlantic herring, and Atlantic tomcod might be excluded from the cove during daytime in summer if artificial warming of the cove were permitted

Artesunate reduces but does not prevent posttreatment transmission of Plasmodium falciparum to Anopheles gambiae.

Combination therapy that includes artemisinin derivatives cures most falciparum malaria infections. Lowering transmission by reducing gametocyte infectivity would be an additional benefit. To examine the effect of such therapy on transmission, Gambian children with Plasmodium falciparum malaria were treated with standard regimens of chloroquine or pyrimethamine-sulfadoxine alone or in combination with 1 or 3 doses of artesunate. The infectivity to mosquitoes of gametocytes in peripheral blood was determined 4 or 7 days after treatment. Infection of mosquitoes was observed in all treatment groups and was positively associated with gametocyte density. The probability of transmission was lowest in those who received pyrimethamine-sulfadoxine and 3 doses of artesunate, and it was 8-fold higher in the group that received pyrimethamine-sulfadoxine alone. Artesunate reduced posttreatment infectivity dramatically but did not abolish it completely. The study raises questions about any policy to use pyrimethamine-sulfadoxine alone as the first-line treatment for malaria

Plasmodium falciparum Antigens on the Surface of the Gametocyte-Infected Erythrocyte

BACKGROUND: The asexual blood stages of the human malaria parasite Plasmodium falciparum produce highly immunogenic polymorphic antigens that are expressed on the surface of the host cell. In contrast, few studies have examined the surface of the gametocyte-infected erythrocyte. METHODOLOGY/PRINCIPAL FINDINGS: We used flow cytometry to detect antibodies recognising the surface of live cultured erythrocytes infected with gametocytes of P. falciparum strain 3D7 in the plasma of 200 Gambian children. The majority of children had been identified as carrying gametocytes after treatment for malaria, and each donated blood for mosquito-feeding experiments. None of the plasma recognised the surface of erythrocytes infected with developmental stages of gametocytes (I-IV), but 66 of 194 (34.0%) plasma contained IgG that recognised the surface of erythrocytes infected with mature (stage V) gametocytes. Thirty-four (17.0%) of 200 plasma tested recognised erythrocytes infected with trophozoites and schizonts, but there was no association with recognition of the surface of gametocyte-infected erythrocytes (odds ratio 1.08, 95% C.I. 0.434-2.57; P = 0.851). Plasma antibodies with the ability to recognise gametocyte surface antigens (GSA) were associated with the presence of antibodies that recognise the gamete antigen Pfs 230, but not Pfs48/45. Antibodies recognising GSA were associated with donors having lower gametocyte densities 4 weeks after antimalarial treatment. CONCLUSIONS/SIGNIFICANCE: We provide evidence that GSA are distinct from antigens detected on the surface of asexual 3D7 parasites. Our findings suggest a novel strategy for the development of transmission-blocking vaccines

A mass threshold in the number density of passive galaxies at z∼\sim2

The process that quenched star formation in galaxies at intermediate and high redshift is still the subject of considerable debate. One way to investigate this puzzling issue is to study the number density of quiescent galaxies at z~2, and its dependence on mass. Here we present the results of a new study based on very deep Ks-band imaging (with the HAWK-I instrument on the VLT) of two HST CANDELS fields (the UKIDSS Ultra-deep survey (UDS) field and GOODS-South). The new HAWK-I data (taken as part of the HUGS VLT Large Program) reach detection limits of Ks>26 (AB mag). We select a sample of passively-evolving galaxies in the redshift range 1.4<z<2.5. Thanks to the depth and large area coverage of our imaging, we have been able to extend the selection of quiescent galaxies a magnitude fainter than previous analyses. Through extensive simulations we demonstrate, for the first time, that the observed turn-over in the number of quiescent galaxies at K>22 is real. This has enabled us to establish unambiguously that the number counts of quiescent galaxies at z~2 flatten and slightly decline at magnitudes fainter than Ks~22(AB mag.). We show that this trend corresponds to a stellar mass threshold $M_*10^{10.8}\,{\rm M_{\odot}}$ below which the mechanism that halts the star formation in high-redshift galaxies seems to be inefficient. Finally we compare the observed pBzK number counts with those of quiescent galaxies extracted from four different semi-analytic models. We find that none of the models provides a statistically acceptable description of the number density of quiescent galaxies at these redshifts. We conclude that the mass function of quiescent galaxies as a function of redshift continues to present a key and demanding challenge for proposed models of galaxy formation and evolution.Comment: Accepted for publication on Astronomy and Astrophysic

Non-parametric analysis of the rest-frame UV sizes and morphological disturbance amongst L* galaxies at 4<z<8

We present the results of a study investigating the sizes and morphologies of redshift 4 < z < 8 galaxies in the CANDELS GOODS-S, HUDF and HUDF parallel fields. Based on non-parametric measurements and incorporating a careful treatment of measurement biases, we quantify the typical size of galaxies at each redshift as the peak of the log-normal size distribution, rather than the arithmetic mean size. Parameterizing the evolution of galaxy half-light radius as $r_{50} \propto (1+z)^n$, we find $n = -0.20 \pm 0.26$ at bright UV-luminosities ($0.3L_{*(z=3)} < L < L_*$) and $n = -0.47 \pm 0.62$ at faint luminosities ($0.12L_* < L < 0.3L_*$). Furthermore, simulations based on artificially redshifting our z~4 galaxy sample show that we cannot reject the null hypothesis of no size evolution. We show that this result is caused by a combination of the size-dependent completeness of high-redshift galaxy samples and the underestimation of the sizes of the largest galaxies at a given epoch. To explore the evolution of galaxy morphology we first compare asymmetry measurements to those from a large sample of simulated single S\'ersic profiles, in order to robustly categorise galaxies as either `smooth' or `disturbed'. Comparing the disturbed fraction amongst bright ($M_{UV} \leq -20$) galaxies at each redshift to that obtained by artificially redshifting our z~4 galaxy sample, while carefully matching the size and UV-luminosity distributions, we find no clear evidence for evolution in galaxy morphology over the redshift interval 4 < z < 8. Therefore, based on our results, a bright ($M_{UV} \leq -20$) galaxy at z~6 is no more likely to be measured as `disturbed' than a comparable galaxy at z~4, given the current observational constraints.Comment: 29 pages, 25 figures, 4 tables, published in MNRA

Galaxies at z = 6 - 9 from the WFC3/IR imaging of the HUDF

We present the results of a systematic search for galaxies in the redshift range z = 6 - 9, within the new, deep, near-infrared imaging of the Hubble Ultra Deep Field provided by the Wide Field Camera 3 (WFC3) on HST. We have performed full SED fitting to the optical+infrared photometry of all high-redshift galaxy candidates detected at greater than 5-sigma in at least one of the WFC3/IR broad-band filters. After rejection of contaminants, the result is a sample of 49 galaxies with primary redshift solutions z > 5.9. Our sample, selected without recourse to specific colour cuts, re-selects all but the faintest one of the 16 z-drops selected by Oesch et al. (2009), recovers all 5 of the Y-drops reported by Bouwens et al. (2009), and adds a further 29 galaxy candidates, of which 12 lie beyond z = 6.3, and 4 lie beyond z = 7. We also present confidence intervals on our photometric redshift estimates, and caution that acceptable low-redshift (z < 2) solutions exist for 28 out of the 37 galaxies at z > 6.3, and for all 8 galaxy candidates at z > 7.5. Nevertheless, the very highest redshift candidates appear to be strongly clustered in the field. We derive new estimates of the ultraviolet galaxy luminosity function at z = 7 and z = 8. Where our results are most robust, at a characteristic luminosity M(1500) ~ -19.5 (AB), we find that the comoving number density of galaxies declines by a factor of ~ 2.5 between z = 6 and z = 7, and by a further factor of ~ 2 by z = 8. These results suggest that it is difficult for the observed population of high-redshift star-forming galaxies to achieve reionisation by z ~ 6 without a significant contribution from galaxies well below the detection limits, plus alterations in the escape fraction of ionising photons and/or continued vigorous star formation at z > 15.Comment: 25 Pages, Accepted for publication in MNRA

Chloroquine/Sulphadoxine-Pyrimethamine for Gambian Children with Malaria: Transmission to Mosquitoes of Multidrug-Resistant Plasmodium falciparum

OBJECTIVES: In the Gambia, chloroquine (CQ) plus sulphadoxine-pyrimethamine (SP) is the first-line antimalarial treatment. Plasmodium falciparum parasites carrying mutations associated with resistance to each of these drugs were present in 2001 but did not cause a significant loss of therapeutic efficacy among children receiving the combination CQ/SP. We measured their effect on parasite transmission to Anopheles gambiae mosquitoes. DESIGN: We conducted a single-blind, randomised, controlled trial with follow-up over 28 d. Mosquito feeding experiments were carried out 7, 10, or 14 d after treatment. SETTING: The study took place in the town of Farafenni and surrounding villages in the Gambia. PARTICIPANTS: Participants were 500 children aged 6 mo to 10 y with uncomplicated P. falciparum malaria. INTERVENTIONS: Children were randomised to receive CQ, SP, or CQ/SP. OUTCOME MEASURES: Outcomes related to transmission were determined, including posttreatment gametocyte prevalence and density. Infectiousness was assessed by membrane-feeding A. gambiae mosquitoes with blood from 70 gametocyte-positive patients. Mutations at seven loci in four genes associated with drug resistance were measured pre- and posttreatment and in the midguts of infected mosquitoes. RESULTS: After SP treatment, the infectiousness of gametocytes was delayed, compared to the other two treatment groups, despite comparable gametocyte densities. Among bloodmeal gametocytes and the midguts of infected mosquitoes, the presence of the four-locus multidrug-resistant haplotype TYRG (consisting of mutations pfcrt-76T, pfmdr1-86Y, pfdhfr-59R, and pfdhps-437G) was associated with significantly higher oocyst burdens after treatment with the combination CQ/SP. CONCLUSIONS: Parasites with a multidrug-resistant genotype had a substantial transmission advantage after CQ/SP treatment but did not have a significant impact on in vivo efficacy of this drug combination. Protocols that include measuring transmission endpoints as well as therapeutic outcomes may be a useful strategy when monitoring the evolution of drug resistance in malaria parasites in vivo

Randomised Trial of Chloroquine/Sulphadoxine-Pyrimethamine in Gambian Children with Malaria: Impact against Multidrug-Resistant P. falciparum

OBJECTIVES: In the Gambia, the combination of chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) has replaced CQ monotherapy for treatment of malaria caused by Plasmodium falciparum. We measured the efficacy of the combination CQ/SP, and the prevalence of parasites carrying alleles associated with resistance to CQ or SP. DESIGN: We conducted a single-blind, randomised, controlled trial to compare the efficacy of CQ/SP to that of SP or CQ alone. SETTING: The study took place in the town of Farafenni and surrounding villages in the Gambia. PARTICIPANTS: Participants were children aged 12 mo to 10 y presenting as outpatients with uncomplicated P. falciparum malaria. INTERVENTIONS: 500 children were randomised to receive CQ, SP, or CQ/SP as supervised treatment and actively followed over 28 d. OUTCOME MEASURES: Primary outcome was parasitaemia at any time during follow-up. Secondary outcomes were PCR-confirmed recrudescent infections among treatment failures, and clinical failure requiring rescue medication by day 28. Pretreatment parasite isolates from 161 patients were tested for the presence of resistance-associated genetic markers. RESULTS: The prevalence of parasitological failure by day 28 for the CQ group was 60.3%, compared to 17.6% for SP (odds ratio [OR], 0.106; 95% confidence interval [CI], 0.057–0.194; p < 0.001) and 13.9% for CQ/SP (OR versus CQ, 0.140; 95% CI, 0.078–0.250; p < 0.001). There was no difference between the SP and CQ/SP groups (OR, 1.324; 95% CI, 0.705–2.50). The projected prevalence of PCR-corrected treatment failure was 30.2, 6.06, and 3.94% in the CQ, SP, and CQ/SP groups, respectively. The pfdhfr-triple mutant and pfdhps-437G mutation were common, with prevalences of 67.4 and 51.2%, respectively. Pretreatment carriage of pfdhps-437G and of multidrug-resistant parasite genotypes was associated with treatment failure in the SP group, but not in the CQ or CQ/SP groups. CONCLUSIONS: The combination of CQ/SP was an efficacious treatment for uncomplicated malaria in Gambian children in this study, but the frequent occurrence of multidrug-resistant parasites suggests that this observed efficacy is not sustainable