Schistosoma mansoni antigens alter activation markers and cytokine profile in lymphocytes of patients with asthma

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-03-14T14:02:42Z No. of bitstreams: 1 Almeida TVS Scgistosoma mansoni antigens....pdf: 2217438 bytes, checksum: 7ec6c80f61a253f1cf85fedd25c91066 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-03-14T14:18:50Z (GMT) No. of bitstreams: 1 Almeida TVS Scgistosoma mansoni antigens....pdf: 2217438 bytes, checksum: 7ec6c80f61a253f1cf85fedd25c91066 (MD5)Made available in DSpace on 2017-03-14T14:18:50Z (GMT). No. of bitstreams: 1 Almeida TVS Scgistosoma mansoni antigens....pdf: 2217438 bytes, checksum: 7ec6c80f61a253f1cf85fedd25c91066 (MD5) Previous issue date: 2017Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilInstituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Universidade Federal de Minas Gerias. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / UFBA. ProAR—Núcleo de Excelência em Asma. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais (INCT-DT/CNPq). Salvador, BA, Brasil / UFBA. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilAsthma is a chronic disease characterized by airway inflammation, obstruction and hyperresponsiveness. Severe asthma affects a small proportion of subjects but results in most of the morbidity, costs and mortality associated with the disease. Studies have suggested that Schistosoma mansoni infection reduces the severity of asthma and prevent atopy. Objective: We evaluated the ability of S. mansoni antigens, Sm29 and Sm29TSP-2 to modulate lymphocyteactivation status in response to the allergen of the mite Dermatophagoides pteronyssinus (Der p1) in cellcultures of individuals with asthma.Methods: Thirty four patients were enrolled in this study: seventeen patients with severe asthma (SAgroup), seventeen patients with mild asthma (MA group) and six controls with no asthma. Peripheralblood mononuclear cells (PBMC) were obtained and stimulated with Sm29 and Sm29TSP-2 in the pres-ence or absence of Der p1. The expression of surface markers and cytokines on lymphocytes was evaluatedby flow cytometry and the levels of IL-10 in the culture supernatant were determined by ELISA.Results: The addition of Sm29 and Sm29TSP-2 antigens to PBMC cultures from both groups of subjectswith asthma stimulated with Der p1 reduced the frequency of CD4+CD25lowcells whereas and increasedfrequency of CD4+CD25highpopulation was observed compared to unstimulated cultures. Moreover, cul-tures stimulated with Sm29TSP-2 showed a reduction in the frequency of T cells expressing CD69, IFN- ,TNF and TGF- in the MA group and an increase in the frequency of CD4+TSLPR+T cells in the SA group.The addition of Sm29 to the cultures reduced the frequency of CD4+CD69+and CD4+IL-5+T cells in all asth-matic groups, and reduced the frequency of CD4+T cells expressing IL-13 in the MA group. The culturesstimulated with Sm29 and Sm29TSP-2 showed an increase in the level of IL-10 in the supernatants.Conclusion: These results suggest that the addition of Sm29 and Sm29TSP-2 to the cells cultures fromsubjects with asthma reduced cell activation markers and altered the cytokine production pattern in away that can potentialy control the inflammatory response associated with asthm

Susceptibility of dendritic cells from individuals with schistosomiasis to infection by Leishmania braziliensis

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-29T13:35:52Z No. of bitstreams: 1 Lopes DM Susceptibility of dendritic cells from individuals ....pdf: 1207185 bytes, checksum: 5b05c2c10e01f670cf1edced5859b488 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-29T13:48:58Z (GMT) No. of bitstreams: 1 Lopes DM Susceptibility of dendritic cells from individuals ....pdf: 1207185 bytes, checksum: 5b05c2c10e01f670cf1edced5859b488 (MD5)Made available in DSpace on 2018-06-29T13:48:58Z (GMT). No. of bitstreams: 1 Lopes DM Susceptibility of dendritic cells from individuals ....pdf: 1207185 bytes, checksum: 5b05c2c10e01f670cf1edced5859b488 (MD5) Previous issue date: 2018CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: U01 AI136032.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública, Salvador, Bahia, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilTulane University School of Medicine. New Orleans, LA, USAUniversidade Federal da Bahia. Núcleo de Excelência em Asma. Salvador, Bahia, BrazilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilCoinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes from patients with schistosomiasis and patients with cutaneous leishmaniasis. MoDCs were differentiated from peripheral blood monocytes, isolated by magnetic beads, infected with L. braziliensis, and co-cultured with autologous lymphocytes. Expression of HLA-DR, CD1a, CD83, CD80, CD86, CD40, and the IL-10 receptor (IL-10R) on MoDCs as well as CD28, CD40L, CD25, and CTLA-4 on lymphocytes were evaluated by flow cytometry. The production of the cytokines IL-10, TNF, IL-12p40, and IFN-γ were evaluated by sandwich ELISA of the culture supernatant. The infectivity evaluation was performed by light microscopy after concentration of cells by cytospin and Giemsa staining. It was observed that the frequency of MoDCs expressing CD83, CD80, and CD86 as well as the MFI of HLA-DR were smaller in the group of patients with schistosomiasis compared to the group of patients with leishmaniasis. On the other hand, the frequency of IL-10R on MoDCs was higher in patients with schistosomiasis than in patients with leishmaniasis. CD4+ and CD8+ T lymphocytes from patients with schistosomiasis presented a lower frequency of CD28 and a higher frequency of CTLA-4 compared to lymphocytes from patients with leishmaniasis. Levels of IL-10 were higher in the supernatants of co-cultures from individuals with schistosomiasis compared to those with leishmaniasis. However, levels of TNF, IL-12p40, and IFN-γ were lower in the group of individuals with schistosomiasis. Regarding the frequency of MoDCs infected by L. braziliensis after 72h in culture, it was observed that higher frequencies of cells from patients with schistosomiasis were infected compared to cells from patients with leishmaniasis. It was concluded that MoDCs from patients with schistosomiasis are more likely to be infected by L. braziliensis, possibly due to a lower degree of activation and a regulatory profile