Absence of curli in soil-persistent Escherichia coli is mediated by a C-di-GMP signaling defect and suggests evidence of biofilm-independent niche specialization

peer-reviewedEscherichia coli is commonly viewed as a gastrointestinal commensal or pathogen although an increasing body of evidence suggests that it can persist in non-host environments as well. Curli are a major component of biofilm in many enteric bacteria including E. coli and are important for adherence to different biotic and abiotic surfaces. In this study we investigated curli production in a unique collection of soil-persistent E. coli isolates and examined the role of curli formation in environmental persistence. Although most soil-persistent E. coli were curli-positive, 10% of isolates were curli-negative (17 out of 170). Curli-producing E. coli (COB583, COB585, and BW25113) displayed significantly more attachment to quartz sand than the curli-negative strains. Long-term soil survival experiments indicated that curli production was not required for long-term survival in live soil (over 110 days), as a curli-negative mutant BW25113ΔcsgB had similar survival compared to wild type BW25113. Mutations in two genes associated with c-di-GMP metabolism, dgcE and pdeR, correlated with loss of curli in eight soil-persistent strains, although this did not significantly impair their survival in soil compared to curli-positive strains. Overall, the data indicate that curli-deficient and biofilm-defective strains, that also have a defect in attachment to quartz sand, are able to reside in soil for long periods of time thus pointing to the possibility that niches may exist in the soil that can support long-term survival independently of biofilm formation

Periodic Radio Emission from the T8 Dwarf WISE J062309.94-045624.6

We present the detection of rotationally modulated, circularly polarized radio emission from the T8 brown dwarf WISE J062309.94-045624.6 between 0.9 and 2.0 GHz. We detected this high proper motion ultracool dwarf with the Australian SKA Pathfinder in $1.36$ GHz imaging data from the Rapid ASKAP Continuum Survey. We observed WISE J062309.94-045624.6 to have a time and frequency averaged Stokes I flux density of $4.17\pm0.41$ mJy beam$^{-1}$, with an absolute circular polarization fraction of $66.3\pm9.0\%$, and calculated a specific radio luminosity of $L_{\nu}\sim10^{14.8}$ erg s$^{-1}$ Hz$^{-1}$. In follow-up observations with the Australian Telescope Compact Array and MeerKAT we identified a multi-peaked pulse structure, used dynamic spectra to place a lower limit of $B>0.71$ kG on the dwarf's magnetic field, and measured a $P=1.912\pm0.005$ h periodicity which we concluded to be due to rotational modulation. The luminosity and period we measured are comparable to those of other ultracool dwarfs observed at radio wavelengths. This implies that future megahertz to gigahertz surveys, with increased cadence and improved sensitivity, are likely to detect similar or later-type dwarfs. Our detection of WISE J062309.94-045624.6 makes this dwarf the coolest and latest-type star observed to produce radio emission.Comment: Accepted for publication in ApJ Letters; 11 pages, 3 figures and 2 table

Classical Novae in the ASKAP Pilot Surveys

We present a systematic search for radio counterparts of novae using the Australian Square Kilometer Array Pathfinder (ASKAP). Our search used the Rapid ASKAP Continuum Survey, which covered the entire sky south of declination $+41^{\circ}$ ($\sim34,000$ square degrees) at a central frequency of 887.5 MHz, the Variables and Slow Transients Pilot Survey, which covered $\sim5,000$ square degrees per epoch (887.5 MHz), and other ASKAP pilot surveys, which covered $\sim200-2000$ square degrees with 2-12 hour integration times. We crossmatched radio sources found in these surveys over a two-year period, from April 2019 to August 2021, with 440 previously identified optical novae, and found radio counterparts for four novae: V5668 Sgr, V1369 Cen, YZ Ret, and RR Tel. Follow-up observations with the Australian Telescope Compact Array confirm the ejecta thinning across all observed bands with spectral analysis indicative of synchrotron emission in V1369 Cen and YZ Ret. Our light-curve fit with the Hubble Flow model yields a value of $1.65\pm 0.17 \times 10^{-4} \rm \:M_\odot$ for the mass ejected in V1369 Cen. We also derive a peak surface brightness temperature of $250\pm80$ K for YZ Ret. Using Hubble Flow model simulated radio lightcurves for novae, we demonstrate that with a 5$\sigma$ sensitivity limit of 1.5 mJy in 15-min survey observations, we can detect radio emission up to a distance of 4 kpc if ejecta mass is in the range $10^{-3}\rm \:M_\odot$, and upto 1 kpc if ejecta mass is in the range $10^{-5}-10^{-3}\rm \:M_\odot$. Our study highlights ASKAP's ability to contribute to future radio observations for novae within a distance of 1 kpc hosted on white dwarfs with masses $0.4-1.25\:\rm M_\odot$ , and within a distance of 4 kpc hosted on white dwarfs with masses $0.4-1.0\:\rm M_\odot$.Comment: This paper has been accepted for publication in PASA. It consists of 13 pages, 5 figures and 4 table

Selective serotonin reuptake inhibitors and breast cancer mortality in women receiving tamoxifen: a population based cohort study

Objective To characterise whether some selective serotonin reuptake inhibitor (SSRI) antidepressants reduce tamoxifen’s effectiveness by inhibiting its bioactivation by cytochrome P450 2D6 (CYP2D6)

A pilot ASKAP survey for radio transients towards the Galactic Centre

We present the results of a radio transient and polarisation survey towards the Galactic Centre, conducted as part of the Australian Square Kilometre Array Pathfinder Variables and Slow Transients pilot survey. The survey region consisted of five fields covering $\sim265\,{\rm deg}^2$ ($350^\circ\lesssim l\lesssim10^\circ$, $\vert b\vert \lesssim 10^\circ$). Each field was observed for 12\,minutes, with between 7 and 9 repeats on cadences of between one day and four months. We detected eight highly variable sources and seven highly circularly-polarised sources (14 unique sources in total). Seven of these sources are known pulsars including the rotating radio transient PSR~J1739--2521 and the eclipsing pulsar PSR~J1723--2837. One of them is a low mass X-ray binary, 4U 1758--25. Three of them are coincident with optical or infrared sources and are likely to be stars. The remaining three may be related to the class of Galactic Centre Radio Transients (including a highly likely one, VAST~J173608.2--321634, that has been reported previously), although this class is not yet understood. In the coming years, we expect to detect $\sim$40 bursts from this kind of source with the proposed four-year VAST survey if the distribution of the source is isotropic over the Galactic fields.Comment: 17 pages, 13 figures, Accepted for publication in MNRA

Discovery of radio eclipses from 4FGL J1646.5−-4406: a new candidate redback pulsar binary

Large widefield surveys make possible the serendipitous discovery of rare sub-classes of pulsars. One such class are "spider"-type pulsar binaries, comprised of a pulsar in a compact orbit with a low-mass (sub)stellar companion. In a search for circularly-polarized radio sources in ASKAP Pilot Survey observations, we discovered highly variable and circularly polarized emission from a radio source within the error region of the $\gamma$-ray source {4FGL}~J1646.5$-$4406. The variability is consistent with the eclipse of a compact, steep-spectrum source behind ablated material from a companion in a $\sim 5.3\,$h binary orbit. Based on the eclipse properties and spatial coincidence with {4FGL} J1646.5$-$4406, we argue that the source is likely a recycled pulsar in a "redback" binary system. Using properties of the eclipses from ASKAP and Murchison Widefield Array observations, we provide broad constraints on the properties of the eclipse medium. We identified a potential optical/infra-red counterpart in archival data consistent with a variable low-mass star. Using the Parkes Radio Telescope "Murriyang" and MeerKAT, we searched extensively for radio pulsations but yielded no viable detections of pulsed emission. We suggest that the non-detection of pulses is due to scattering in the intra-binary material, but scattering from the ISM can also plausibly explain the pulse non-detections if the interstellar dispersion measure exceeds $\sim$600$\,$pc$\,$cm$^{-3}$. Orbital constraints derived from optical observations of the counterpart would be highly valuable for future $\gamma$-ray pulsation searches, which may confirm the source nature as a pulsar.Comment: Submitted to MNRAS. 13 Pages, 10 figures, 3 table

Radio Variable and Transient Sources on Minute Timescales in the ASKAP Pilot Surveys

We present results from a radio survey for variable and transient sources on 15-min timescales, using the Australian SKA Pathfinder (ASKAP) pilot surveys. The pilot surveys consist of 505 h of observations conducted at around 1 GHz observing frequency, with a total sky coverage of 1476 deg$^2$. Each observation was tracked for approximately 8-10h, with a typical rms sensitivity of $\sim$30 $\mu$jy/beam and an angular resolution of $\sim$12 arcsec. The variability search was conducted within each 8-10h observation on a 15-min timescale. We detected 38 variable and transient sources. Seven of them are known pulsars, including an eclipsing millisecond pulsar, PSR J2039$-$5617. Another eight sources are stars, only one of which has been previously identified as a radio star. For the remaining 23 objects, 22 are associated with active galactic nuclei or galaxies (including the five intra-hour variables that have been reported previously), and their variations are caused by discrete, local plasma screens. The remaining source has no multi-wavelength counterparts and is therefore yet to be identified. This is the first large-scale radio survey for variables and transient sources on minute timescales at a sub-mJy sensitivity level. We expect to discover $\sim$1 highly variable source per day using the same technique on the full ASKAP surveys.Comment: 20 pages, 11 figures; accepted for publication in MNRA

A search for radio afterglows from gamma-ray bursts with the Australian Square Kilometre Array Pathfinder

We present a search for radio afterglows from long gamma-ray bursts using the Australian Square Kilometre Array Pathfinder (ASKAP). Our search used the Rapid ASKAP Continuum Survey, covering the entire celestial sphere south of declination $+41^\circ$, and three epochs of the Variables and Slow Transients Pilot Survey (Phase 1), covering $\sim 5,000$ square degrees per epoch. The observations we used from these surveys spanned a nine-month period from 2019 April 21 to 2020 January 11. We crossmatched radio sources found in these surveys with 779 well-localised (to $\leq 15''$) long gamma-ray bursts occurring after 2004 and determined whether the associations were more likely afterglow- or host-related through the analysis of optical images. In our search, we detected one radio afterglow candidate associated with GRB 171205A, a local low-luminosity gamma-ray burst with a supernova counterpart SN 2017iuk, in an ASKAP observation 511 days post-burst. We confirmed this detection with further observations of the radio afterglow using the Australia Telescope Compact Array at 859 days and 884 days post-burst. Combining this data with archival data from early-time radio observations, we showed the evolution of the radio spectral energy distribution alone could reveal clear signatures of a wind-like circumburst medium for the burst. Finally, we derived semi-analytical estimates for the microphysical shock parameters of the burst: electron power-law index $p = 2.84$, normalised wind-density parameter $A_* = 3$, fractional energy in electrons $\epsilon_{e} = 0.3$, and fractional energy in magnetic fields $\epsilon_{B} = 0.0002$.Comment: 18 pages, 7 figures; accepted for publication in MNRA

Flow and retreat of the Late Quaternary Pine Island-Thwaites palaeo-ice stream, West Antarctica

Multibeam swath bathymetry and sub-bottom profiler data are used to establish constraints on the flow and retreat history of a major palaeo-ice stream that carried the combined discharge from the parts of the West Antarctic Ice Sheet now occupied by the Pine Island and Thwaites glacier basins. Sets of highly elongated bedforms show that, at the last glacial maximum, the route of the Pine Island-Thwaites palaeo-ice stream arced north-northeast following a prominent cross-shelf trough. In this area, the grounding line advanced to within similar to 68 km of, and probably reached, the shelf edge. Minimum ice thickness is estimated at 715 m on the outer shelf, and we estimate a minimum ice discharge of similar to 108 km(3) yr(-1) assuming velocities similar to today's Pine Island glacier (similar to 2.5 km yr(-1)). Additional bed forms observed in a trough northwest of Pine Island Bay likely formed via diachronous ice flows across the outer shelf and demonstrate switching ice stream behavior. The "style" of ice retreat is also evident in five grounding zone wedges, which suggest episodic deglaciation characterized by halts in grounding line migration up-trough. Stillstands occurred in association with changes in ice bed gradient, and phases of inferred rapid retreat correlate to higher bed slopes, supporting theoretical studies that show bed geometry as a control on ice margin recession. However, estimates that individual wedges could have formed within several centuries still imply a relatively rapid overall retreat. Our findings show that the ice stream channeled a substantial fraction of West Antarctica's discharge in the past, just as the Pine Island and Thwaites glaciers do today