Genetic variation among pathotypes of Verticillium dahliae Kleb. from cotton in western Turkey revealed by AFLP

WOS: 000327295000009Cotton (Gossypium hirsutum L.) is crucial for the textile industry worldwide. Among the diseases attacking cotton, Verticillium wilt caused by Verticillium dahliae Kleb. is the most significant. Isolates of V. dahliae can be classified into defoliating and non-defoliating pathotypes. Thirty-two isolates of the non-defoliating pathotype and one isolate of a virulent, defoliating pathotype were analysed by the amplified fragment length polymorphism (AFLP) method. Three hundred and forty AFLP fragments were obtained with nine primer combinations. The number of total bands per primer pair ranged from 16 to 81, with an average of 37.7. The average polymorphism information content (PIC) value for the AFLP products was 0.50. Using the genotypic data, genetic distance analysis was performed. The maximum variation was found between isolates (Vd11) Nazilli and (Vd16) Soke, at a value of 0.79 and the minimum variation was found between isolates (Vd20) Aydin and (Vd14) Soke, at 0.24. The unweighted paired group method with arithmetic averages cluster analysis (UPGMA) was used to discriminate the V. dahliae isolates into five subgroups. Defoliating pathotypes (Vd33) from Soke province formed a single subgroup. As a result, it was found that there was significant variation among Verticillium isolates. AFLP analysis is an efficient and effective marker technology for determining genetic relationships among Verticillium isolates

Potentiometric titrations of some mono methyl substituted and 5-keto mono methyl substituted hexadecanoic acids in nonaqueous media

1300-130

CRISPR-Of-Things%253A Applications and Challenges of the Most Popular Gene Editing Tool in the Fields of Health, Agriculture and Environment

Almost all cells of any living organism contain DNA, a hereditary molecule that passes from generation to generation during reproduction. The term quot%253Bgenomequot%253B generally refers to the total DNA sequences in an organism. The genome consists of DNA sequences called gene, which plays a role in the basic biological processes involved in many phenotypic and genotypic characteristics, such as performing cellular functions, controlling numbers and species, regulating energy production, metabolism, and combating diseases. Gene editing is the process of pre-designing and modifying a particular DNA sequence in a targeted gene. The most widely used technique is CRISPR-Cas technology. For this purpose, the DNA helix is ​​cut at a certain point, to form a double-strand break (DSB), and naturally existing cellular repair mechanisms repair the DSB. Modes of the repair mechanisms may affect the gene function. When DSB is formed, gene editing techniques can be applied to remove, insert, or replace a newly modified sequence using a synthetic donor template DNA. In developed and developing countries, CRISPR-Cas studies in addition to research and development studies are rapidly increasing. In addition to increasing population, changing weather conditions, declining farmland, increasing biotic and abiotic stresses are other important barriers to agricultural production, food, and feed supply. In this report, CRISPR-Cas applications are introduced in detail from the studies that carried out gene modifications in the fields of health, animals, plants, microorganisms, and food supply. Besides, these technologies and applications have been examined in terms of world biosafety legislation and the scientific risk assessment of the products developed using the CRISPR-Cas technique

Genomics-assisted breeding in four major pulse crops of developing countries: present status and prospects

The global population is continuously increasing and is expected to reach nine billion by 2050. This huge population pressure will lead to severe shortage of food, natural resources and arable land. Such an alarming situation is most likely to arise in developing countries due to increase in the proportion of people suffering from protein and micronutrient malnutrition. Pulses being a primary and affordable source of proteins and minerals play a key role in alleviating the protein calorie malnutrition, micronutrient deficiencies and other undernourishment-related issues. Additionally, pulses are a vital source of livelihood generation for millions of resource-poor farmers practising agriculture in the semi-arid and sub-tropical regions. Limited success achieved through conventional breeding so far in most of the pulse crops will not be enough to feed the ever increasing population. In this context, genomics-assisted breeding (GAB) holds promise in enhancing the genetic gains. Though pulses have long been considered as orphan crops, recent advances in the area of pulse genomics are noteworthy, e.g. discovery of genome-wide genetic markers, high-throughput genotyping and sequencing platforms, high-density genetic linkage/QTL maps and, more importantly, the availability of whole-genome sequence. With genome sequence in hand, there is a great scope to apply genome-wide methods for trait mapping using association studies and to choose desirable genotypes via genomic selection. It is anticipated that GAB will speed up the progress of genetic improvement of pulses, leading to the rapid development of cultivars with higher yield, enhanced stress tolerance and wider adaptability

Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum. vulgare subsp spontaneum) populations from west Turkey

WOS: 000183833700006Inter-Simple Sequence Repeat (ISSR) and Randomly Amplified Polymorphic DNA ( RAPD) markers were used to analyze genetic distance among H. vulgare subsp. spontaneum populations from west Turkey. Fifty-five RAPD and 10 ISSR primers were used to detect variation among sample. A total of 55 polymorphic loci were found using 65 primers. Two distinct cluster groups were clearly established among populations. The minimum variation was detected between Pinarbasi and Bornova ( GD = 0.192) populations and the maximum was found between Icmeler and Aydin populations ( GD = 0.926). As two dominant markers, RAPD and ISSRs are effective and promising marker systems for detecting genetic variation

Study of Odor

Abstract not availabl

Data for: Genome-Wide Association Studies of Ca and Mn content in the seeds of common bean (Phaseolus vulgaris L.)

The data uploaded describes SNP data

Screening of resistance genes to fusarium root rot and fusarium wilt diseases in F-3 family lines of tomato (Lycopersicon esculentum) using RAPD and CAPs markers

WOS: 000277847600001Fusarium diseases constitute most of the loss in tomato production worldwide, because it spreads on all geographic fields that it is so hard to find a place without fusarium infestation. Thus, the best way to produce tomato is developing resistant cultivars against Fusarium species. In cultivar developing, molecular marker assisted techniques replaced traditional breeding techniques which are high cost and time consuming for breeders. In this study RAPD and CAPS markers were used to screen tomato (Lycopersicon esculentum) lines against resistance genes Frl and I-2, respectively. Results showed that out of 115 plants, 42 were homozygous resistant, 38 were heterozygous resistant and 35 were homozygous susceptible. Under the light of this information, the forthcoming cultivar development studies will be carried out