The relationship with plasma calcium levels, metabolic syndrome and risk parameters in overweight and obese Turkish Women

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Giriş: Şişmanlık prevalansı tüm dünyada hızla artış göstermekte ve önemli bir halk sağlığı sorunu oluşturmaktadır. Serum kalsiyum düzeyleri ile vücut yağ miktarı arasında anlamlı ilişkiler saptanmıştır. Serum kalsiyum düzeyleri yükseldikçe beden kitle indeksi düzeyleri azalmakta, fakat insülin, total kolesterol ve trigliserid düzeyleri artış göstermektedir. Bu çalışmamızda şişman Türk kadınlarında serum kalsiyum düzeyleri ile kardiyovasküler risk faktörleri ve metabolik sendrom parametreleri arasındaki ilişki araştırılmıştır. Materyel ve Method: 4749 fazla kilolu ve şişman kadın retrospektif olarak incelendi. Bunlardan 872 fazla kilolu (BMI = 25-30 kg/m2) ve 3877 şişman (BMI > 30 kg/m2) hasta İstanbul Üniversitesi, İstanbul Tıp Fakültesi, İç Hastalıkları ABD, Şişmanlık Polikliniğinde değerlendirilmiş, antropometrik, biyokimyasal parametreler ölçüldü. Serum kalsiyum değerlerine göre hastalar üç gruba ayrılıp, parametreler tek yönlü ANOVA testi ile analiz edildi. p<0.05 anlamlı olarak kabul edildi.Introduction: Obesity prevalance is rapidly increasing all over the world and become a serious health problem. There is a significant correlation between serum calcium levels and body fat mass. It was indicated that when plasma calcium levels increase, body mass index decrease however, insulin, total cholesterol and triglycerides levels increase. In this study, it was investigated the relationship between plasma calcium levels and cardiovascular risk factors and metabolic syndrome parameters in Turkish overweight and obese female patients. Materiel and Method: 4749 overweight and obese women were evaluated retrospectively. 872 overweight (BMI = 25-30 kg/m2) and 3877 obese (BMI > 30 kg/m2) patients who applied to Istanbul University, Istanbul Faculty of Medicine, Departmant of Internal Medicine, Obesity Outpatient Clinic were evaluated, anthropometric and biochemical parameters were measured. Patients were divided 3 category according to plasma calcium levels. Groups were compared with one-way ANOVA. p<0.05 was accepted as significant

Disruption of a Novel Krüppel-like Transcription Factor p300-regulated Pathway for Insulin Biosynthesis Revealed by Studies of the c.-331 INS Mutation Found in Neonatal Diabetes Mellitus*

Krüppel-like transcription factors (KLFs) have elicited significant attention because of their regulation of essential biochemical pathways and, more recently, because of their fundamental role in the mechanisms of human diseases. Neonatal diabetes mellitus is a monogenic disorder with primary alterations in insulin secretion. We here describe a key biochemical mechanism that underlies neonatal diabetes mellitus insulin biosynthesis impairment, namely a homozygous mutation within the insulin gene (INS) promoter, c.-331C>G, which affects a novel KLF-binding site. The combination of careful expression profiling, electromobility shift assays, reporter experiments, and chromatin immunoprecipitation demonstrates that, among 16 different KLF proteins tested, KLF11 is the most reliable activator of this site. Congruently, the c.-331C>G INS mutation fails to bind KLF11, thus inhibiting activation by this transcription factor. Klf11−/− mice recapitulate the disruption in insulin production and blood levels observed in patients. Thus, these data demonstrate an important role for KLF11 in the regulation of INS transcription via the novel c.-331 KLF site. Lastly, our screening data raised the possibility that other members of the KLF family may also regulate this promoter under distinct, yet unidentified, cellular contexts. Collectively, this study underscores a key role for KLF proteins in biochemical mechanisms of human diseases, in particular, early infancy onset diabetes mellitus