6 research outputs found
Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling
Receptor-interacting protein kinase 3 (RIP3) is a convergence point of multiple signalling pathways, including necroptosis, inflammation and oxidative stress; however, it is completely unknown whether it underlies acute myocardial ischemia/reperfusion (I/R) injury. Langendorff-perfused rat hearts subjected to 30 min ischemia followed by 10 min reperfusion exhibited compromised cardiac function which was not abrogated by pharmacological intervention of RIP3 inhibition. An immunoblotting analysis revealed that the detrimental effects of I/R were unlikely mediated by necroptotic cell death, since neither the canonical RIP3–MLKL pathway (mixed lineage kinase-like pseudokinase) nor the proposed non-canonical molecular axes involving CaMKIIδ–mPTP (calcium/calmodulin-dependent protein kinase IIδ–mitochondrial permeability transition pore), PGAM5–Drp1 (phosphoglycerate mutase 5–dynamin-related protein 1) and JNK–BNIP3 (c-Jun N-terminal kinase–BCL2-interacting protein 3) were activated. Similarly, we found no evidence of the involvement of NLRP3 inflammasome signalling (NOD-, LRR- and pyrin domain-containing protein 3) in such injury. RIP3 inhibition prevented the plasma membrane rupture and delayed mPTP opening which was associated with the modulation of xanthin oxidase (XO) and manganese superoxide dismutase (MnSOD). Taken together, this is the first study indicating that RIP3 regulates early reperfusion injury via oxidative stress- and mitochondrial activity-related effects, rather than cell loss due to necroptosis
Impact of maturation on myocardial response to ischemia and the effectiveness of remote preconditioning in male rats
Aging attenuates cardiac tolerance to ischemia/reperfusion (I/R) associated with defects
in protective cell signaling, however, the onset of this phenotype has not been completely investigated. This study aimed to compare changes in response to I/R and the effects of remote ischemic
preconditioning (RIPC) in the hearts of younger adult (3 months) and mature adult (6 months) male
Wistar rats, with changes in selected proteins of protective signaling. Langendorff-perfused hearts
were exposed to 30 min I/120 min R without or with prior three cycles of RIPC (pressure cuff inflation/deflation on the hind limb). Infarct size (IS), incidence of ventricular arrhythmias and recovery
of contractile function (LVDP) served as the end points. In both age groups, left ventricular tissue
samples were collected prior to ischemia (baseline) and after I/R, in non-RIPC controls and in RIPC
groups to detect selected pro-survival proteins (Western blot). Maturation did not affect post-ischemic
recovery of heart function (Left Ventricular Developed Pressure, LVDP), however, it increased IS and
arrhythmogenesis accompanied by decreased levels and activity of several pro-survival proteins and
by higher levels of pro-apoptotic proteins in the hearts of elder animals. RIPC reduced the occurrence
of reperfusion-induced ventricular arrhythmias, IS and contractile dysfunction in younger animals,
and this was preserved in the mature adults. RIPC did not increase phosphorylated protein kinase B
(p-Akt)/total Akt ratio, endothelial nitric oxide synthase (eNOS) and protein kinase Cε (PKCε) prior
to ischemia but only after I/R, while phosphorylated glycogen synthase kinase-3β (GSK3β) was
increased (inactivated) before and after ischemia in both age groups coupled with decreased levels of
pro-apoptotic markers. We assume that resistance of rat heart to I/R injury starts to already decline
during maturation, and that RIPC may represent a clinically relevant cardioprotective intervention in
the elder population
Data on necrotic and apoptotic cell death in acute myocardial ischemia/reperfusion injury: the effects of CaMKII and angiotensin AT1 receptor inhibition
Content of particular proteins indicating cellular injury due to apoptosis and necrosis has been investigated in ischemic/reperfused (IR) hearts and ischemic/reperfused hearts treated with CaMKII inhibitor and/or AT1 receptor inhibitor. This data article provides information in support of the original research article “Oxidative activation of CaMKIIδ in acute myocardial ischemia/reperfusion injury: a role of angiotensin AT1 receptor-NOX2 signaling axis” [1]. Keywords: Myocardial ischemia/reperfusion injury, CaMKII inhibition, AT1 receptor blockade, Apoptosis, Necrosi
Is Intrinsic Cardioprotection a Laboratory Phenomenon or a Clinically Relevant Tool to Salvage the Failing Heart?
Cardiovascular diseases, especially ischemic heart disease, as a leading cause of heart failure (HF) and mortality, will not reduce over the coming decades despite the progress in pharmacotherapy, interventional cardiology, and surgery. Although patients surviving acute myocardial infarction live longer, alteration of heart function will later lead to HF. Its rising incidence represents a danger, especially among the elderly, with data showing more unfavorable results among females than among males. Experiments revealed an infarct-sparing effect of ischemic “preconditioning” (IPC) as the most robust form of innate cardioprotection based on the heart’s adaptation to moderate stress, increasing its resistance to severe insults. However, translation to clinical practice is limited by technical requirements and limited time. Novel forms of adaptive interventions, such as “remote” IPC, have already been applied in patients, albeit with different effectiveness. Cardiac ischemic tolerance can also be increased by other noninvasive approaches, such as adaptation to hypoxia- or exercise-induced preconditioning. Although their molecular mechanisms are not yet fully understood, some noninvasive modalities appear to be promising novel strategies for fighting HF through targeting its numerous mechanisms. In this review, we will discuss the molecular mechanisms of heart injury and repair, as well as interventions that have potential to be used in the treatment of patients