Growth components and Yield of \u3cem\u3eBrachiaria ruziziensis\u3c/em\u3e (Congo grass) and \u3cem\u3eCentrosema pascuorum\u3c/em\u3e (Centro) Mixtures as Influenced by Mixture Ratios, Phosphorus Levels and Stage of Harvest

This study was conducted during the 2017 and 2018 rainy season at the Crop Production Research farm, Abubakar Tafawa Balewa University, Bauchi to determine the effects of plant mixture ratios in Brachiaria ruziziensis and Centrosema pascuorum (100% Sole B. ruziziensis, 75% B.R: 25% C.P, 50% B.R: 50% C.P, 25% B.R: 75% C.P and 100% Sole C. pascuorum) and phosphorus fertilizer (SSP) levels (0, 100 and 150kg/ha). The experiment was in a split-plot design with 5x3 factorial arrangement in which the mixture ratios constituted the main plots while phosphorus (SSP) levels constituted the sub treatments. Number of plants per stand ranged from 6.11 in 25% B.R: 75% C.P to 9.56 in 100% B. ruziziensis and 2.89 in 75% B.R: 25% C.P to 11.11 in 50% B.R: 50% C.P. The highest plant height for B. ruziziensis was obtained in 25% B.R: 75% C.P (38.0). Similarly, 25% B.R: 75% C.P mixture had highest plant height for C. pascuorum (45.11). However, tiller per branch per m2 ranged from 57.60 in 25% B.R: 75% C.P to 190.9 in 100 B. ruziziensis and 32.9 in 75% B.R: 25% C.P to 257.90 in 100 C. pascuorum. The mean values indicated that mixture ratios and phosphorus (SSP) levels significantly (P \u3c 0.05) increased plant height and tiller numbers in B. ruziziensis and C. pascuorum mixtures and 75% B.R: 25% C.P mixture, 150kg/ha Single Super Phosphate fertilization and sampling period at week 8 had the best plant height and tiller per branch

Effect of Taurine on Chronic Restraint Stress Induced Behavioural Deficits in Rodents

Stress is an aversive stimulus capable of altering physiological homeostasis and the ability to cope with such stressful stimuli is a crucial determinant of health and disease. Taurine (2-aminoethanesulphonic acid) constitutes about 0.1 % of body weight, it is a sulphur-containing amino acid present in virtually all cells throughout the animal kingdom. It is an important ingredient used in energy drinks. The aim of the present study is to evaluate the effects of taurine in preventing the restraint stress induced memory deficit in passive avoidance test, spatial learning, motor coordination and exploratory behaviour. Three different doses of taurine were administered (100, 200 and 400 mg/kg) to rodents subjected to chronic restraint stress (6 h/day for 21 days). The treatments were administered once daily by oral gavage. Twenty four Wistar rats were divided into four groups of six rats each also; twenty four mice were divided in to four groups of six mice each. Learning and memory in the chronic restraint-stressed Wistar rats was assessed using step down passive avoidance test, learning and memory in mice was assessed using elevated plus maze for memory, exploratory activity was assessed using hole board apparatus and motor coordination in mice was assessed using beam walk assay method. In the present study it was observed that taurine improved learning and short-time memory in chronic restraint-stressed rodents it also improved motor coordination and increased exploratory behaviour in mice. Keywords: stress, taurine, deficit, exploratory, oral gavage, memor

Effects of Stages of Growth on Dry Matter Yield and Nutrient Composition of Red Clover in the Year of Establishment in Jos, Plateau State, Nigeria

An experiment was conducted in Jos, Nigeria to evaluate the effects of stages of growth on dry matter yield and nutrient composition of Red clover (Trifolium pratense var. AberClaret) in the year of establishment. The treatments were five (5) stages of growth (5, 9, 13, 17 and 21) weeks after sowing (WAS). The five stages of growth were replicated five times in a Latin Square Design. The land was divided into twenty five plots of 3 m X 3 m each. The spacing between each plot was 1m along the rows and columns. Growth components and DM yield were measured at the various stages of growth. The forage crop was also analysed for nutrients composition. Theere was no significant difference in plant height at 17 WAS (63.75 cm) and 21 WAS (64.25 cm). However, the two stages were higher (P\u3c 0.01) compared to the other stages of growth. Leaf-to-stem ratio decreased significantly from 5WAS (1.16) to 21WAS (0.26). Forage DM yield was higher (P\u3c 0.01) at 17 WAS (3.15 t ha-1) compared to the other stages of growth. Crude protein content at 9 WAS (20.88 %) was higher (P\u3c 0.01) compared to the other stages, while 21 WAS had the lowest value of 18.11 %. Crude protein (CP) content at 9WAS (208.8 g/kg) was higher (P\u3c 0.01) compared with the other stages as 21 WAS had the lowest value of 181.1 g/kg. Crude protein content decreased from 9 to 21 WAS, while the fibre fractions increased from 9 to 21WAS. Therefore, it is recommended that the legume planted in early June on the Jos Plateau, Nigeria could be harvested at 17 WAS when the DMY is maximum and CP content is also very high to meet the requirements for ruminant animals in the tropics

Overturning established chemoselectivities : selective reduction of arenes over malonates and cyanoacetates by photoactivated organic electron donors

The prevalence of metal-based reducing reagents, including metals, metal complexes, and metal salts, has produced an empirical order of reactivity that governs our approach to chemical synthesis. However, this reactivity may be influenced by stabilization of transition states, intermediates, and products through substrate-metal bonding. This article reports that in the absence of such stabilizing interactions, established chemoselectivities can be overthrown. Thus, photoactivation of the recently developed neutral organic superelectron donor 5 selectively reduces alkyl-substituted benzene rings in the presence of activated esters and nitriles, in direct contrast to metal-based reductions, opening a new perspective on reactivity. The altered outcomes arising from the organic electron donors are attributed to selective interactions between the neutral organic donors and the arene rings of the substrates

Human macrophage foam cells degrade atherosclerotic plaques through cathepsin K mediated processes

<p>Abstract</p> <p>Background</p> <p>Proteolytic degradation of Type I Collagen by proteases may play an important role in remodeling of atherosclerotic plaques, contributing to increased risk of plaque rupture.</p> <p>The aim of the current study was to investigate whether human macrophage foam cells degrade the extracellular matrix (ECM) of atherosclerotic plaques by cathepsin K mediated processes.</p> <p>Methods</p> <p>We 1) cultured human macrophages on ECM and measured cathepsin K generated fragments of type I collagen (C-terminal fragments of Type I collagen (CTX-I) 2) investigated the presence of CTX-I in human coronary arteries and 3) finally investigated the clinical potential by measuring circulating CTX-I in women with and without radiographic evidence of aortic calcified atherosclerosis.</p> <p>Results</p> <p>Immune-histochemistry of early and advanced lesions of coronary arteries demonstrated co-localization of Cathepsin-K and CTX-I in areas of intimal hyperplasia and in shoulder regions of advanced plaques. Treatment of human monocytes with M-CSF or M-CSF+LDL generated macrophages and foam cells producing CTX-I when cultured on type I collagen enriched matrix. Circulating levels of CTX-I were not significantly different in women with aortic calcifications compared to those without.</p> <p>Conclusions</p> <p>Human macrophage foam cells degrade the atherosclerotic plaques though cathepsin K mediated processes, resulting in increase in levels of CTX-I. Serum CTX-I was not elevated in women with aortic calcification, likely due to the contribution of CTX-I from osteoclastic bone resorption which involves Cathepsin-K. The human macrophage model system may be used to identify important pathway leading to excessive proteolytic plaque remodeling and plaque rupture.</p

Anti-trypanosomal Activity of Bufonidae (Toad) Venom Crude Extract on Trypanosoma brucei brucei in Swiss Mice

Trypanosomiasis afflicts about 6 ~ 7 million people globally and to a large extent impedes livestock production in Africa. Naturally, trypanosomal parasites undergo genetic mutation and have developed resistance over a wide range of therapies. The utilization of animals and plants products has presented therapeutic potential for identifying novel anti-trypanosomal drugs. This study evaluated toad venom for anti-trypanosomal potency invivo in Swiss mice. Toads were collected from July to August 2019. The acute oral toxicity and biochemical characterization of the toad venom were determined. The experimental mice were administered various doses (130 mg/kg, 173 mg/kg and 217 mg/kg) of the toad venom crude extract and 0.75 mg/mL of Diamizan Plus standard drug for the treatment of trypanosomiasis, once daily for 3 days. The in-vivo anti-trypanosomal activity was evaluated by a curative test, after infecting the mice with Trypanosoma brucei brucei. The pre-patent period was 72 hours before treatment commenced. The overall results showed that trypanosomal load was highest in the control group while the group treated with Diamizan drug had the least trypanosomal load. As such, the mean trypanosomal load in relation to treatments showed a very high significant difference (P0.05) across treatment groups. The over 50% reduction in the trypanosomal load in the 130 mg/kg group in comparison with the control group brings to bare the anti-trypanosomal potency of the toad venom. The anti-trypanosomal activity demonstrated by the toad venom has provided basis for development of new therapeutic agents from different toad species. The study recommends further studies (both in-vivo and invitro) followed by the characterization of the active compounds present in the toad venom responsible for the anti-tyrpanosomal activity observed alongside the management and conservation of these species

Effect of Longâ Term Oral Bisphosphonates on Implant Wound Healing: Literature Review and a Case Report

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141603/1/jper0584.pd

Different measures of smoking exposure and mammographic density in postmenopausal Norwegian women: a cross-sectional study

Background: Recent cohort studies have suggested an increased risk of breast cancer with long duration of smoking, and with smoking initiation before first birth. Cigarette smoking may have both carcinogenic effects and antiestrogenic effects on the breast tissue. We decided to examine the relationship between different measures of smoking exposure and mammographic density. Methods: Lifetime smoking history was collected through interview and questionnaires among 907 postmenopausal participants in the Tromsø Mammography and Breast Cancer study. The mammograms were obtained from the governmental Norwegian Breast Cancer Screening Program. Mammograms were classified according to the percentage and absolute mammographic densities using a previously validated computerassisted method. Results:Sixty-five percent of the women reported having ever smoked cigarettes, while 34% were current smokers. After adjustment for age, age at first birth, parity, age at menopause, postmenopausal hormone therapy use, and body mass index, smoking was inversely associated with both measures of mammographic density (both trends P < 0.01). Both current smokers and former smokers had significantly lower adjusted mean percentage mammographic density compared with never smokers (P = 0.003 and P = 0.006, respectively). An inverse dose–response relationship with mammographic density was found between both the number of cigarettes and the number of pack-years smoked among current smokers. Current smokers who smoked 11 cigarettes or more daily had a 3.7% absolute (36% relative difference) lower percentage mammographic density compared with current smokers who smoked seven cigarettes or less daily (P = 0.008). When former smokers were stratified according to time since smoking cessation, we found that women who had stopped smoking less than 24 years ago had a significantly lower mean percentage mammographic density compared with never smokers (P < 0.001). Conclusion: We found modest inverse dose–response associations between numbers of cigarettes and of pack-years smoked and both measures of mammographic density among current smokers. Former smokers who had stopped smoking less than 24 years ago also had a statistically significantly lower mean percentage mammographic density when compared with never smokers. These findings are consistent with an antiestrogenic effect of cigarette smoking on the breast tissue

Differentially expressed alternatively spliced genes in Malignant Pleural Mesothelioma identified using massively parallel transcriptome sequencing

<p>Abstract</p> <p>Background</p> <p>Analyses of Expressed Sequence Tags (ESTs) databases suggest that most human genes have multiple alternative splice variants. The alternative splicing of pre-mRNA is tightly regulated during development and in different tissue types. Changes in splicing patterns have been described in disease states. Recently, we used whole-transcriptome shotgun pryrosequencing to characterize 4 malignant pleural mesothelioma (MPM) tumors, 1 lung adenocarcinoma and 1 normal lung. We hypothesized that alternative splicing profiles might be detected in the sequencing data for the expressed genes in these samples.</p> <p>Methods</p> <p>We developed a software pipeline to map the transcriptome read sequences of the 4 MPM samples and 1 normal lung sample onto known exon junction sequences in the comprehensive AceView database of expressed sequences and to count how many reads map to each junction. 13,274,187 transcriptome reads generated by the Roche/454 sequencing platform for 5 samples were compared with 151,486 exon junctions from the AceView database. The exon junction expression index (EJEI) was calculated for each exon junction in each sample to measure the differential expression of alternative splicing events. Top ten exon junctions with the largest EJEI difference between the 4 mesothelioma and the normal lung sample were then examined for differential expression using Quantitative Real Time PCR (qRT-PCR) in the 5 sequenced samples. Two of the differentially expressed exon junctions (ACTG2.aAug05 and CDK4.aAug05) were further examined with qRT-PCR in additional 18 MPM and 18 normal lung specimens.</p> <p>Results</p> <p>We found 70,953 exon junctions covered by at least one sequence read in at least one of the 5 samples. All 10 identified most differentially expressed exon junctions were validated as present by RT-PCR, and 8 were differentially expressed exactly as predicted by the sequence analysis. The differential expression of the AceView exon junctions for the ACTG2 and CDK4 genes were also observed to be statistically significant in an additional 18 MPM and 18 normal lung samples examined using qRT-PCR. The differential expression of these two junctions was shown to successfully classify these mesothelioma and normal lung specimens with high sensitivity (89% and 78%, respectively).</p> <p>Conclusion</p> <p>Whole-transcriptome shotgun sequencing, combined with a downstream bioinformatics pipeline, provides powerful tools for the identification of differentially expressed exon junctions resulting from alternative splice variants. The alternatively spliced genes discovered in the study could serve as useful diagnostic markers as well as potential therapeutic targets for MPM.</p

Obesity and risk of pancreatic cancer among postmenopausal women: the Women's Health Initiative (United States)

A total of 138 503 women in the Women's Health Initiative in the United States were followed (for an average of 7.7 years) through 12 September 2005 to examine obesity, especially central obesity in relation to pancreatic cancer (n=251). Women in the highest quintile of waist-to-hip ratio had 70% (95% confidence interval 10–160%) excess risk of pancreatic cancer compared with women in the lowest quintile