Developing a laboratory facility to assess friction coefficients of standing samples

The numerical representation of the dynamic response of art works is the most reliable instrument to predict their seismic safety. Their adoption, however, require the knowledge of the effective mechanical properties of the artifacts and their restraint conditions. Namely, the amount of friction arising between standing art works and their supports largely affects the quality of their collapse and, therefore, the choice of the model to adopt in the analysis. In this work a facility for assessing the dynamic contact behaviour of marble sample standing on different supports has been investigated. The facility consists of a dynamic test to perform through the bidirectional shaking table at the Disaster Resilience Simulation Laboratory at the Politecnico di Torino. The friction coefficient has been found from the dynamic test by comparing the acceleration registered at the load cell, which is related to the reaction of the sample, and one measured at the shaking table surface. The obtained values of friction coefficient have been related to the velocity of the adopted input. A preliminary test on a single concrete sample has been performed to check the proposed procedure, by considering three different loading conditions: one of them is the acceleration history of a real ground motion, while the other two have a constant amplitude and a constant frequency, respectively

D2D Communications for Large-Scale Fog Platforms: Enabling Direct M2M Interactions

To many, fog computing is considered the next step beyond the current centralized cloud that will support the forthcoming Internet of Things (IoT) revolution. While IoT devices will still communicate with applications running in the cloud, localized fog clusters will appear with IoT devices communicating with application logic running on a proximate fog node. This will add proximity-based machine-to-machine (M2M) communications to standard cloud-computing traffic, and it calls for efficient mobility management for entire fog clusters and energy-efficient communication within them. In this context, long-term evolution-advanced (LTE-A) technology is expected to play a major role as a communication infrastructure that guarantees low deployment costs, native mobility support, and plug-and-play seamless configuration. We investigate the role of LTE-A in future large-scale IoT systems. In particular, we analyze how the recently standardized device-to-device (D2D) communication mode can be exploited to effectively enable direct M2M interactions within fog clusters, and we assess the expected benefits in terms of network resources and energy consumption. Moreover, we show how the fog-cluster architecture, and its localized-communication paradigm, can be leveraged to devise enhanced mobility management, building on what LTE-A already has to offer

Damage risk assessment of historical asset using laser scan and finite element approach

Most part of valuable art goods are conserved in the Museums, which are in charge of their maintenance and their exposure; some of them, however, have an outdoor location, enriching the artistic and touristic worth of the towns which host them. The art works have an intrinsic vulnerability, due to their irregular shape, slenderness, fragility and - as concerns some sculptures of historical centers - to their oldness. The outdoor artifacts, however, have further hazard sources, since they can hardly be guarded, and they are subjected to possible vandalism actions and lunatic or terrorist attacks. In this work the dynamic response of artifacts to blasts of assigned intensity are investigated on a case-study, i.e. the Fountain of Neptune, located in "Piazza della Signoria", in Florence. The Fountain of Neptune is a marble and bronze opus made by Bartolomeo Ammannati between 1560 and 1565. The main character of the Fountain, Neptune, is a marble statue 5.7 meters tall, with a weight equal to 11.5 ton. A preliminary laser scanner survey has been made to achieve the geometrical representation of the statue. The considered load condition consists of an explosion caused by 10 kg of TNT placed at 8 meters from the Fountain. The dynamic behavior of the complex under the assumed load has been represented through a numerical analysis, by considering the main statue only - since it is the slenderest element of the complex - and by assuming it as simply supported on the pedestal, without any connector. The obtained results showed the vulnerability of the statue to the assumed blast, and pointed out the role plaid by the contact assumption on its dynamic response

Anti-oxidant potential and gap junction-mediated intercellular communication as early biological markers of mercuric chloride toxicity in MDCK cell line.

In this study, the early nephrotoxic potential of mercuric chloride (HgCl(2)) has been evaluated in vitro, by exposing a renal-derived cell system, the tubular epithelial Madin-Darby canine kidney (MDCK) cell line, to the presence of increasing HgCl(2) concentrations (0.1-100 microM) for different periods of time (from 4 to 72 h). As possible biological markers of the tubular-specific toxicity of HgCl(2) in exposed-MDCK cultures we analysed: (i) critical biochemical parameters related to oxidative stress conditions and (ii) gap-junctional function (GJIC). HgCl(2) cytotoxicity was evaluated by cell-density assay. The biochemical analysis of the pro-oxidant properties of the mercuric ion (Hg(2+)) was performed by evaluating the effect of the metal salt on the antioxidant status of the MDCK cells. The cell glutathione (GSH) content and the activity of glutathione peroxidase (Gpx) and catalase (Cat), two enzymes engaged in the H(2)O(2) degradation, were quantified. HgCl(2) influence on MDCK GJIC was analysed by the microinjection/dye-transfer assay. HgCl(2)-induced morphological changes in MDCK cells were also taken into account. Our results, proving that subcytotoxic (0.1-10 microM) HgCl(2) concentrations affect either the antioxidant defences of MDCK cells or their GJIC, indicate these critical functions as suitable biological targets of early mercury-induced tubular cell injury

Effect of the administration of n-3 polyunsaturated fatty acids on circulating levels of microparticles in patients with a previous myocardial infarction

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Different Factors Affecting Human ANP Amyloid Aggregation and Their Implications in Congestive Heart Failure

Atrial Natriuretic Peptide (ANP)-containing amyloid is frequently found in the elderly heart. No data exist regarding ANP aggregation process and its link to pathologies. Our aims were: i) to experimentally prove the presumptive association of Congestive Heart Failure (CHF) and Isolated Atrial Amyloidosis (IAA); ii) to characterize ANP aggregation, thereby elucidating IAA implication in the CHF pathogenesis.A significant prevalence (85\%) of IAA was immunohistochemically proven ex vivo in biopsies from CHF patients. We investigated in vitro (using Congo Red, Thioflavin T, SDS-PAGE, transmission electron microscopy, infrared spectroscopy) ANP fibrillogenesis, starting from α-ANP as well as the ability of dimeric β-ANP to promote amyloid formation. Different conditions were adopted, including those reproducing β-ANP prevalence in CHF. Our results defined the uncommon rapidity of α-ANP self-assembly at acidic pH supporting the hypothesis that such aggregates constitute the onset of a fibrillization process subsequently proceeding at physiological pH. Interestingly, CHF-like conditions induced the production of the most stable and time-resistant ANP fibrils suggesting that CHF affected people may be prone to develop IAA.We established a link between IAA and CHF by ex vivo examination and assessed that β-ANP is, in vitro, the seed of ANP fibrils. Our results indicate that β-ANP plays a crucial role in ANP amyloid deposition under physiopathological CHF conditions. Overall, our findings indicate that early IAA-related ANP deposition may occur in CHF and suggest that these latter patients should be monitored for the development of cardiac amyloidosis

Quality of life and treatment satisfaction in adults with Type 1 diabetes: A comparison between continuous subcutaneous insulin infusion and multiple daily injections

Aims: The aim of this case-control study was to compare quality of life (QoL) and treatment satisfaction in adults with Type 1 diabetes (T1DM) treated with either continuous subcutaneous insulin infusion (CSII) or multiple daily injections (MDI). Methods: Consecutive patients aged between 18 and 55 years, and attending diabetes clinics for a routine visit, completed the Diabetes-Specific Quality-of-Life Scale (DSQOLS), the Diabetes Treatment Satisfaction Questionnaire (DTSQ) and the SF-36 Health Survey (SF-36). Case (CSII) and control subjects (MDI) were recruited in a 1 : 2 ratio. Results: Overall, 1341 individuals were enrolled by 62 diabetes clinics; 481 were cases and 860 control subjects. Cases had a longer diabetes duration and were more likely to have eye and renal complications. Age, school education, occupation and HbA1c were similar. Of control subjects, 90% followed glargine-based MDI regimens and 10% used NPH-based MDI regimens. On multivariate analysis, after adjusting for socioeconomic and clinical characteristics, scores in the following areas of the DSQOLS were higher in cases than control subjects: diet restrictions (β = 5.96; P < 0.0001), daily hassles (β = 3.57; P = 0.01) and fears about hypoglycaemia (β = 3.88; P = 0.006). Treatment with CSII was also associated with a markedly higher DTSQ score (β = 4.13; P < 0.0001) compared with MDI. Results were similar when CSII was compared separately with glargine- or NPH-based MDI regimens. Conclusions: This large, non-randomized, case-control study suggests quality of life gains deriving from greater lifestyle flexibility, less fear of hypoglycaemia, and higher treatment satisfaction, when CSII is compared with either glargine-based or NPH-based MDI regimens. © 2008 The Authors

Association of kidney disease measures with risk of renal function worsening in patients with type 1 diabetes

Background: Albuminuria has been classically considered a marker of kidney damage progression in diabetic patients and it is routinely assessed to monitor kidney function. However, the role of a mild GFR reduction on the development of stage 653 CKD has been less explored in type 1 diabetes mellitus (T1DM) patients. Aim of the present study was to evaluate the prognostic role of kidney disease measures, namely albuminuria and reduced GFR, on the development of stage 653 CKD in a large cohort of patients affected by T1DM. Methods: A total of 4284 patients affected by T1DM followed-up at 76 diabetes centers participating to the Italian Association of Clinical Diabetologists (Associazione Medici Diabetologi, AMD) initiative constitutes the study population. Urinary albumin excretion (ACR) and estimated GFR (eGFR) were retrieved and analyzed. The incidence of stage 653 CKD (eGFR < 60 mL/min/1.73 m2) or eGFR reduction > 30% from baseline was evaluated. Results: The mean estimated GFR was 98 \ub1 17 mL/min/1.73m2 and the proportion of patients with albuminuria was 15.3% (n = 654) at baseline. About 8% (n = 337) of patients developed one of the two renal endpoints during the 4-year follow-up period. Age, albuminuria (micro or macro) and baseline eGFR < 90 ml/min/m2 were independent risk factors for stage 653 CKD and renal function worsening. When compared to patients with eGFR > 90 ml/min/1.73m2 and normoalbuminuria, those with albuminuria at baseline had a 1.69 greater risk of reaching stage 3 CKD, while patients with mild eGFR reduction (i.e. eGFR between 90 and 60 mL/min/1.73 m2) show a 3.81 greater risk that rose to 8.24 for those patients with albuminuria and mild eGFR reduction at baseline. Conclusions: Albuminuria and eGFR reduction represent independent risk factors for incident stage 653 CKD in T1DM patients. The simultaneous occurrence of reduced eGFR and albuminuria have a synergistic effect on renal function worsening