442 research outputs found
A Descriptive Model of Robot Team and the Dynamic Evolution of Robot Team Cooperation
At present, the research on robot team cooperation is still in qualitative
analysis phase and lacks the description model that can quantitatively describe
the dynamical evolution of team cooperative relationships with constantly
changeable task demand in Multi-robot field. First this paper whole and static
describes organization model HWROM of robot team, then uses Markov course and
Bayesian theorem for reference, dynamical describes the team cooperative
relationships building. Finally from cooperative entity layer, ability layer
and relative layer we research team formation and cooperative mechanism, and
discuss how to optimize relative action sets during the evolution. The dynamic
evolution model of robot team and cooperative relationships between robot teams
proposed and described in this paper can not only generalize the robot team as
a whole, but also depict the dynamic evolving process quantitatively. Users can
also make the prediction of the cooperative relationship and the action of the
robot team encountering new demands based on this model. Journal web page & a
lot of robotic related papers www.ars-journal.co
Effect of Nitrogen Content on Hot Deformation Behavior and Grain Growth in Nuclear Grade 316LN Stainless Steel
316LN stainless steel with 0.08%N (08N) and 0.17%N (17N) was compressed at 1073–1473 K and 0.001–10 s−1. The hot deformation behavior was investigated using stress-strain curve analysis, processing maps, and so forth. The microstructure was analyzed through electron backscatter diffraction analysis. Under most conditions, the deformation resistance of 17N was higher than that of 08N. This difference became more pronounced at lower temperatures. The strain rate sensitivity increased with increasing temperature for types of steel. In addition, the higher the N content, the higher the strain rate sensitivity. Hot deformation activation energy increased from 487 kJ/mol to 549 kJ/mol as N concentration was increased from 0.08% to 0.17%. The critical strain for initiation of dynamic recrystallization was lowered with increasing N content. In the processing maps, both power dissipation ratio and unstable region increased with increasing N concentration. In terms of microstructure evolution, N promoted dynamic recrystallization kinetic and decreased dynamic recrystallization grain size. The grain growth rate was lower in 17N than in 08N during heat treatment. Finally, it was found that N favored twin boundary formation
CERKL regulates autophagy via the NAD-dependent deacetylase SIRT1
<p>Macroautophagy/autophagy is an important intracellular mechanism for the maintenance of cellular homeostasis. Here we show that the <i>CERKL</i> (ceramide kinase like) gene, a retinal degeneration (RD) pathogenic gene, plays a critical role in regulating autophagy by stabilizing SIRT1. <i>In vitro</i> and <i>in vivo</i>, suppressing CERKL results in impaired autophagy. SIRT1 is one of the main regulators of acetylation/deacetylation in autophagy. In CERKL-depleted retinas and cells, SIRT1 is downregulated. ATG5 and ATG7, 2 essential components of autophagy, show a higher degree of acetylation in CERKL-depleted cells. Overexpression of SIRT1 rescues autophagy in CERKL-depleted cells, whereas CERKL loses its function of regulating autophagy in SIRT1-depleted cells, and overexpression of CERKL upregulates SIRT1. Finally, we show that CERKL directly interacts with SIRT1, and may regulate its phosphorylation at Ser27 to stabilize SIRT1. These results show that CERKL is an important regulator of autophagy and it plays this role by stabilizing the deacetylase SIRT1.</p
Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
An efficient DNA extraction method was developed for peanut seed, where
only 3-5 mg cotyledonary tissue was enough for more than 50 PCR
reactions with a reaction volume of 15 \u3bcl. Both low copy number
and high copy number DNA sequences were successfully amplified.
Processing one seed sample only took about half an hour. Sampling had
no significant effects on germination and development. The DNA
extraction method makes it possible to identify transformants and
conduct molecular marker studies prior to sowing, and thus may greatly
hasten research progress
Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content
Screening of peanut seeds resulting from 0.39% sodium azide treatment
with NIRS calibration equation for bulk seed samples identified a plant
with more than 60% oleate. Oleate content in individual seeds of the
plant, as predicted by NIRS calibration equation for intact single
peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60%
oleate thus identified were further confirmed by gas chromatography.
Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild
type) and peanut seeds with elevated oleate (mutant type) revealed a
C281T transition in the coding region causing an I94T substitution in
the oleoyl-PC desaturase, which may be responsible for reduction in the
enzyme activity
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