Zikir (Maddate’) dan Implementasinya (Tarekat Khalwatiyah Samman Dusun Cempa Desa Betao Riase Kecamatan Pitu Riwa Kabbupaten Sidrap)

Hakikat atau kenyataan yang sebenarnya bahwa perjalanan spiritual yang dilakukan oleh masyarakat tarekat Khalwatiyah Samman dalam mendekatkan diri kepada Sang Pencipta mereka lebih memperbanyak mengerjakan zikir (Maddate’), sehingga dalam penerapan zikir yang dilakukan oleh masyarakat Khalwatiyah Samman memiliki cara-cara atau metode tersendiri ketika melakukan zikir (Maddate’) sebagaimana arahan atau ajaran yang diberikan oleh Mursyid. Begitupun juga dengan praktik sosialnya bahwa segala tingkah lakunya selalu di sertai dengan nuansa zikir. Penganut tarekat Khalwatiyah Samman beranggapan bahwa jalan untuk menuju kepada Sang Pencipta terdapat banyak jalan yang dapat di tempuh, akan tetapi menurut mereka, jalan tercepat untuk menuju kepada Allah swt. yaitu dengan selalu melantunkan lafal-lafal Allah swt. atau jalan zikir (maddate’) sebagaimana ajaran yang sudah ada secara turun temurun yang terdapat ditarekat Khalwatiyah Samman. Adapun pengaruh zikir (maddate’) yang dilakukan oleh penganut tarekat Khalwatiyah Samman terhadap masyarakat Samman sendiri berdampak positif, begitupun sebaliknya terhadap masyarakat Dusun Cempa yang juga mendapat pengaruh positif dalam kehidupan sosial

Effect of equivalent salt deposit density on flashover voltage of contaminated insulator energized by HVDC

In Malaysia, the demand for electric power is increasing day by day due to more consumption of power in the industrial sector. Recently, the high voltage DC transmission lines are under construction near the coastal environments for transmitting the power to the all states of Malaysia. Therefore, there is a concern about the reliability of these systems especially under adverse environmental conditions due to sea salt spray contamination. This reliability of this contaminated insulator can be improved through its performance studies. For this performance study, an analytical expression between flashover voltage and ESDD of the contaminated insulator has been proposed using Dimensional Analysis technique. The results obtained from the analytical expression are compared with the experimental results and in close agreement are foun

LITERASI DIGITAL DALAM PENGEMBANGAN MEDIA PEMBELAJARAN GURU SMKN 1 GOWA BERBASIS AI

Mayoritas Guru SMK Negeri 1 Gowa menggunakan Power Point sebagai media pembelajaran dalam menunjang proses belajar mengajar di kelas. Seiring dengan perkembangan teknologi, upgrade pengetahuan dan penguasaan teknologi menjadi suatu tuntutan kewajiban khususnya bagi guru agar tidak mengalami ketertinggalan teknologi. Media pembelajaran semakin beragam dan menyajikan berbagai fitur menarik dan kemudahan bagi pengguna. Hal tersebut memotivasi tim pengabdi untuk melakukan edukasi dan juga pelatihan IT menggunakan aplikasi Artificial Intelligence (AI) yaitu Pictory AI dan ChatGPT agar dapat mendukung pengembangan media pembelajaran Guru SMK Negeri 1 Gowa. Metode yang digunakan adalah anailisis data dengan cara melakukan wawancara langsung dengan Kepala Sekolah dan pretest bagi Guru, yang kedua metode problem solving yang dilakukan dengan presentasi, pelatihan sekaligus pendampingan, yang ketiga adalah evaluasi yaitu memberikan posttest bagi Guru. Hasil kegiatan pengabdian masyarakat adalah peningkatan pengetahuan sebesar 93,84615 % tentang google drive sebagai media penyimpanan digital dan pengembangan media pembelajaran melalui penguasaan penggunaan aplikasi AI dalam membuat video pembelajaran yang lebih menarik, cerdas dan responsif.

Complex and unexpected dynamics in simple genetic regulatory networks

Peer reviewedPublisher PD

Stable Maintenance of Multiple Plasmids in E. coli Using a Single Selective Marker

Plasmid-based genetic systems in Escherichia coli are a staple of synthetic biology. However, the use of plasmids imposes limitations on the size of synthetic gene circuits and the ease with which they can be placed into bacterial hosts. For instance, unique selective markers must be used for each plasmid to ensure their maintenance in the host. These selective markers are most often genes encoding resistance to antibiotics such as ampicillin or kanamycin. However, the simultaneous use of multiple antibiotics to retain different plasmids can place undue stress on the host and increase the cost of growth media. To address this problem, we have developed a method for stably transforming three different plasmids in E. coli using a single antibiotic selective marker. To do this, we first examined two different systems with which two plasmids may be maintained. These systems make use of either T7 RNA polymerase-specific regulation of the resistance gene or split antibiotic resistance enzymes encoded on separate plasmids. Finally, we combined the two methods to create a system with which three plasmids can be transformed and stably maintained using a single selective marker. This work shows that large-scale plasmid-based synthetic gene circuits need not be limited by the use of multiple antibiotic resistance genes

Genomic mining of prokaryotic repressors for orthogonal logic gates

Genetic circuits perform computational operations based on interactions between freely diffusing molecules within a cell. When transcription factors are combined to build a circuit, unintended interactions can disrupt its function. Here, we apply 'part mining' to build a library of 73 TetR-family repressors gleaned from prokaryotic genomes. The operators of a subset were determined using an in vitro method, and this information was used to build synthetic promoters. The promoters and repressors were screened for cross-reactions. Of these, 16 were identified that both strongly repress their cognate promoter (5- to 207-fold) and exhibit minimal interactions with other promoters. Each repressor-promoter pair was converted to a NOT gate and characterized. Used as a set of 16 NOT/NOR gates, there are >10[superscript 54] circuits that could be built by changing the pattern of input and output promoters. This represents a large set of compatible gates that can be used to construct user-defined circuits.United States. Air Force Office of Scientific Research (Award FA9550-11-C-0028)American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship (32 CFR 168a)United States. Defense Advanced Research Projects Agency. Chronical of Lineage Indicative of Origins (N66001-12-C-4016)United States. Office of Naval Research (N00014-13-1-0074)National Institutes of Health (U.S.) (GM095765)National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (SA5284-11210

Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology

Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in Escherichia coli using a novel hetero-regulation module from Pseudomonas syringae. The device comprises two co-activating genes hrpR and hrpS controlled by separate promoter inputs, and a σ54-dependent hrpL promoter driving the output. The hrpL promoter is activated only when both genes are expressed, generating digital-like AND integration behaviour. The AND gate is demonstrated to be modular by applying new regulated promoters to the inputs, and connecting the output to a NOT gate module to produce a combinatorial NAND gate. The circuits were assembled using a parts-based engineering approach of quantitative characterization, modelling, followed by construction and testing. The results show that new genetic logic devices can be engineered predictably from novel native orthogonal biological control elements using quantitatively in-context characterized parts

SYNZIP Protein Interaction Toolbox: in Vitro and in Vivo Specifications of Heterospecific Coiled-Coil Interaction Domains

The synthetic biology toolkit contains a growing number of parts for regulating transcription and translation, but very few that can be used to control protein association. Here we report characterization of 22 previously published heterospecific synthetic coiled-coil peptides called SYNZIPs. We present biophysical analysis of the oligomerization states, helix orientations, and affinities of 27 SYNZIP pairs. SYNZIP pairs were also tested for interaction in two cell-based assays. In a yeast two-hybrid screen, >85% of 253 comparable interactions were consistent with prior in vitro measurements made using coiled-coil microarrays. In a yeast-signaling assay controlled by coiled-coil mediated scaffolding, 12 SYNZIP pairs were successfully used to down-regulate the expression of a reporter gene following treatment with α-factor. Characterization of these interaction modules dramatically increases the number of available protein interaction parts for synthetic biology and should facilitate a wide range of molecular engineering applications. Summary characteristics of 27 SYNZIP peptide pairs are reported in specification sheets available in the Supporting Information and at the SYNZIP Web site [http://keatingweb.mit.edu/SYNZIP/].National Science Foundation (U.S.) (NSF award MCB 0950233)National Institutes of Health (U.S.) (grant RO1 GM55040)National Institutes of Health (U.S.) (grant PN2 EY016546)National Institutes of Health (U.S.) (grant P50 GMO81879)National Science Foundation (U.S.). Synthetic Biology Engineering Research CenterHoward Hughes Medical Institut