Folliculogenesis and follicular fluid adiponectin in cows: Its alterations and relationships with ovarian function [Folikulogeneza i adiponektin u folikularnoj tekućini krava: Promjene i odnos s funkcijom jajnika]

The objectives of the present study were to study the dynamic changes in adiponectin concentration in the growing luteal as well as the preovulatory follicles in dairy cows. In the first study, the ovaries and blood of 15 Holstein dairy cows in the luteal phase were collected from a slaughterhouse. Clear antral follicles were divided into three diameter groups (small, 3-5 mm; medium, 6-9 mm and large, ≥10 mm) and their fluid was aspirated. In the second study, the coccygeal blood and fluid of the preovulatory follicles of eight live Holstein dairy cows were aspirated transrectally, using a transrectal-guided fine-needle. Concentrations of adiponectin in the serum, and follicular fluid and progesterone in the serum were measured. Serum adiponectin concentrations in both luteal and follicular phases were higher than the follicular fluid adiponectin concentrations in all types of follicles (P0.05), and the reduction was seen in preovulatory follicles in comparison with small follicles (P = 0.001). In the luteal phase, a significant positive correlation was observed between the adiponectin concentrations in different sized follicles, and also in the serum progesterone and follicular fluid adiponectin of follicles (P<0.05). In conclusion, lower adiponectin concentrations in blood serum and preovulatory follicles in comparison to luteal growing follicles reflect the effect of ovarian stage on adiponectin alterations

Miniaturized peristaltic rotary pump for non-continuous drug dosing

Micro dosing pumps are the beating heart of infusion systems. Among many technologies to inject micro quantities of fluids, peristaltic pumps show high precision and the possibility to not alter the fluid properties. However, in real drug delivery applications, the continuous release behavior of typical peristaltic pumps is not favorable. In this paper, we investigate the intermittent performance of two prototypes of peristaltic pumps, based on four and five rollers, used to occlude the tube. The pump performances are reported for different rotation speeds and lag times between consecutive infusions. The proposed pumps showed a good volumetric precision (2.88 μL for the five rollers pump and 4.11 μL for the four rollers pump) without any dependency on rotation speed and lag time

Epigenetics of hypertension as a risk factor for the development of coronary artery disease in type 2 diabetes mellitus

Hypertension, a multifaceted cardiovascular disorder influenced by genetic, epigenetic, and environmental factors, poses a significant risk for the development of coronary artery disease (CAD) in individuals with type 2 diabetes mellitus (T2DM). Epigenetic alterations, particularly in histone modifications, DNA methylation, and microRNAs, play a pivotal role in unraveling the complex molecular underpinnings of blood pressure regulation. This review emphasizes the crucial interplay between epigenetic attributes and hypertension, shedding light on the prominence of DNA methylation, both globally and at the gene-specific level, in essential hypertension. Additionally, histone modifications, including acetylation and methylation, emerge as essential epigenetic markers linked to hypertension. Furthermore, microRNAs exert regulatory influence on blood pressure homeostasis, targeting key genes within the aldosterone and renin-angiotensin pathways. Understanding the intricate crosstalk between genetics and epigenetics in hypertension is particularly pertinent in the context of its interaction with T2DM, where hypertension serves as a notable risk factor for the development of CAD. These findings not only contribute to the comprehensive elucidation of essential hypertension but also offer promising avenues for innovative strategies in the prevention and treatment of cardiovascular complications, especially in the context of T2DM

Internal Material Flow Layers in AA6082-T6 Butt-Joints during Bobbin Friction Stir Welding

Bobbin friction stir welding with a double-sided tool configuration produces a symmetrical solid-state joint. However, control of the process parameters to achieve defect-free welds is difficult. The internal flow features of the AA6082-T6 butt-joints in bobbin friction stir welding were evaluated using a set of developed reagents and optical microscopy. The key findings are that the dark curved patterns (conventionally called 'flow-arms'), are actually oxidation layers at the advancing side, and at the retreating side are elongated grains with a high-density of accumulation of sub-grain boundaries due to dynamic recrystallization. A model of discontinuous flow within the weld is proposed, based on the microscopic observations. It is inferred that the internal flow is characterized by packets of material ('flow patches') being transported around the pin. At the retreating side they experience high localized shearing at their mutual boundaries, as evidenced in high density of sub-grain boundaries. Flow patches at the advancing side are stacked on each other and exposed to oxidization

Radiographic comparison of five different techniques for injection into the distal sesamoid bursa in cattle

Summary Numerous techniques for injection into the distal sesamoid bursa (navicular bursa) have been described, especially in equine, but there are few specific descriptions regarding this practice being done in cattle. Five different techniques were compared for injection into the distal sesamoid bursa in cattle including distal plantar approach parallel with the coronary band, proximal plantar approach, distal plantar approach parallel with the sole, abaxial approach, and distal interphalangeal joint injection. The results revealed that the numbers of needle insertion until proper placement is significantly less in the DIPJ and the DPPS techniques compared to the others (P&lt;0.05). Also, based on the times of contrast agent injection after the correct successful needle insertion, there were significant differences between DIPJ with DPPCB, PP30 and the Ab45 techniques (P&lt;0.05). According to the absence of direct communication between the distal sesamoid bursa and distal interphalangeal joint, the placement of the needle through distal plantar approach parallel with the sole was suggested

The effects of vitamin D supplementation on signaling pathway of inflammation and oxidative stress in diabetic hemodialysis: A randomized, double-blind, placebo-controlled trial

Objective: This study was carried out to determine the effects of vitamin D supplementation on signaling pathway of inflammation and oxidative stress in diabetic hemodialysis (HD) patients. Methods: This randomized double-blind placebo-controlled clinical trial was conducted among 60 diabetic HD patients. Subjects were randomly allocated into two groups to intake either vitamin D supplements at a dosage of 50,000 IU (n = 30) or placebo (n = 30) every 2 weeks for 12 weeks. Gene expression of inflammatory cytokines and biomarkers of oxidative stress were assessed in peripheral blood mononuclear cells (PBMCs) of diabetic HD patients with RT-PCR method. Results: Results of RT-PCR indicated that after the 12-week intervention, compared to the placebo, vitamin D supplementation downregulated gene expression of interleukin (IL)-1β (P = 0.02), tumor necrosis factor alpha (TNF-α) (P = 0.02) and interferon gamma (IFN-γ) (P = 0.03) in PBMCs of diabetic HD patients. Additionally, vitamin D supplementation, compared to the placebo, downregulated gene expression of transforming growth factor beta (TGF-β) (P = 0.04), protein kinase C (PKC) (P = 0.001), and mitogen-activated protein kinases 1 (MAPK1) (P = 0.02) in PBMCs of diabetic HD patients. Although not significant, vitamin D supplementation let to a reduction of nuclear factor kappa B (NF-kB) (p = 0.75) expression in PBMCs isolated from diabetic patients compared to the placebo group. There was no statistically significant change following supplementation with vitamin D on gene expression of interleukin (IL)-4, IL-6, and vascular endothelial growth factor (VEGF) in PBMCs of diabetic HD patients. Conclusions: Overall, we found that vitamin D supplementation for 12 weeks among diabetic HD patients had beneficial effects on few gene expression related to inflammation and oxidative stress. © 2018 Haddad Kashani, Seyed Hosseini, Nikzad, Soleimani, Soleimani, Tamadon, Keneshlou and Asemi

Učestalost kukca Oestrus ovis u koza u Shirazu u Južnom Iranu.

One thousand nine hundred and ninety eight heads obtained from goats slaughtered at the Fars abattoir (Shiraz, South Iran) between April 2006 and April 2007 were examined for the presence of Oestrus ovis larvae. Of the 1998 heads, 261 (13.1%) were infested with O. ovis larvae. O. ovis larvae were observed in both sexes and all age groups in each season of the year. A total of 1356 larvae were collected. The overall larval intensity for the infested goats was 5.2, with 3.2 in spring, 3.8 in summer, 4.6 in autumn and 6.8 in winter. Prevalence ranged from 6.6% in spring to 17.9% in winter. Increased infestation was observed in older animals.Ukupno je pregledano 1998 glava koza zaklanih u klaonicama Fars (Shiraz, Južni Iran) u razdoblju od travnja 2006. do tavnja 2007. Ličinke kukca Oestrus ovis bile su dokazane u 261 (13,1%) životinje bez obzira na spol i dob. Ukupno je bilo sakupljeno 1356 ličinki. Prosječna jačina invazije iznosila je 5,2 ličinke s time da su prosječno 3,2 ličinke dokazane u proljeće, 3,8 ljeti, 4,6 u jesen i 6,8 ličinki zimi. Učestalost se kretala od 6,6% u proljeće do 17,9% zimi. Veća učestalost bila je dokazana u starijih životinja

Internal Flow Behaviour and Microstructural Evolution of the Bobbin-FSW Welds: Thermomechanical Comparison between 1xxx and 3xxx Aluminium Grades

The influences of processing parameters and tool feature on the microstructure of AA1100 and AA3003 aluminium alloys were investigated using bobbin friction stir welding (BFSW). The research includes flow visualization and microstructural evolution of the weld texture using the metallographic measurement method. Results indicated that the operational parameters of the welding (e.g. feed rate, rotating speed) and the geometry of the tool can directly affect the flow patterns of the weld structure. The microscopic details revealed by the optical and electron microscope imply the dynamic recrystallization including grain refinement and precipitation mechanisms within the stirring zone of the weld region. The microscopic observations for the weld samples show a better performance of the fully-featured tool (tri-flat threaded pin and scrolled shoulders) compared to the simple tool without inscribed surface features. The fully-featured tool resulted in a more uniform thermomechanical plastic deformation within the weld structure along with the precipitation hardening and the homogeneity of the microstructure.</jats:p

Pathophysiologic mechanisms of obesity- And chronic inflammation-related genes in etiology of polycystic ovary syndrome

Objective(s): One of the common heterogeneous reproductive disorders in women of childbearing age is polycystic ovary syndrome (PCOS). It is characterized by lack of fertility due to anovulatory cycles, hyperandrogenemia, polycystic ovaries, hyperinsulinemia, and obesity. Both reproductive anomalies and metabolic disorders are involved in PCOS pathology. Although the role of increased levels of androgens in initiation of PCOS is almost proven, mechanisms of PCOS pathophysiology are not clear. Here we discuss roles of altered metabolic conditions, obesity, and chronic inflammation in PCOS pathophysiology. Materials and Methods:: In this review, we attempted to identify genes related to obesity and chronic inflammation aspects of PCOS and their physiological functions to explain the pathways that are regulated by these genes and can be a prominent function in PCOS predisposition. For this purpose, published articles and reviews dealing with genetic evaluation of PCOS in women in peer-reviewed journals in PubMed and Google Scholar databases were included in this review. Results: Obesity and chronic inflammation are not prominent diagnostic features of PCOS, but they play an important role in exacerbating metabolic and hyperandrogenic states. ADIPOQ, FTO TGFß, and DENND1A as the main obesity- and chronic inflammation-related genes have roles in PCOS pathophysiology. Conclusion: It seems that genes related to obesity pathology in genomic research association, are related to metabolic aspects and body mass index in PCOS patients. Genomes have roles in chronic inflammation, followed by obesity, in the pathogenesis of PCO

3D Printing of Small-Scale Soft Robots with Programmable Magnetization

status: publishe