Additional file 4: Figure S3. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Masson’s trichrome-stained histological sections of the gastrocnemius and quadriceps from 8- to 18-month-old mice of the indicated genotypes. Scale bar = 50 μm. The number of mice is 6–8 for each group

Additional file 3: Figure S2. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

H&E-stained histological sections of the gastrocnemius and quadriceps from 8-month-old mice of the indicated genotypes. Scale bar = 50 μm. The number of mice is 6–8 for each group

Additional file 9: Table S4. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Gene list. List of genes that are upregulated in the KO skeletal muscles

Additional file 2: Figure S1. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Test of anti-Ano5 antibodies by Western blotting. a The anti-Ano5 antibody (sc-169626) from Santa Cruz Biotechnology did not detect any positive signal in human Ano5-expressing cell lysate, which was correctly detected by the anti-GFP antibody at the predicted size. b The anti-Ano5 antibody (AP8580B) from Abgent produced a prominent band at about 100 kDa in both negative and positive samples; however, this band is not Ano5 because the Ano5-YFP fusion protein should be around 135 kDa as correctly detected by the anti-GFP antibody. The arrows point to the correct Ano5-YFP fusion protein. These experiments were repeated at least three times

Additional file 6: Figure S5. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Representative images of Masson’s trichrome-stained histological sections of WT and Ano5 KO hearts after PBS or ISO treatments for 2 weeks. Scale bar = 50 μm. The number of mice (18 months of age) is 6–8 for each group

Additional file 8: Table S3. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Gene list. List of genes that are downregulated in the KO skeletal muscles

Additional file 7: Table S2. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

Echocardiographic measurements. Echocardiographic measurements in WT and Ano5 KO mice after isoproterenol injections