Test of anti-Ano5 antibodies by Western blotting. a The anti-Ano5 antibody (sc-169626) from Santa Cruz Biotechnology did not detect any positive signal in human Ano5-expressing cell lysate, which was correctly detected by the anti-GFP antibody at the predicted size. b The anti-Ano5 antibody (AP8580B) from Abgent produced a prominent band at about 100Â kDa in both negative and positive samples; however, this band is not Ano5 because the Ano5-YFP fusion protein should be around 135Â kDa as correctly detected by the anti-GFP antibody. The arrows point to the correct Ano5-YFP fusion protein. These experiments were repeated at least three times
