Anisotropic Energy Distribution in Three-Dimensional Vibrofluidized Granular Systems

We examine the energy distribution in a three-dimensional model granular system contained in an open cylinder under the influence of gravity. Energy is supplied to the system by a vibrating base. We introduce spatially resolved, partial particle-particle ``dissipations'' for directions parallel and perpendicular to the energy input, respectively. Energy balances show that the total (integrated) ``dissipation'' is less than zero in the parallel direction while greater than zero in the perpendicular directions. The energy supplied to the perpendicular directions is dissipated by particle-wall collisions. We further define a fractional energy transfer, which in the steady state represents the fraction of the power supplied by the vibrating base that is dissipated at the wall. We examine the dependence of the fractional energy transfer on the number of particles, the velocity of the vibrating base, the particle-particle restitution coefficient, and the particle-wall restitution coefficient. We also explore the influence of the system parameters on the spatially dependent partial dissipations.Comment: 10 pages, 10 figures, RevTeX forma

Public domain small-area cancer incidence data for New York State, 2005-2009

There has long been a demand for cancer incidence data at a fine geographic resolution for use in etiologic hypothesis generation and testing, methodological evaluation, and teaching. In this paper we describe a public domain data set containing data for 23 anatomic sites of cancer diagnosed in New York State between 2005 and 2009 at the level of the census block group. The data set includes 524,503 tumors distributed across 13,823 block groups with an average population of about 1,400. In addition, the data have been linked with race and ethnicity and with socioeconomic indicators such as income, educational attainment, and language proficiency. We demonstrate the application of the data set by confirming two well-established relationships: that between breast cancer and median household income, and that between stomach cancer and Asian race. We foresee that this data set will serve as the basis for a wide range of spatial analyses and serve as a benchmark data set for evaluating spatial methods in the future

Exact solution of a one-dimensional Boltzmann equation for a granular tracer particle

We consider a one-dimensional system consisting of a granular tracer particle of mass $M$ in a bath of thermalized particles each of mass $m$. When the mass ratio, $M/m$, is equal to the coefficient of restitution, $\alpha$, the system maps to a a one-dimensional elastic gas. In this case, Boltzmann equation can be solved exactly. We also obtain expressions for the velocity autocorrelation function and the diffusion coefficient. Numerical simulations of the Boltzmann equation are performed for $M/m\neq \alpha$ where no analytical solution is available. It appears that the dynamical features remain qualitatively similar to those found in the exactly solvable case.Comment: 17 pages, 3 figures, Accepted in Physica

Evaluation of Dynamic Cell Processes and Behavior Using Video Bioinformatics Tools

Just as body language can reveal a person’s state of well-being, dynamic changes in cell behavior and morphology can be used to monitor processes in cultured cells. This chapter discusses how CL-Quant software, a commercially available video bioinformatics tool, can be used to extract quantitative data on: (1) growth/proliferation, (2) cell and colony migration, (3) reactive oxygen species (ROS) production, and (4) neural differentiation. Protocols created using CL-Quant were used to analyze both single cells and colonies. Time-lapse experiments in which different cell types were subjected to various chemical exposures were done using Nikon BioStations. Proliferation rate was measured in human embryonic stem cell colonies by quantifying colony area (pixels) and in single cells by measuring confluency (pixels). Colony and single cell migration were studied by measuring total displacement (distance between the starting and ending points) and total distance traveled by the colonies/cells. To quantify ROS production, cells were pre-loaded with MitoSOX Red™, a mitochondrial ROS (superoxide) indicator, treated with various chemicals, then total intensity of the red fluorescence was measured in each frame. Lastly, neural stem cells were incubated in differentiation medium for 12 days, and time lapse images were collected daily. Differentiation of neural stem cells was quantified using a protocol that detects young neurons. CLQuant software can be used to evaluate biological processes in living cells, and the protocols developed in this project can be applied to basic research and toxicological studies, or to monitor quality control in culture facilities

Thermalization of an anisotropic granular particle

We investigate the dynamics of a needle in a two-dimensional bath composed of thermalized point particles. Collisions between the needle and points are inelastic and characterized by a normal restitution coefficient $\alpha<1$. By using the Enskog-Boltzmann equation, we obtain analytical expressions for the translational and rotational granular temperatures of the needle and show that these are, in general, different from the bath temperature. The translational temperature always exceeds the rotational one, though the difference decreases with increasing moment of inertia. The predictions of the theory are in very good agreement with numerical simulations of the model.Comment: 7 pages, 6 Figures, submitted to PRE. Revised version (Fig1, Fig5 and Fig6 corrected + minor typos

Analysis of Granular Flow in a Pebble-Bed Nuclear Reactor

Pebble-bed nuclear reactor technology, which is currently being revived around the world, raises fundamental questions about dense granular flow in silos. A typical reactor core is composed of graphite fuel pebbles, which drain very slowly in a continuous refueling process. Pebble flow is poorly understood and not easily accessible to experiments, and yet it has a major impact on reactor physics. To address this problem, we perform full-scale, discrete-element simulations in realistic geometries, with up to 440,000 frictional, viscoelastic 6cm-diameter spheres draining in a cylindrical vessel of diameter 3.5m and height 10m with bottom funnels angled at 30 degrees or 60 degrees. We also simulate a bidisperse core with a dynamic central column of smaller graphite moderator pebbles and show that little mixing occurs down to a 1:2 diameter ratio. We analyze the mean velocity, diffusion and mixing, local ordering and porosity (from Voronoi volumes), the residence-time distribution, and the effects of wall friction and discuss implications for reactor design and the basic physics of granular flow.Comment: 18 pages, 21 figure

Field induced stationary state for an accelerated tracer in a bath

Our interest goes to the behavior of a tracer particle, accelerated by a constant and uniform external field, when the energy injected by the field is redistributed through collision to a bath of unaccelerated particles. A non equilibrium steady state is thereby reached. Solutions of a generalized Boltzmann-Lorentz equation are analyzed analytically, in a versatile framework that embeds the majority of tracer-bath interactions discussed in the literature. These results --mostly derived for a one dimensional system-- are successfully confronted to those of three independent numerical simulation methods: a direct iterative solution, Gillespie algorithm, and the Direct Simulation Monte Carlo technique. We work out the diffusion properties as well as the velocity tails: large v, and either large -v, or v in the vicinity of its lower cutoff whenever the velocity distribution is bounded from below. Particular emphasis is put on the cold bath limit, with scatterers at rest, which plays a special role in our model.Comment: 20 pages, 6 figures v3:minor corrections in sec.III and added reference

Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis.

Highlights: Feline antibodies against cryptic Aspergillus spp. cross react with an aspergillin containing A. fumigatus antigens. • Brachycephalic cats are prone to upper respiratory tract aspergillosis (URTA). • The agar gel immunodiffusion (AGID) assay has low sensitivity for diagnosis of URTA. • IgG ELISA has high sensitivity and specificity for diagnosis of URTA. • This study provides evidence that cats with URTA are systemically immunocompetent. Abstract Feline upper respiratory tract aspergillosis (URTA) is an emerging infectious disease. The aims of this study were: (1) to assess the diagnostic value of detection of Aspergillus-specific antibodies using an agar gel double immunodiffusion (AGID) assay and an indirect immunoglobulin G (IgG) ELISA; and (2) to determine if an aspergillin derived from mycelia of Aspergillus fumigatus, Aspergillus niger and Aspergillus flavus can be used to detect serum antibodies against cryptic Aspergillus spp. in Aspergillus section Fumigati. Sera from cats with URTA (group 1: n = 21) and two control groups (group 2: cats with other upper respiratory tract diseases, n = 25; group 3: healthy cats and cats with non-respiratory, non-fungal illness, n = 84) were tested. Isolates from cats with URTA comprised A. fumigatus (n = 5), A. flavus (n = 1) and four cryptic species: Aspergillus felis (n = 12), Aspergillus thermomutatus (Neosartorya pseudofischeri, n = 1), Aspergillus lentulus (n = 1) and Aspergillus udagawae (n = 1). Brachycephalic purebred cats were significantly more likely to develop URTA than other breeds (P = 0.013). The sensitivity (Se) of the AGID was 43% and the specificity (Sp) was 100%. At a cut-off value of 6 ELISA units/mL, the Se of the IgG ELISA was 95.2% and the Sp was 92% and 92.9% for groups 2 and 3 cats, respectively. Aspergillus-specific antibodies against all four cryptic species were detected in one or both assays. Assay Se was not associated with species identity. Detection of Aspergillus-specific antibodies by IgG ELISA has high Se and Sp for diagnosis of feline URTA. Keywords: Aspergillosis; Aspergillus spp; Sino-nasal; Sino-orbital; Felinefunded by an Australian Companion Animal Health Foundation grant (015/201

Enhanced viability of corneal epithelial cells for efficient transport/storage using a structurally-modified calcium alginate hydrogel

Aims: Therapeutic limbal epithelial stem cells could be managed more efficiently if clinically validated batches were transported for ‘on-demand’ use. Materials & methods: In this study, corneal epithelial cell viability in calcium alginate hydrogels was examined under cell culture, ambient and chilled conditions for up to 7 days. Results: Cell viability improved as gel internal pore size increased, and was further enhanced with modification of the gel from a mass to a thin disc. Ambient storage conditions were optimal for supporting cell viability in gel discs. Cell viability in gel discs was significantly enhanced with increases in pore size mediated by hydroxyethyl cellulose. Conclusion: Our novel methodology of controlling alginate gel shape and pore size together provides a more practical and economical alternative to established corneal tissue/cell storage methods