Flavor-Dependence and Higher Orders of Gauge-Independent Solutions in Strong Coupling Gauge Theory

The fermion flavor $N_f$ dependence of non-perturbative solutions in the strong coupling phase of the gauge theory is reexamined based on the interrelation between the inversion method and the Schwinger-Dyson equation approach. Especially we point out that the apparent discrepancy on the value of the critical coupling in QED will be resolved by taking into account the higher order corrections which inevitably lead to the flavor-dependence. In the quenched QED, we conclude that the gauge-independent critical point $\alpha_c=2\pi/3$ obtained by the inversion method to the lowest order will be reduced to the result $\alpha_c=\pi/3$ of the Schwinger-Dyson equation in the infinite order limit, but its convergence is quite slow. This is shown by adding the chiral-invariant four-fermion interaction.Comment: CHIBA-EP-72, 13 pages (including 1 Table), LaTex fil

Sortase A-assisted metabolic enzyme ligation in Escherichia coli

We demonstrated the metabolic enzyme ligation by sortase A-mediated ligation (sortagging) for the redirection of metabolic flux thorough metabolic channeling. Staphylococcal sortase A (SrtA) is utilized for the ligation of metabolic enzymes. SrtA is transpeptidase, which recognizes Leu-Pro-Xaa-Thr-Gly sequences (LP tag) and cleaves between Thr and Gly, and subsequently links amino group of oligoglycine (G tag) thorough a native peptide bond. Sortagging enables to conjugate protein with other molecules in a site-specific manner. Minimal modifications of protein with short peptide tags; LP tag and G tag are only required for site-specific ligation. Hence, sortagging has been utilized for preparing a variety of bioconjugation not only in vitro but also in vivo.1 In current study, we hypothesize that SrtA-mediated metabolic enzyme ligation in cytoplasm of Escherichia coli facilitates processing metabolic intermediate, and redirects metabolic fluxes to desired pathway. As proof of concept, we constructed acetate producing E. coli with engineered endogenous metabolic pathway, which redirect central metabolic fluxes to acetate producing flux by the induction of chemical additives (Figure 1). The expression of SrtA was controlled by Lac operating promoter, metabolic channeling was videlicet occurred by the addition of IPTG. Acetyl-CoA was chosen as the intermediate model because acetyl-CoA is one of the most important central metabolic intermediates, which is converted to alcohols, fatty acids, and mevalonate derivatives. In this study, we tested covalent linking of pyruvate-formate lyase and phosphate acetyltransferase by sortase A-mediated ligation and evaluated the production of acetate. The time point of addition of IPTG was not critical for facilitating metabolic enzyme ligation, and acetate production increased upon expression of sortase A. These results show that sortase A-mediated enzyme ligation enhances an acetate-producing flux in E. coli. We have validated that sortase A-mediated enzyme ligation offers a metabolic channeling approach to redirect a central flux to a desired flux.2 Please click Additional Files below to see the full abstract

H_2 Dissociative Adsorption at the Armchair Edges of Graphite

We investigate and discuss how hydrogen behaves at the edges of a graphite sheet, in particular the armchair edge. Our density functional theory-based calculations results show that, in contrast to the zigzag edge [cf., e-J. Surf. Sci. Nanotech. 2 (2004) 77], regardless of orientation, there is an activation barrier hindering H_2 dissociation at the armchair edges. And once they do get dissociatively adsorbed at the armchair edges, we find that it would be extremely hard to desorb the H from their adsorption sites at the armchair edges. Furthermore, we also found that, consistent with our earlier conclusions [cf., J. Phys. Soc. Jpn. 72 (2003) 1867], it is unlikely that we would find a whole H_2 in between plain graphite sheets.Comment: 4 pages, 5 figures, preprin

Dynamical Breakdown of Chirality and Parity in (2+1)-dimensional QED

In the (2+1)-dimensional QED with and without the Chern-Simons term, we find the non-local gauge in which there is no wavefunction renormalization for the fermion in the framework of the Schwinger-Dyson equation. By solving the Schwinger-Dyson equation in the non-local gauge, we find a finite critical value $N_f^c$ for the number of flavors $N_f$ of four-component Dirac fermions, above which the chiral symmetry restores irrespective of presence or absence of the Chern-Simons term. In the same framework, we study the possibility of dynamical breakdown of the parity. It is shown that the parity is not dynamically broken. We discuss this reason from the viewpoint of the Schwinger-Dyson equation.Comment: 22 pages, LaTeX, to be published in Nucl. Phys.

Different Responses to 5-fluoraouracil in Mutagenicity and Gene Expression between Two Human Lymphoblastoid Cell Lines with or without TP53 Mutation

Human lymphoblastoid TK6 and WTK-1 cells are widely used to detect mutagens in vitro. TK6 cells have wild-type TP53 alleles, while WTK-1 cells have one allele of mutated TP53. Both cells were treated with 5-fluorouracil (5-FU), and gene mutation assay and micronucleus assay were performed to clarify the differential response related to the TP53 gene status. The effects of 5-FU on gene expression were assessed by microarray and quantitative RT-PCR analyses. In WTK-1 cells, 5-FU increased the frequency of cells with micronucleus and mutation. In TK6 cells, frequency of cells with micronucleus was increased but the mutation frequency was not. The cytotoxicity induced by 5-FU was more prominent in TK6 cells than in WTK-1 cells. Analysis of gene expression showed that the genes involved in the TP53 pathway were up-regulated in TK6 cells but not in WTK-1 cells. The differential responses to 5-FU between these cell lines appeared to be due to the difference in the TP53 gene status, thus providing a molecular basis for the bioassays using these cell lines in the toxicology field. Our results indicate that the clinical efficacy of 5-FU chemotherapy may depend on the TP53 genotype

Seronegative Oligoarthritis Preceding Psoriasis by 9.5 Years

We report a case of psoriatic arthritis where oligoarthritis preceded the skin lesions. A 57-year-old man complained of left third-finger pain. Laboratory examinations were negative for anti-cyclic citrullinated peptide antibodies and rheumatoid factor; he was treated for suspected rheumatoid arthritis. Six years later, X-ray revealed enthesitis of his fingers and wrist joint. At 9.5 years after the initial visit, skin lesions appeared in the left auricular region and buttock and dermatopathology findings indicated psoriasis vulgaris. The final diagnosis was psoriatic arthritis. In cases of seronegative oligoarthritis, psoriatic arthritis must be considered because some patients demonstrate osteoarticular lesions preceding skin lesions

Next‐generation sequencing in two cases of de novo acute basophilic leukaemia

Acute basophilic leukaemia (ABL) is a rare subtype of acute myeloid leukaemia (AML); therefore, few data are available about its biology. Herein, we analysed two ABL patients using flow cytometry and next-generation sequencing (NGS). Two cell populations were detected by flow cytometry in both patients. In Case no. 1, blasts (CD34⁺, CD203c⁻, CD117⁺, CD123dim⁺) and basophils (CD34⁻, CD203c⁺, CD117±, CD123⁺) were identified, both of which were found by NGS to harbour the 17p deletion and have loss of heterozygosity of TP53. In Case no. 2, blasts (CD33⁺, CD34⁺, CD123⁻) and basophils (CD33⁺, CD34⁺, CD123⁺) were identified. NGS detected NPM1 mutations in either blasts or basophils, and TET2 in both. These data suggest an overlap of the mutational landscape of ABL and AML, including TP53 and TET2 mutations. Moreover, additional mutations or epigenetic factors may contribute for the differentiation into basophilic blasts

Numerical analysis of active magnetic regenerators for hydrogen magnetic refrigeration between 20 and 77 K

金沢大学理工研究域数物科学系A model active magnetic regenerator refrigerator (AMR) with the Brayton-like operation cycle was analyzed by numerical cycle simulation in the temperature range between 20 and 77 K. In order to study the performance using magnetic material with various transition temperatures Tc, entropy of magnetic material with second order phase transition was calculated using mean field theory and Debye approximation. The cooling performance is shown to be high when the heat exhaust temperature is close to the transition temperature. It is shown that the optimized operation condition depends on both Tc and operation temperatures. Multi-layered AMR beds were shown to improve the performance of AMR. Multi-stage AMR was also discussed. © 2010