Identification of Second Messenger Mediating Signal Transduction in the Olfactory Receptor Cell

One of the biggest controversial issues in the research of olfaction has been the mechanism underlying response generation to odorants that have been shown to fail to produce cAMP when tested by biochemical assays with olfactory ciliary preparations. Such observations are actually the original source proposing a possibility for the presence of multiple and parallel transduction pathways. In this study the activity of transduction channels in the olfactory cilia was recorded in cells that retained their abilities of responding to odorants that have been reported to produce InsP3 (instead of producing cAMP, and therefore tentatively termed “InsP3 odorants”). At the same time, the cytoplasmic cNMP concentration ([cNMP]i) was manipulated through the photolysis of caged compounds to examine their real-time interactions with odorant responses. Properties of responses induced by both InsP3 odorants and cytoplasmic cNMP resembled each other in their unique characteristics. Reversal potentials of currents were 2 mV for InsP3 odorant responses and 3 mV for responses induced by cNMP. Current and voltage (I-V) relations showed slight outward rectification. Both responses showed voltage-dependent adaptation when examined with double pulse protocols. When brief pulses of the InsP3 odorant and cytoplasmic cNMP were applied alternatively, responses expressed cross-adaptation with each other. Furthermore, both responses were additive in a manner as predicted quantitatively by the theory that signal transduction is mediated by the increase in cytoplasmic cAMP. With InsP3 odorants, actually, remarkable responses could be detected in a small fraction of cells (∼2%), explaining the observation for a small production of cAMP in ciliary preparations obtained from the entire epithelium. The data will provide evidence showing that olfactory response generation and adaptation are regulated by a uniform mechanism for a wide variety of odorants

A model for the infrared dust emission from forming galaxies

In the early epoch of galaxy evolution, dust is only supplied by supernovae (SNe). With the aid of a new physical model of dust production by SNe developed by Nozawa et al. (2003) (N03), we constructed a model of dust emission from forming galaxies on the basis of the theoretical framework of Takeuchi et al. (2003) (T03). N03 showed that the produced dust species depends strongly on the mixing within SNe. We treated both unmixed and mixed cases and calculated the infrared (IR) spectral energy distribution (SED) of forming galaxies for both cases. Our model SED is less luminous than the SED of T03 model by a factor of 2-3. The difference is due to our improved treatment of UV photon absorption cross section, as well as different grain size and species newly adopted in this work. The SED for the unmixed case is found to have an enhanced near to mid-IR (N-MIR) continuum radiation in its early phase of the evolution (age < 10^{7.25} yr) compared with that for the mixed case. The strong N--MIR continuum is due to the emission from Si grains, which only exist in the species of the unmixed dust production. We also calculated the IR extinction curves for forming galaxies. Then we calculated the SED of a local starbursting dwarf galaxy SBS 0335-052. Our present model SED naturally reproduced the strong N--MIR continuum and the lack of cold FIR emission of SBS 0335-052. We found that only the SED of unmixed case can reproduce the NIR continuum of this galaxy. We then made a prediction for the SED of another typical star-forming dwarf, I Zw 18. We also presented the evolution of the SED of LBGs. Finally, we discussed the possibility of observing forming galaxies at z > 5.Comment: MNRAS, in press. 18 pages, 15 figures. Abstract abridge

Alcohol Consumption or Excessive Use of Psychotropic Medication Prior to Suicidal Self-injury in Patients with Adjustment Disorder, Depression, and Schizophrenia: A Cross-sectional Study

The use of alcohol or drug(s) prior to self-injury is a possible inducing factor for suicidal self-injuries among patients with adjustment disorder. We analyzed the cases of 175 individuals who were admitted to the intensive care unit (ICU) of Tokyo Medical and Dental University Medical Hospital for suicidal self-injury to determine whether alcohol consumption or an excessive use of prescribed psychotropic medications prior to self-injury is more common in patients with adjustment disorder. During a 7-year period (July 2006 to June 2013) following their deliberate self-injuries, 971 patients were admitted to the ICU. Our study sample (n=175) was restricted to patients with adjustment disorder (n=48), major depressive disorder (n=90), or schizophrenia (n=37). The outcome variable was alcohol consumption or excessive use of medications prior to suicidal self-injury. A logistic regression analysis revealed that the patients with adjustment disorder more commonly showed alcohol consumption or excessive medication use prior to their suicidal self-injury compared to those with schizophrenia (odds ratio: 8.10; 95%CI: 2.97-24.60). To inhibit suicidal self-injury among patients with adjustment disorder, it is important to continue efforts to provide psychoeducation about alcohol use and to instruct the patients to take their prescribed medication(s) only as directed by their physician