Molecular versus morphological markers to describe variability in sugar cane (Saccharum officinarum) for germplasm management and conservation

Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding.Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding

Molecular versus morphological markers to describe variability in sugar cane (Saccharum officinarum) for germplasm management and conservation

Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding.Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding

Molecular versus morphological markers to describe variability in sugar cane (Saccharum officinarum) for germplasm management and conservation

Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding.Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes. Highlights Although both morphologic and molecular markers resulted discriminative enough to differentiate among sugarcane accessions, it was not possible to correlate associations of markers with the origin of materials. The large number of pair-wise similarity coefficients with intermediate values determined a rather small number of nodes in clustering, which, reflects the near genetic origin of most of the materials investigated. Diversity detected for morphological descriptors is expected since they are not usually subjected to selection inbreeding

Molecular versus morphological markers to describe variability in sugar cane (Saccharum officinarum) for germplasm management and conservation = Marcadores moleculares y morfológicos para la descripción de variabilidad en caña de azúcar (Saccharum officinarum) con fines de manejo y conservación de germoplasma

Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markers A good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes.La caña de azúcar es uno de los cultivos industriales más importantes de regiones tropicales y subtropicales. El INTA (Argentina) administra un Banco de Germoplasma de caña de azúcar y lleva a cabo un programa de mejora. El presente trabajo fue diseñado para estimar la variabilidad fenotípica y genética entre 65 accesiones de caña de azúcar seleccionadas del INTA. Se aplicaron métodos de clasificación y ordenamiento en el análisis de datos morfológicos y de SSR. EL Análisis de Procrustes Generalizado permitió evaluar la correlación entre las relaciones establecidas a partir de ambos tipos de marca-dores. Un buen ajuste entre los dendrogramas y las matrices de similitud fue soportado por un alto coeficiente de correlación cofenética (r=0,82, p<0,0001; r=0,73, p<0,0001; r=0,82, p<0,0001 para datos cuantitativos, cualitativos y moleculares respectivamente). La presencia de una estructura poblacional fue reconocida cuando se consideraron los diferentes tipos de datos. El Procrustes permitió detectar aquellas accesiones que serían responsables de la baja correlación detectada entre configuraciones individuales (73%). Tanto los marcadores morfológicos como los moleculares resultaron lo suficientemente discriminativos para diferenciar accesiones. No obstante, no fue posible correlacionar las asociaciones establecidas por los marcadores con el origen de los materiales. Las distancias fenotípicas y genéticas basadas en información morfológica y molecular será de utilidad para asistir en la conservación y organización de los materiales de la colección y elegir combinaciones parentales con propósito de mejora.EEA FamailláFil: Pocovi, Mariana Ines. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Collavino, Norma Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Gutiérrez, Ángela. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Taboada, Gisel María. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Castillo, Verónica. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Delgado, Romina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; ArgentinaFil: Mariotti Martinez, Jorge Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Laboratorio de Marcadores Moleculares; Argentin

Marcadores moleculares y morfológicos para la descripción de variabilidad en caña de azúcar (Saccharum officiarum) con fines de manejo y conservación de germoplasma

La caña de azúcar es uno de los cultivos industriales más importantes de regiones tropicales y subtropicales. El INTA (Argentina) administra un Banco de Germoplasma de caña de azúcar y lleva a cabo un programa de mejora. El presente trabajo fue diseñado para estimar la variabilidad fenotípica y genética entre 65 accesiones de caña de azúcar seleccionadas del INTA. Se aplicaron métodos de clasificación y ordenamiento en el análisis de datos morfológicos y de SSR. EL Análisis de Procrustes Generalizado permitió evaluar la correlación entre las relaciones establecidas a partir de ambos tipos de marcadores. Un buen ajuste entre los dendrogramas y las matrices de similitud fue soportado por un alto coeficiente de correlación cofenética (r=0,82, p<0,0001; r=0,73, p<0,0001; r=0,82, p<0,0001 para datos cuantitativos, cualitativos y moleculares respectivamente). La presencia de una estructura poblacional fue reconocida cuando se consideraron los diferentes tipos de datos. El Procrustes permitió detectar aquellas accesiones que serían responsables de la baja correlación detectada entre configuraciones individuales (73%). Tanto los marcadores morfológicos como los moleculares resultaron lo suficientemente discriminativos para diferenciar accesiones. No obstante, no fue posible correlacionar las asociaciones establecidas por los marcadores con el origen de los materiales. Las distancias fenotípicas y genéticas basadas en información morfológica y molecular será de utilidad para asistir en la conservación y organización de los materiales de la colección y elegir combinaciones parentales con propósito de mejora.Sugarcane is one of the most important industrial crops in tropical and subtropical regions. INTA (Argentina) administrates a Sugarcane Germplasm Bank and carries out a breeding program. The current study was designed to assess the phenotypic and genetic diversity among 65 sugarcane accessions selected from the INTA. Clustering and ordination methods based on quantitative and qualitative morphological traits and SSR data were applied. Generalized Procrustes Analysis allowed evaluating the correlation between relationships established with both markersA good of fit between dendrograms and similarity matrices were revealed by high cophenetic coefficients (r=0.82, p<0.0001; r=0.73, p<0.0001; r=0.82, p<0.0001 for phenotypic quantitative, phenotypic qualitative and molecular data respectively). The presence of different reliable population structure was observed when considering different data sources. Procrustes allowed finding those accessions that should have been responsible for the low correlation found between the individual configurations (73%). Both morphologic and molecular markers resulted discriminative enough to differentiate among accessions. It was not possible, however, to correlate associations of markers with the origin of materials. Phenotypic and genetic distances based on morphology and molecular information serves to assist conservation and organization of collection of materials, and the choice of parent combinations for breeding purposes.Fil: Pocovi, Mariana Inés. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Collavino, Norma Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Gutiérrez, Angela Verónica. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Taboada, Gisel María. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Castillo, Veronica. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Delgado, Romina Paola. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Mariotti, Jorge. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentin

Development of novel ssr molecular markers using a next-generation sequencing approach (Ddradseq) in stetsonia coryne (cactaceae)

The Cactaceae family is native to the American continent with several centers of diversity. In South America, one of these centers is the Central Andes and many species are considered to be threatened or vulnerable according to the International Union for Conservation of Nature (IUCN). Stetsonia Coryne is an emblematic giant columnar of the Chaco phytogeographic province. It has an extensive geographical distribution in many countries of the continent. However, to date there are no specific molecular markers for this species, neither reports of population genetic variability studies, such as for many cactus species. The lack of information is fundamentally due to the lack of molecular markers that allow these studies. In this work, by applying a Genotyping by Sequencing (GBS) technique, we developed polymorphic SSR markers for the Stetsonia coryne and evaluated their transferability to phylogenetically close species, in order to account for a robust panel of molecular markers for multispecies-studies within Cactaceae.Fil: Gutiérrez, Angela Verónica. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Agronomía. Laboratorio de Investigaciones Botánicas; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Gerencia de Gestion Estrategica de Procesos Complementarios.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Filippi, Carla Valeria. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Gerencia de Gestion Estrategica de Procesos Complementarios.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Gerencia de Gestion Estrategica de Procesos Complementarios.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Gerencia de Gestion Estrategica de Procesos Complementarios.; ArgentinaFil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Gerencia de Gestion Estrategica de Procesos Complementarios.; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Taboada, Gisel María. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Agronomía. Laboratorio de Investigaciones Botánicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Ortega Baes, Francisco Pablo. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Escuela de Agronomía. Laboratorio de Investigaciones Botánicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentin

Screening common bean germplasm for resistance to genetically diverse Sclerotinia sclerotiorum isolates from Argentina

White mold caused by Sclerotinia sclerotiorum (Lib.) de Bary is a devastating disease that affects the common bean (Phaseolus vulgaris. L) crop worldwide. In Argentina, white mold has been detected in all bean production areas, reaching seed yield and quality losses up to 100% on susceptible common bean cultivars under favorable weather conditions. The aim of this study was to screen the physiological resistance of 20 common bean accessions to five genetically distinct isolates of S. sclerotiorum collected from the main common bean growing area of Argentina, using the greenhouse straw test. The white mold reaction was scored at 7, 14, and 21 days post-inoculation using a 1 (no disease symptoms) to 9 (severely diseased or dead plants) scale and the area under the disease progress curve (AUDPC) was determined. Highly significant differences (p < 0.001) were observed between isolates, accessions and genotype x isolate interaction at the three evaluations dates. All cultivars and lines were susceptible at the end of the assessment, except line A 195 which was resistant to white mold against the five isolates tested and was significantly different from all accessions. This work represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.Fil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Casalderrey, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; ArgentinaFil: Maggio, María Elisa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; ArgentinaFil: Chocobar, Mario Osvaldo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; ArgentinaFil: Spedaletti, Yamila Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Aparicio González, Mónica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Vizgarra, Oscar Vicéforo. Gobierno de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial Obispo Colombres; ArgentinaFil: Galván, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentin

Molecular identification and pathogenicity of Rhizoctonia spp. recovered from seed and soil samples of the main bean growing area of Argentina

The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species.Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Mercado Cárdenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Taboada, Gisel María. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Aban, Carla Luciana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Aparicio González, Mónica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Rodriguero, Marcela Silvia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Laboratorio de Ecología Funcional; ArgentinaFil: Vizgarra, Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial "Obispo Colombres" (p). Instituto de Tecnología Agroindustrial del Noroeste Argentino; ArgentinaFil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Galindez, Guadalupe. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Galván, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentin

Development and validation of a new artificial intelligence tool (GeneClin) for the clinical diagnosis of genetic diseases

Introduction: Advances in the field of Artificial Intelligence (AI) and Machine Learning (ML) have considerable potential to improve the diagnosis and management of rare genetic diseases, due to the human inability to memorize information on a multitude of these diseases, which AI tools could store, analyze and integrate. Objective: to develop and validate a new AI tool for the clinical diagnosis of genetic diseases. Methods: A prospective, cross-sectional, analytical, observational study was conducted at the application level, with a qualitative-quantitative approach and contributing to a technological development project. It was characterized by four stages: selection of the AI ​​tool, selection of the knowledge base, development of the virtual assistant, validation process and implementation in the clinic. Results: A total of 246 patients with genetic diseases and congenital defects were evaluated. The most predominant genetic category was monogenic genetic syndromes with 223 patients who attended the consultation (90.7%). A success rate of 84.1% was obtained and a success/no success ratio of 4.34. The highest percentage of successes was achieved in monogenic or Mendelian syndromes. There were no significant differences between successes and failures in both chromosomal aberrations and congenital defects of environmental etiology. Conclusions: Through this research, an AI virtual assistant has been validated for the clinical diagnosis of genetic diseases with a high percentage of effectiveness of 84%, which confirms its usefulness to support the clinical diagnosis of cases with genetic diseases