IDENTIFYING APPROPRIATE GERMPLASM FOR PARTICIPATORY BREEDING: AN EXAMPLE FROM THE CENTRAL VALLEYS OF OAXACA, MEXICO

Identifying the appropriate germplasm to be improved is a key component of any participatory breeding effort because of its implications for impacts on social welfare and genetic diversity. This paper describes a method developed to select a subset of 17 populations for a participatory breeding project from a set of 152 maize landraces. The larger set of landraces was collected in order to characterize, for conservation purposes, the maize diversity present in the Central Valleys of Oaxaca, Mexico. The method combines data representing the perspectives of both men and women members of farm households and those of genetic resources specialists, including professional plant breeders, gene bank managers, and social scientists. The different perspectives complement each other. The results show that when the choice of germplasm is based only on the perspective of genetic resources specialists, traits and materials that are important to farm households may be ignored. Such selections may be less valuable to farmers, limiting the impact of the participatory breeding effort on their livelihoods. However, the findings also indicate that relying solely on the perspectives of farm households may lead to lower diversity. Choosing populations based solely on either perspective involves a social cost-either in terms of diversity or in terms of farmer welfare. Although our approach has limitations, many of which are common to participatory research, it represents a systematic method for meeting one of the important challenges of participatory plant breeding.Mexico, Oaxaca, Maize, Zea mays, Land race, Germplasm conservation, Plant breeding, Selecting, Innovation adoption, Social welfare, Welfare economics, On farm research, Participatory research, Farm Management,

Costing the ex situ conservation of genetic resources: maize and wheat at CIMMYT

Worldwide, the number of genebanks and the amount of seed stored in them has increased substantially over the past few decades. Most attention is focused on the likely benefits from conservation, but conserving germplasm involves costs whose nature and magnitude are largely unknown. In this paper we compile and use a set of cost data for wheat and maize stored in the CIMMYT genebank to address a number of questions. What is the cost of storing an accession of either crop for one more year, or, equivalently what is the benefit in terms of cost savings from eliminating duplicate accessions from the genebank? Relatedly, what is the cost from introducing a new accession into the genebank, given the decision to store it is revisited after one year? Does it make economic sense for CIMMYT to discard accessions that may be available elsewhere? As an extension of this line of inquiry it is possible to value the benefits from either consolidating genebanks or at least networking existing banks to reduce or eliminate duplicate holdings not needed for backup safety purposes. We present estimates of the size and scale economies evident in the CIMMYT operation as a basis for assessing the economics of consolidation. Genebanks represent a commitment to conserve seeds for the very long-run. In this study we report on these long-run costs for the CIMMYT genebank costs that are sensitive to the interest rate used and the protocols for periodically replenishing accessions that are shared with others or regenerating accessions whose viability gradually diminishes with age.Germplasm conservation., Gene banks, Plant., Maize Breeding., Wheat Breeding., Rate of return.,

Genetic Characterization of a Core Set of a Tropical Maize Race Tuxpeño for Further Use in Maize Improvement

The tropical maize race Tuxpeño is a well-known race of Mexican dent germplasm which has greatly contributed to the development of tropical and subtropical maize gene pools. In order to investigate how it could be exploited in future maize improvement, a panel of maize germplasm accessions was assembled and characterized using genome-wide Single Nucleotide Polymorphism (SNP) markers. This panel included 321 core accessions of Tuxpeño race from the International Maize and Wheat Improvement Center (CIMMYT) germplasm bank collection, 94 CIMMYT maize lines (CMLs) and 54 U.S. Germplasm Enhancement of Maize (GEM) lines. The panel also included other diverse sources of reference germplasm: 14 U.S. maize landrace accessions, 4 temperate inbred lines from the U.S. and China, and 11 CIMMYT populations (a total of 498 entries with 795 plants). Clustering analyses (CA) based on Modified Rogers Distance (MRD) clearly partitioned all 498 entries into their corresponding groups. No sub clusters were observed within the Tuxpeño core set. Various breeding strategies for using the Tuxpeño core set, based on grouping of the studied germplasm and genetic distance among them, were discussed. In order to facilitate sampling diversity within the Tuxpeño core, a minicore subset of 64 Tuxpeño accessions (20% of its usual size) representing the diversity of the core set was developed, using an approach combining phenotypic and molecular data. Untapped diversity represents further use of the Tuxpeño landrace for maize improvement through the core and/or minicore subset available to the maize community

Comparative SNP and Haplotype Analysis Reveals a Higher Genetic Diversity and Rapider LD Decay in Tropical than Temperate Germplasm in Maize

Understanding of genetic diversity and linkage disequilibrium (LD) decay in diverse maize germplasm is fundamentally important for maize improvement. A total of 287 tropical and 160 temperate inbred lines were genotyped with 1943 single nucleotide polymorphism (SNP) markers of high quality and compared for genetic diversity and LD decay using the SNPs and their haplotypes developed from genic and intergenic regions. Intronic SNPs revealed a substantial higher variation than exonic SNPs. The big window size haplotypes (3-SNP slide-window covering 2160 kb on average) revealed much higher genetic diversity than the 10 kb-window and gene-window haplotypes. The polymorphic information content values revealed by the haplotypes (0.436–0.566) were generally much higher than individual SNPs (0.247–0.259). Cluster analysis classified the 447 maize lines into two major groups, corresponding to temperate and tropical types. The level of genetic diversity and subpopulation structure were associated with the germplasm origin and post-domestication selection. Compared to temperate lines, the tropical lines had a much higher level of genetic diversity with no significant subpopulation structure identified. Significant variation in LD decay distance (2–100 kb) was found across the genome, chromosomal regions and germplasm groups. The average of LD decay distance (10–100 kb) in the temperate germplasm was two to ten times larger than that in the tropical germplasm (5–10 kb). In conclusion, tropical maize not only host high genetic diversity that can be exploited for future plant breeding, but also show rapid LD decay that provides more opportunity for selection

Wellhausen-Anderson Plant Genetic Resources Center Operations Manual 2004

Crop Production/Industries,

Plant genetic resources: What can they contribute toward increased crop productivity?

To feed a world population growing by up to 160 people per minute, with >90% of them in developing countries, will require an astonishing increase in food production. Forecasts call for wheat to become the most important cereal in the world, with maize close behind; together, these crops will account for ≈80% of developing countries’ cereal import requirements. Access to a range of genetic diversity is critical to the success of breeding programs. The global effort to assemble, document, and utilize these resources is enormous, and the genetic diversity in the collections is critical to the world’s fight against hunger. The introgression of genes that reduced plant height and increased disease and viral resistance in wheat provided the foundation for the “Green Revolution” and demonstrated the tremendous impact that genetic resources can have on production. Wheat hybrids and synthetics may provide the yield increases needed in the future. A wild relative of maize, Tripsacum, represents an untapped genetic resource for abiotic and biotic stress resistance and for apomixis, a trait that could provide developing world farmers access to hybrid technology. Ownership of genetic resources and genes must be resolved to ensure global access to these critical resources. The application of molecular and genetic engineering technologies enhances the use of genetic resources. The effective and complementary use of all of our technological tools and resources will be required for meeting the challenge posed by the world’s expanding demand for food

IDENTIFYING APPROPRIATE GERMPLASM FOR PARTICIPATORY BREEDING: AN EXAMPLE FROM THE CENTRAL VALLEYS OF OAXACA, MEXICO

Identifying the appropriate germplasm to be improved is a key component of any participatory breeding effort because of its implications for impacts on social welfare and genetic diversity. This paper describes a method developed to select a subset of 17 populations for a participatory breeding project from a set of 152 maize landraces. The larger set of landraces was collected in order to characterize, for conservation purposes, the maize diversity present in the Central Valleys of Oaxaca, Mexico. The method combines data representing the perspectives of both men and women members of farm households and those of genetic resources specialists, including professional plant breeders, gene bank managers, and social scientists. The different perspectives complement each other. The results show that when the choice of germplasm is based only on the perspective of genetic resources specialists, traits and materials that are important to farm households may be ignored. Such selections may be less valuable to farmers, limiting the impact of the participatory breeding effort on their livelihoods. However, the findings also indicate that relying solely on the perspectives of farm households may lead to lower diversity. Choosing populations based solely on either perspective involves a social cost-either in terms of diversity or in terms of farmer welfare. Although our approach has limitations, many of which are common to participatory research, it represents a systematic method for meeting one of the important challenges of participatory plant breeding

Costing the Ex Situ Conservation of Genetic Resources: Maize and Wheat at CIMMYT

Worldwide, the number of genebanks and the amount of seed stored in them has increased substantially over the past few decades. Most attention is focused on the likely benefits from conservation, but conserving germplasm involves costs whose nature and magnitude are largely unknown. Because more resources spent on conserving germplasm often means less spent on characterizing the collection or using the saved seeds in crop-improvement research, knowledge of the costs of germplasm conservation has important, possibly long run, R&D management, policy, and food-security consequences. Moreover, these costs place a lower bound on the benefits deemed likely to justify the expense of saving this seed. In this paper we compile and use a set of cost data for wheat and maize stored in the CIMMYT genebank to address a number of questions. What is the cost of storing an accession of either crop for one more year, or, equivalently what is the benefit in terms of cost savings from eliminating duplicate accessions from the genebank? Relatedly, what is the cost from introducing a new accession into the genebank, given the decision to store it is revisited after one year? Does it make economic sense for CIMMYT to discard accessions that may be available elsewhere? As an extension of this line of inquiry it is possible to value the benefits from either consolidating genebanks or at least networking existing banks to reduce or eliminate duplicate holdings not needed for backup safety purposes. We present estimates of the size and scale economies evident in the CIMMYT operation as a basis for assessing the economics of consolidation. Genebanks represent a commitment to conserve seeds for the very long-run. In this study we report on these long-run costs for the CIMMYT genebank¾costs that are sensitive to the interest rate used and the protocols for periodically replenishing accessions that are shared with others or regenerating accessions whose viability gradually diminishes with age. We estimate that under baseline assumptions the present value of conserving the existing accessions in perpetuity at CIMMYT is $7.95 million¾$4.42 million for storing the 17,000 maize accessions and $3.53 million for the 123,000 wheat samples. Maintaining the current level of effort to disseminate accessions free-of-charge to those who request them would cost an additional$3.07 million in perpetuity. Contrary to popular perception, conserving seeds (like R&D more generally) is much more of a labor or human-, not physical-capital intensive, undertaking. On an annualized basis, physical capital represents 22 percent of the costs of conservation, labor about 60 percent, with operational costs making up the remaining 18 percent. Much of the labor takes the form of a quasi-fixed input¾the human capital embodied in senior scientific and technical genebank staff is a lumpy labor input that is not especially sensitive to changes in the size of the holding