Structural visualization of key steps in human transcription initiation.

Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function of this assembly have remained elusive due to lack of structural information. Here we have used an in vitro reconstituted system to study the stepwise assembly of human TBP, TFIIA, TFIIB, Pol II, TFIIF, TFIIE and TFIIH onto promoter DNA using cryo-electron microscopy. Our structural analyses provide pseudo-atomic models at various stages of transcription initiation that illuminate critical molecular interactions, including how TFIIF engages Pol II and promoter DNA to stabilize both the closed pre-initiation complex and the open-promoter complex, and to regulate start--initiation complexes, combined with the localization of the TFIIH helicases XPD and XPB, support a DNA translocation model of XPB and explain its essential role in promoter opening

Recent progress in histochemistry

The progress in discerning the structure and function of cells and tissues in health and disease has been achieved to a large extent by the continued development of new reagents for histochemistry, the improvement of existing techniques and new imaging techniques. This review will highlight some advancements made in these field

Measurements and quasi-quantum modeling of the steric asymmetry and parity propensities in state-to-state rotationally inelastic scattering of NO (2?1/2) with D2.

Relative integrated cross sections are measured for spin-orbit-conserving, rotationally inelastic scattering of NO

Histochemistry and Cell Biology: 61 years and not tired at all.

This is the author accepted manuscript. The final version is available from Springer via the DOI in this record.Isaac Newton is credited with quipping, “If I have seen further it is by standing on the shoulders of Giants”. This remark, made more than 300 years ago is still relevant for today’s scientists. Certainly, in our field of Histochemistry and Cell Biology, many of the insights we enjoy and techniques we apply in our research are the result of contributions to the literature provided by our scientific forebearers. As Editors of Histochemistry and Cell Biology, we are entrusted with maintaining the high quality and continued success of the journal instituted by its founders M. Chèvremont, Liège; H.W. Deane, New York; P.B. Diezel, F. Duspiva and H. Reznik, Heidelberg; O. Eränkö, Helsinki; P. Gedigk and N. Schümmelfelder, Bonn; W. Gössner, Tübingen; W. Graumann, Göttingen; A. G. E. Pearse, London; W. Sandritter, Frankfurt/Main; T.H. Schiebler, Kiel; G. Siebert, Mainz; and M. Wolman, Tel-Hashomer. The list of the international editors represented a virtual list of “Who’s Who” in histochemistry at that time.Biotechnology & Biological Sciences Research Council (BBSRC

Conclusions and Recommendations

Rosana G. Moreira, Editor-in-Chief; Texas A&M UniversityThis is a paper from International Commission of Agricultural Engineering (CIGR, Commission Internationale du Genie Rural) E-Journal Volume 7 (2005): Conclusions and Recommendations by E. Gasparett